Jasco LC-2000Plus Used High-Performance Liquid Chromatograph

Overview

The Jasco LC-2000Plus is a fully integrated, modular high-performance liquid chromatography (HPLC) system originally engineered by Jasco Inc. (Japan Spectroscopic Co., Ltd.) for precision separation, quantification, and identification of thermally labile, non-volatile, and polar compounds. Built upon over three decades of HPLC platform development, the LC-2000Plus employs low-pressure quaternary gradient mixing architecture—enabling precise solvent composition control across four mobile phase channels—and supports isocratic, step-gradient, and linear gradient elution modes. Its compact footprint, robust fluidic design, and thermally stabilized optical path make it suitable for routine QC analysis, pharmaceutical stability testing, natural product profiling, and environmental contaminant screening in regulated and research laboratories. This unit has undergone full functional refurbishment and performance verification at SpectraLab Scientific’s ISO/IEC 17025-aligned service facility, including baseline noise assessment, retention time reproducibility (RSD 0.999 over 3–4 orders of magnitude).

Key Features

• PU-2089Plus quaternary low-pressure gradient pump: Delivers flow rates from 0.001 to 10.000 mL/min with ≤0.1% RSD flow precision and pulseless delivery; pressure range up to 40 MPa (5800 psi)
• AS-2059Plus autosampler: 100-position vial capacity, 0.1–100 µL injection volume range, programmable needle wash, and temperature-controlled sample compartment (4–40 °C)
• CO-2060Plus column oven: Maintains temperature stability of ±0.1 °C across 5–85 °C operating range; accommodates columns up to 250 mm in length with dual-zone air circulation
• FP-2020Plus fluorescence detector: Excitation/emission wavelength range 200–650 nm, spectral bandwidth 10 nm, and signal-to-noise ratio ≥1000:1 (quinine sulfate in 0.1 M H₂SO₄)
• DG-2080-54 membrane-based degasser: Four independent vacuum channels with real-time pressure monitoring and automatic leak detection
• C-Net II v3.x chromatography data system: Supports dual-channel analog/digital acquisition, peak integration per USP , and audit-trail-enabled method storage compliant with FDA 21 CFR Part 11 requirements when deployed on validated Windows OS environments

Sample Compatibility & Compliance

The LC-2000Plus accommodates reversed-phase (C18, C8), normal-phase (silica, cyano), ion-exchange, and size-exclusion columns with internal diameters from 2.1 mm to 4.6 mm and lengths up to 250 mm. It is routinely deployed for analysis of small-molecule pharmaceuticals (e.g., APIs, impurities per ICH Q3B), peptides, nucleotides, vitamins, pesticides (EPA Method 531.1), and polyphenols. All refurbished units are verified against Jasco’s original factory specifications and documented per GLP-compliant work instructions. System suitability testing (SST) templates—including tailing factor, resolution, and theoretical plates—are preloaded in C-Net II. The instrument meets mechanical and electrical safety requirements per IEC 61010-1 and electromagnetic compatibility standards per EN 61326-1.

Software & Data Management

C-Net II software provides method development tools (gradient optimization, dwell volume correction), real-time chromatogram overlay, and customizable report generation with embedded metadata (operator ID, instrument serial number, calibration date). Raw data files (.adc) are stored in vendor-native binary format with embedded timestamps and checksums. When installed on a locked-down Windows 10/11 workstation with domain authentication and encrypted storage, the system supports electronic signatures, user role assignment (admin/operator/auditor), and full audit trail export (CSV or PDF) for regulatory submissions. Data integrity is further ensured through automatic backup scheduling and write-once-read-many (WORM) archive options.

Applications

• Pharmaceutical QC: Assay and related substances testing per USP monographs and EP 2.2.46
• Food safety: Detection of mycotoxins (aflatoxin B1), preservatives (sorbic acid), and artificial colors (Allura Red AC)
• Environmental analysis: Quantification of PAHs in soil extracts and chlorinated phenols in wastewater
• Academic research: Separation of flavonoid glycosides in plant extracts using gradient elution and fluorescence detection
• Biochemical characterization: Monitoring enzymatic reaction kinetics via post-column derivatization and fluorescence readout

FAQ

Is this unit covered under manufacturer warranty?
No. As a refurbished second-hand instrument, it carries a 6-month limited warranty administered by SpectraLab Scientific, covering defects in materials and workmanship under normal laboratory use.
Can the system be validated for GMP compliance?
Yes. Documentation packages—including IQ/OQ protocols, calibration certificates, and system suitability test records—are available upon request. Full 21 CFR Part 11 compliance requires customer-side IT infrastructure validation.
What consumables and spare parts are included?
The unit ships with all original modules, power cables, communication interfaces, and the complete C-Net II software license. No columns, solvents, or vials are included.
Is remote support available after purchase?
Yes. SpectraLab Scientific provides email-based technical assistance and optional paid remote desktop sessions for method troubleshooting and software configuration.
How is performance verification documented?
Each unit receives a Certificate of Refurbishment and Performance Verification, listing measured parameters (flow accuracy, gradient precision, detector sensitivity, column oven stability) against Jasco LC-2000Plus specification limits, signed by a qualified service engineer.