Junyi JY-SPAT Horizontal Electrophoresis Chamber
| Brand | Junyi |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | JY-SPAT |
| Instrument Type | Transfer Electrophoresis Apparatus |
| Primary Component | Electrophoresis Chamber |
| Gel Dimensions (W×L) | 48×75 mm |
| Sample Capacity | 18 wells |
| Buffer Volume | ~150 mL |
| External Dimensions (L×W×H) | 250×90×50 mm |
| Net Weight | 0.4 kg |
| Gel Thickness Options | 1.0 mm and 1.5 mm |
| Comb Configurations | 3-, 5-, and 9-well combs for both thicknesses |
| Material | Imported high-transparency polycarbonate (PC), one-piece injection molded |
| Safety Features | Lid-integrated automatic circuit cutoff, ergonomic handle-equipped gel tray, color-contrasted loading background, removable electrode assembly, dedicated gel casting comb set |
Overview
The Junyi JY-SPAT Horizontal Electrophoresis Chamber is a compact, CE-compliant benchtop system engineered for routine nucleic acid separation and molecular weight estimation in academic, clinical, and quality control laboratories. It operates on the principle of horizontal agarose or polyacrylamide gel electrophoresis, where DNA fragments migrate under an applied electric field through a porous matrix, resolving by size-dependent mobility. Unlike vertical systems, the JY-SPAT’s horizontal configuration enables rapid gel preparation, straightforward sample loading, and direct post-run UV visualization without gel transfer—making it especially suited for rapid genotyping, PCR product verification, restriction digest analysis, and educational applications requiring reproducible, low-complexity workflows.
Key Features
- One-piece injection-molded polycarbonate chamber body ensures optical clarity, chemical resistance to common electrophoresis buffers (TAE, TBE, SB), and mechanical durability across repeated thermal cycles.
- Integrated safety interlock: The lid contains embedded microswitches that automatically de-energize the electrode circuit upon opening—complying with IEC 61010-1 requirements for laboratory electrical equipment.
- Ergonomic gel tray with integrated side handles facilitates secure handling during gel pouring, loading, and post-electrophoresis extraction—minimizing risk of gel fracture or buffer spillage.
- High-contrast background surface on the gel tray improves pipetting accuracy during sample loading, particularly under ambient lab lighting conditions.
- Modular electrode assembly allows tool-free removal and replacement of platinum-coated stainless-steel electrodes, supporting long-term maintenance and consistent current distribution.
- Includes a dedicated reusable gel casting comb set compatible with both 1.0 mm and 1.5 mm gel thicknesses, enabling flexible resolution tuning for fragments ranging from 100 bp to >20 kbp.
Sample Compatibility & Compliance
The JY-SPAT accommodates standard agarose gels (0.7–2.0% w/v) and native or denaturing polyacrylamide gels (3–12% T). Its 48 × 75 mm gel format supports up to 18 samples per run when using 9-well combs (1.5 mm thickness), with linear resolution validated per ISO 13485-aligned internal QC protocols. Buffer volume (~150 mL) maintains stable pH and temperature during typical 30–60 min runs at constant voltage (used with external power supply). The device meets general safety requirements outlined in EN 61010-1:2010 and is compatible with GLP-compliant documentation practices when paired with traceable power supplies and calibrated UV transilluminators.
Software & Data Management
As a standalone electrophoresis chamber, the JY-SPAT does not incorporate onboard electronics or firmware. It is designed for integration into existing laboratory workflows utilizing external DC power supplies (e.g., 0–300 V, 0–500 mA output) and digital imaging systems. Gel documentation may be performed using third-party gel documentation stations compliant with FDA 21 CFR Part 11 (when equipped with audit trail and electronic signature modules). Raw image files (TIFF, PNG) generated from such systems can be imported into open-source tools (ImageJ/Fiji) or commercial analysis suites (Bio-Rad Quantity One, TotalLab Quant) for band intensity quantification, molecular weight calibration, and comparative densitometry.
Applications
- Routine verification of PCR amplification products and cloning constructs.
- Assessment of restriction enzyme digestion efficiency and fragment mapping.
- Quality control testing of plasmid preps and genomic DNA integrity (e.g., presence of shearing or degradation).
- Teaching laboratory use for foundational molecular biology techniques—including gel pouring, loading, electrophoresis, and ethidium bromide or SYBR Safe staining.
- Preliminary sizing of dsDNA ladders (e.g., 100 bp DNA Ladder, Lambda HindIII Digest) with ±5% accuracy under optimized buffer and voltage conditions.
FAQ
Does the JY-SPAT include a built-in power supply?
No. It is a passive electrophoresis chamber requiring connection to an external DC power supply capable of delivering stable voltage and current outputs.
Is the gel tray autoclavable?
The polycarbonate tray is not autoclavable; repeated exposure to 121°C steam will cause deformation and loss of optical clarity. Clean with 70% ethanol or mild detergent solutions only.
Can I use this chamber for SDS-PAGE?
No. The JY-SPAT is optimized for nucleic acid separation in horizontal configuration and lacks the cooling capacity, gel thickness compatibility, or electrode geometry required for protein electrophoresis.
What buffer volumes are recommended for optimal performance?
A minimum of 120 mL and maximum of 160 mL of running buffer (TAE or TBE) is recommended to ensure full submersion of the gel and uniform field distribution across the active area.
Are replacement combs available separately?
Yes. Junyi offers OEM-compatible 3-, 5-, and 9-well combs for both 1.0 mm and 1.5 mm gel thicknesses as consumable accessories under part numbers JY-CB-3T, JY-CB-5T, and JY-CB-9T.
