Innovative Instruments inNO-T Advanced Nitric Oxide Measurement System with amiNO Electrochemical Sensors
| Origin | USA |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | inNO-T System with amiNO Sensor Series |
| Pricing | Upon Request |
Overview
The Innovative Instruments inNO-T System is a purpose-engineered, battery-operated electrochemical platform for real-time, quantitative detection of free nitric oxide (NO) in complex biological matrices. It integrates a 24-bit analog-to-digital converter (16,777,216:1 resolution) capable of resolving currents as low as 0.1 pA—enabling sub-nanomolar (sub-1 nM) NO concentration measurements with high signal fidelity. The system operates on the principle of amperometric detection: NO diffuses selectively through a proprietary triple-layer gas-permeable membrane and undergoes oxidation at a catalytic electrode surface, generating a current linearly proportional to NO concentration. This design eliminates dependence on reference or counter electrodes, resulting in a compact, integrated sensor architecture optimized for in vitro, ex vivo, and in vivo applications—including cell monolayers, perfused organs, and intratissue microprobing. Unlike conventional NO sensors limited by membrane fragility, baseline drift, and poor selectivity, the inNO-T platform delivers reproducible, low-drift performance under physiological conditions, meeting core requirements for GLP-compliant preclinical research and mechanistic pharmacology studies.
Key Features
- 24-bit high-resolution data acquisition enabling detection limits <1 nM NO
- Integrated amiNO sensor series—fully shielded from electromagnetic and thermal noise
- Proprietary TRIPLECOAT membrane technology ensuring exceptional selectivity against O₂, CO₂, CO, NO₂⁻, NO₃⁻, and reactive nitrogen species
- Battery-powered portable design (up to 8 hours continuous operation) for intraoperative and field-deployable use
- Onboard magnetic stirrer and calibration kit included for standardized assay setup
- Zero-background correction, real-time scaling, and programmable sampling rates (0.1–10 Hz)
- On-the-fly calculation of Min/Max/Delta/Average/Integral for kinetic analysis
Sample Compatibility & Compliance
The amiNO sensor series is validated for direct measurement in diverse sample formats: adherent mammalian cell cultures (e.g., endothelial monolayers), isolated perfused organs (heart, kidney, liver), acute brain slices, vascular tissue rings, and in vivo rodent models (cerebral cortex, skeletal muscle, bladder). All sensors comply with ISO 13485 design control principles for medical device components and support FDA 21 CFR Part 11–compliant data integrity when used with audit-trail-enabled software configurations. Calibration traceability follows NIST-traceable nitrite standards via acidified iodide conversion (1:1 stoichiometry), satisfying ASTM E2912-13 guidelines for electrochemical sensor validation in biological fluids.
Software & Data Management
The inNO-T Analysis Software (Windows™ 95–NT compatible) provides full instrument control, visualization, and post-acquisition processing. It supports dual-channel synchronous recording, time-stamped metadata logging (temperature, pH, stir speed), and export to CSV or bitmap formats. Data files retain embedded calibration parameters and sensor serial identifiers—critical for longitudinal study reproducibility. Exported text files are directly importable into MATLAB®, GraphPad Prism®, or Microsoft Excel™ without format loss. Audit trails record all user-initiated parameter changes, file saves, and calibration events, fulfilling GLP/GMP documentation requirements for regulated laboratories.
Applications
The inNO-T System supports quantitative NO dynamics in:
- Endothelial NO synthase (eNOS) activity assays under shear stress or agonist stimulation
- Neuronal NO release during LTP/LTD induction in hippocampal slices
- Inflammatory macrophage iNOS kinetics in response to LPS/IFN-γ
- Pharmacological evaluation of NO donors (e.g., DEA/NO, SNAP) and NOS inhibitors (L-NAME, 1400W)
- Real-time monitoring of NO-mediated vasodilation in pressurized arterioles
- Translational biomarker studies linking tissue NO flux to oxidative stress markers (e.g., 3-nitrotyrosine)
FAQ
What is the inNO-T System?
The inNO-T System is a fully integrated, computer-controlled nitric oxide measurement platform comprising a 24-bit potentiostat, amiNO electrochemical sensors, magnetic stirrer, and calibration reagents—all housed in a portable, battery-operated unit.
How does the amiNO sensor achieve selectivity for NO?
Selectivity arises from the TRIPLECOAT membrane’s differential gas permeability and electrode surface catalysis—rejecting >99.9% of interferents including O₂, CO, NO₂⁻, and H₂O₂ while permitting quantitative NO diffusion and oxidation.
What is the typical response time of amiNO sensors?
Response time (t₉₀) ranges from <1 second (amiNO-30, amiNO-7) to ~3 seconds (amiNO-FLAT), depending on membrane thickness and active surface area.
Can amiNO sensors measure nitrite or nitrate directly?
No—nitrite is quantified indirectly via acidified iodide conversion to NO; nitrate requires prior enzymatic reduction to nitrite using the optional Nitrate Reductor module.
What is the expected operational lifetime of an amiNO sensor?
Under standard handling protocols, amiNO-2000 lasts >2 years (with user-replaceable membranes); amiNO-IV and amiNO-FLAT maintain functional stability for ≥12 months in tissue penetration or monolayer assays.
Is the inNO-T System compatible with third-party data acquisition hardware?
Yes—via BNC analog output and optional adapter cables, though full functionality (e.g., auto-calibration, drift compensation) is guaranteed only with the native inNO-T meter.
