Jingfei FLB1007 Quadruple Cuvette Holder for Spectroscopic Measurements

Overview

The Jingfei FLB1007 Quadruple Cuvette Holder is an engineered optical mounting platform designed for multi-directional spectroscopic interrogation of liquid or semi-transparent solid samples in cuvettes. It implements a fixed-path, four-port orthogonal geometry—each port aligned along the X, Y, +Z, and −Z axes—enabling simultaneous or sequential excitation and detection configurations without mechanical repositioning. This architecture supports fundamental photometric measurement modalities including absorbance (transmission), fluorescence excitation/emission, Rayleigh and Mie scattering, and hybrid optical assays where multiple phenomena occur concurrently. The device operates on passive optical coupling principles: no internal electronics or active alignment mechanisms are integrated; instead, it relies on precision-machined kinematic mounts and factory-aligned collimating lenses to ensure repeatable beam positioning and minimal angular deviation (< ±0.15° typical). Its design conforms to standard 12.5 × 12.5 mm square cuvette footprints and maintains strict 10 mm pathlength consistency across all illumination/detection paths—critical for quantitative Beer–Lambert law calculations and comparative spectral normalization.

Key Features

• Four independently accessible optical ports with integrated collimating lenses (f = 8 mm, NA 0.22), each fitted with SMA905 fiber-optic connectors for low-loss coupling to spectrometers, LEDs, lasers, or broadband sources
• Rigid black anodized aluminum base (6061-T6 alloy) providing thermal stability (CTE: 23.6 µm/m·K), EMI shielding, and resistance to laboratory solvents and mild acids
• Mechanically constrained cuvette cavity with spring-loaded retention and ±0.05 mm positional repeatability—ensuring consistent beam–sample interaction across repeated insertions
• No moving parts or motorized components; optimized for long-term calibration stability and minimal maintenance in GLP-compliant environments
• Compatible with standard 10 mm pathlength quartz, glass, or UV-grade fused silica cuvettes (external dimensions: 12.5 × 12.5 × 45 mm)

Sample Compatibility & Compliance

The FLB1007 accommodates aqueous, organic, and viscous solutions—including suspensions up to 5% w/v particulate loading—without flow requirements or pressure sealing. Its open-top design permits rapid sample exchange and visual verification of meniscus height and clarity. While not rated for pressurized or high-temperature operation (>60 °C), it meets mechanical robustness benchmarks per ISO 17025:2017 Annex A.3 for ancillary optical hardware used in accredited testing laboratories. When deployed with NIST-traceable light sources and calibrated spectrometers, measurements performed using this holder satisfy data integrity expectations under FDA 21 CFR Part 11 when paired with compliant software platforms supporting electronic signatures and audit trails.

Software & Data Management

As a passive optical interface, the FLB1007 does not include embedded firmware or native software drivers. It functions transparently within established spectroscopy ecosystems—including Ocean Insight OceanView, Avantes ASE, or custom LabVIEW/Python-based acquisition frameworks. Experimental configuration (e.g., excitation-detection channel mapping, background subtraction sequences, kinetic time-point triggering) is fully managed at the spectrometer or source controller level. Raw spectral datasets retain full metadata linkage to the physical setup (e.g., “FLB1007_Port_Z+_SMA_Coupled_to_365_nm_LED”), enabling traceable method documentation per ICH Q5C and USP guidelines.

Applications

• Multi-angle fluorescence quantum yield determination using reference standards (e.g., quinine sulfate in 0.1 M H₂SO₄)
• Dual-beam ratiometric absorbance monitoring for real-time reaction kinetics (e.g., enzyme-catalyzed hydrolysis)
• Anisotropic scattering profiling of colloidal nanoparticles (e.g., gold nanospheres, TiO₂ dispersions)
• Validation of optical pathlength uniformity across spectrometer detector arrays
• Teaching laboratories: hands-on demonstration of Jablonski diagrams, inner-filter effects, and polarization-dependent absorption

FAQ

Can the FLB1007 be used with UV-VIS-NIR spectrometers covering 200–1100 nm?
Yes—provided collimating lenses and coupled fibers are specified for the target wavelength range (e.g., UV-grade fused silica lenses and solarization-resistant fibers for <250 nm operation).
Is cuvette height adjustment possible?
No—the holder fixes vertical position to maintain 10 mm pathlength; users must select cuvettes with appropriate fill height (typically 3.5–4.0 cm minimum).
Does the base include tapped holes for optical table mounting?
Yes—four M4 threaded holes (pitch 0.7 mm) are symmetrically located at the base corners, compatible with standard kinematic mounts and translation stages.
Are replacement collimating lenses available as spare parts?
Yes—Jingfei offers lens assemblies (part number FLB-LNS-08-UVVIS) with AR coatings optimized for 350–800 nm transmission.
Can this holder support polarization-resolved measurements?
Yes—when combined with external linear polarizers mounted upstream of each SMA port, it enables controlled polarization state selection for dichroism or birefringence studies.