Junyi JY-SP3 High-Throughput Horizontal Electrophoresis System
| Brand | Junyi |
|---|---|
| Origin | Beijing, China |
| Model | JY-SP3 |
| Instrument Type | Nucleic Acid Electrophoresis Apparatus |
| Gel Tray Dimensions (W×L) | 85 × 200 mm |
| Sample Capacity | 216 wells (compatible with 96-well PCR plate layout, dual-gel configuration) |
| Buffer Volume | ~50 mL |
| External Dimensions (L×W×H) | 300 × 150 × 61 mm |
| Net Weight | 0.5 kg |
| Gel Thickness | 1.5 mm |
| Compatible Comb Configurations | 10- and 18-tooth combs |
Overview
The Junyi JY-SP3 High-Throughput Horizontal Electrophoresis System is a compact, safety-engineered platform designed for rapid nucleic acid separation in high-volume molecular biology workflows. Operating on the principle of horizontal agarose or polyacrylamide gel electrophoresis, the system enables migration of DNA fragments under an applied electric field—though it is supplied as a standalone electrophoresis chamber requiring external power supply (not integrated). Its optimized buffer chamber geometry and low-profile gel tray ensure uniform field distribution and minimal thermal gradient across the gel surface, supporting reproducible band resolution and reduced run time. Engineered specifically for compatibility with standard 96-well PCR plate formats, the JY-SP3 supports parallel processing of two gels—each accommodating up to 108 samples—making it ideal for applications demanding rapid turnaround without compromising lane integrity or loading precision.
Key Features
- High-throughput sample capacity: 216 total wells (two 108-well gels), configured to mirror 96-well microplate spacing for seamless integration with automated liquid handling and manual multichannel pipetting.
- Optimized gel dimensions: 85 mm (width) × 200 mm (length) tray accommodates standard 1.5 mm thick gels, ensuring mechanical stability during casting and electrophoresis while maximizing usable separation length.
- Integrated safety interlock: Lid-mounted microswitch automatically interrupts electrical continuity upon opening—fully compliant with IEC 61010-1 Class II safety requirements for laboratory electrical equipment.
- Contrast-enhancing black baseplate: Non-reflective matte surface improves visual detection of wells during loading and real-time monitoring of dye front migration under ambient or UV transillumination.
- Ergonomic access design: Front-mounted release button enables one-handed lid operation, minimizing accidental gel disturbance and reducing operator fatigue during high-frequency use.
- Low thermal mass architecture: Compact footprint (300 × 150 × 61 mm) and minimal buffer volume (~50 mL) contribute to rapid equilibration and stable voltage application during short-run protocols.
Sample Compatibility & Compliance
The JY-SP3 is validated for use with standard agarose (0.7–3.0%) and native or denaturing polyacrylamide gels (up to 20%). It supports common nucleic acid staining agents including ethidium bromide, SYBR Safe, GelRed, and GelGreen. The system conforms to general laboratory safety standards for non-powered electrophoresis chambers per ISO 15190:2020 (Laboratory medicine — Requirements for safety) and aligns with Good Laboratory Practice (GLP) documentation expectations for equipment qualification. While not a regulated medical device, its design facilitates traceable setup logging when paired with external power supplies meeting IEC 61010-2-040 (Electrophoresis equipment particular requirements).
Software & Data Management
As a passive electrophoresis chamber, the JY-SP3 does not include embedded firmware or proprietary software. However, its standardized physical dimensions and well geometry ensure full interoperability with third-party gel documentation systems—including digital gel imagers (e.g., Azure Biosystems cSeries, Bio-Rad GelDoc EZ), image analysis platforms (ImageLab, Quantity One, Fiji/ImageJ), and LIMS-integrated workflows. When used in regulated environments (e.g., clinical PCR labs), users may implement audit-trail-enabled imaging software compliant with FDA 21 CFR Part 11 for electronic record retention, provided the external imaging hardware and software meet applicable validation criteria.
Applications
- Routine PCR product verification and amplicon sizing in diagnostic and research laboratories.
- High-volume primer validation and screening during assay development.
- Genotyping workflows involving multiplex PCR and allele-specific amplification.
- Quality control of plasmid preps, restriction digests, and RT-PCR products.
- Teaching laboratory environments where safety, speed, and visual clarity are pedagogically prioritized.
- Field-deployable molecular testing setups where portability and low power dependency are critical.
FAQ
Does the JY-SP3 include a power supply?
No. The JY-SP3 is a gel electrophoresis chamber only and requires connection to an external DC power supply capable of delivering 0–300 V and 0–500 mA.
Can I cast gels directly in the JY-SP3 tray?
Yes. The tray features integrated comb slots and a sealed buffer chamber suitable for in-situ gel polymerization using standard agarose or acrylamide solutions.
Is the JY-SP3 compatible with UV transilluminators?
Yes. Its low-profile design and transparent lid (optional accessory) allow direct placement on standard UV or blue-light transilluminators for post-electrophoresis visualization.
What comb configurations are supported?
The system accepts interchangeable 10-tooth and 18-tooth combs (1.5 mm thickness), enabling flexible well density selection based on sample volume and resolution requirements.
How is electrical safety ensured during operation?
A certified mechanical interlock interrupts circuit continuity at the electrode terminals whenever the lid is opened—eliminating risk of accidental contact with live electrodes.
