Jiapeng DHL-C Digital Constant Flow Peristaltic Pump

Overview

The Jiapeng DHL-C Digital Constant Flow Peristaltic Pump is an engineered solution for precise, repeatable liquid handling in analytical and preparative chromatography workflows. Based on the positive displacement principle of peristaltic pumping—where rollers compress flexible tubing to generate unidirectional fluid motion—the DHL-C delivers stable, pulse-minimized flow without contact between pump mechanism and fluid. This design ensures contamination-free operation, making it ideal for sensitive applications including nucleic acid and protein purification, gradient elution in low-pressure liquid chromatography (LPLC), and automated fraction collection systems. Its dual-channel architecture supports parallel or independent flow control, enabling synchronized delivery to multiple columns or simultaneous sampling from two sources. Designed for integration with UV/Vis detectors (e.g., nucleic acid/protein analyzers) and programmable fraction collectors, the DHL-C serves as a core fluidic actuator in benchtop chromatographic separation platforms compliant with GLP-aligned laboratory practices.

Key Features

• 3.5-inch high-resolution capacitive touchscreen LCD with intuitive Chinese-language interface—displays real-time flow rate, rotational speed (rpm), direction, operational status, and elapsed time.
• Dual independent peristaltic channels, each equipped with a precision-machined 6-roller pump head for smooth, low-pulsation flow across the full range of 0.1–1099 mL/h per channel.
• 256-microstep stepper motor drive ensures exceptional speed stability, minimal torque ripple, and reliable bidirectional operation (forward/reverse) at speeds from 0.1 to 75 rpm.
• Integrated flow calibration routine: users measure actual delivered volume over a defined interval, input the result, and the system automatically adjusts internal flow coefficients—enhancing long-term volumetric accuracy without hardware modification.
• Dedicated “Full-Speed” mode enables rapid tubing priming, air bubble removal, and system purge—activated via single-key command.
• TTL-compatible external control interface allows direct synchronization with fraction collectors: pump start/stop signals trigger collector fraction switching, supporting time- or volume-based collection protocols.
• Enclosure constructed from flame-retardant, medical-grade ABS polymer via precision injection molding—robust, chemical-resistant, and ergonomically compact (125 × 150 × 180 mm).

Sample Compatibility & Compliance

The DHL-C accommodates standard peristaltic tubing sizes Ø1.2 × 3 mm and Ø3 × 5 mm, compatible with pharmaceutical-grade silicone, Viton®, and Pharmed® BPT tubing—validated for aqueous buffers, organic solvents (e.g., acetonitrile, methanol ≤30%), and mild acidic/basic solutions (pH 2–12). Maximum pressure rating of ≥3 kg/cm² (≈294 kPa) supports operation under backpressure typical of packed columns (e.g., Sephadex®, DEAE-cellulose) and inline filters. While not certified to IEC 61010-1 or ISO 13485 out-of-box, the unit meets essential safety requirements for Class I laboratory equipment operating at 220 V AC. Its closed-loop flow control architecture aligns with principles referenced in USP , ASTM D4785, and ISO 11648-2 for quantitative liquid delivery in analytical method validation contexts.

Software & Data Management

The DHL-C operates as a standalone instrument with embedded firmware—no PC software required for basic operation. All parameters (flow rate, duration, volume, distribution count, pause intervals) are configured directly via touchscreen. For traceability in regulated environments, the device logs operational history—including start/stop timestamps, total dispensed volume per channel, and calibration events—in non-volatile memory. Though lacking native network connectivity or FDA 21 CFR Part 11-compliant electronic signatures, its deterministic behavior, manual calibration audit trail, and reproducible output support documentation within laboratory notebooks or LIMS-integrated workflows adhering to ALCOA+ data integrity principles.

Applications

• Preparative gel filtration and ion-exchange chromatography for protein/nucleic acid isolation.
• Automated buffer exchange and column equilibration in affinity purification setups.
• Controlled reagent addition in enzymatic assays and kinetic studies.
• Continuous sampling for online UV absorbance monitoring in tandem with nucleic acid/protein detectors.
• Low-flow perfusion in cell culture media exchange or microfluidic interface priming.
• Calibration standard delivery in volumetric method verification per ISO/IEC 17025 Clause 7.7.

FAQ

What tubing types are recommended for optimal performance and chemical resistance?
Silicone tubing (for aqueous buffers) and Pharmed® BPT (for mixed aqueous/organic mobile phases up to 30% organic content) are validated choices. Avoid PVC or latex in precision applications due to swelling and creep.
Can the DHL-C maintain consistent flow under variable backpressure?
Yes—its stepper motor feedback loop and 256-step microdriving provide stable rotational velocity across the specified pressure range (≤294 kPa); however, flow deviation may exceed ±2% above 3 kg/cm².
Is remote computer control supported via USB or RS232?
No—control is limited to front-panel touchscreen and TTL-level digital I/O for fraction collector triggering. No serial or USB host interface is provided.
How frequently should flow calibration be performed?
Recommended before first use, after tubing replacement, and at least once per week during continuous operation—or whenever observed volumetric drift exceeds ±1.5% of setpoint.
Does the pump support gradient programming (e.g., linear flow ramping)?
No—flow rate is fixed per run. Gradient formation requires external coordination with a second pump or programmable valve manifold.