KEZHE PuriMaster-7000 Multidimensional Preparative Liquid Chromatography System
| Brand | KEZHE SHANGHAI |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Direct Manufacturer |
| Product Category | Domestic |
| Model | PuriMaster-7000 |
| Application Level | Laboratory Grade |
| Instrument Type | High-Pressure Preparative Liquid Chromatograph |
| Flow Rate Range | 0–100 mL/min (customizable up to 200 mL/min) |
| Flow Accuracy | RSD ≤ 0.5% |
| Flow Precision | RSD ≤ 1.0% |
| Maximum Pressure | 4000 psi (27.6 MPa) |
| Wavelength Range | 190–850 nm (quad-wavelength simultaneous detection) |
| Wavelength Accuracy | ±0.2 nm |
| Wavelength Repeatability | ±0.4 nm |
| Baseline Noise | ≤3 × 10⁻⁵ AU |
| Data Acquisition Rate | 5 Hz |
| Autosampler Capacity | 42 or 144 positions |
| Fraction Collector Capacity | 160 × 15 × 150 mm test tubes |
Overview
The KEZHE PuriMaster-7000 Multidimensional Preparative Liquid Chromatography System is an engineered platform for high-resolution, scalable purification of complex organic and biomolecular mixtures in research and development laboratories. Built upon a high-pressure quaternary gradient pumping architecture, the system implements orthogonal separation dimensions—either sequentially (1D) or orthogonally coupled (2D)—to significantly expand peak capacity beyond conventional preparative HPLC. Its core design follows the principles of comprehensive two-dimensional liquid chromatography (LC×LC), where effluent from a first-dimension column is actively modulated via a high-speed, low-dead-volume switching valve and transferred to a second-dimension column with complementary selectivity (e.g., reversed-phase × hydrophilic interaction or size exclusion). This architecture enables resolution enhancement by orders of magnitude for natural product isolations, synthetic impurity profiling, peptide mapping, and pharmaceutical intermediate purification—particularly where co-elution limits downstream characterization or biological testing.
Key Features
- High-pressure quaternary gradient pump system delivering stable flow from 0–100 mL/min (extendable to 200 mL/min upon request), with flow accuracy ≤0.5% RSD and precision ≤1.0% RSD across the full range.
- Quad-wavelength UV-Vis detector operating from 190–850 nm, supporting simultaneous multi-wavelength monitoring at user-defined wavelengths; wavelength accuracy ±0.2 nm, repeatability ±0.4 nm, baseline noise ≤3 × 10⁻⁵ AU at 254 nm (1 s response time).
- Modular 2D switching valve system with <10 µL internal volume and <100 ms actuation time, enabling precise heart-cutting or comprehensive LC×LC operation without band broadening.
- Sealed-loop autosampler with 42- or 144-position capacity, integrated needle wash station using dual-solvent rinse protocol to eliminate carryover (<0.005% typical), compatible with standard 12 × 32 mm vials.
- Intelligent fraction collector accommodating up to 160 positions of 15 × 150 mm test tubes; programmable by time, volume, or UV threshold triggers with real-time retention time alignment.
- Modular liquid chromatography workstation housing all components—including solvent reservoirs, degasser, column oven (ambient to 60 °C), and system control electronics—in a compact, service-accessible chassis.
Sample Compatibility & Compliance
The PuriMaster-7000 supports a broad range of sample matrices, including small-molecule APIs, natural extracts, oligonucleotides, peptides, and polar metabolites. Column compatibility extends to standard stainless-steel or PEEK-lined preparative columns (up to 25 mm ID), with included C18 column (Φ10 × 250 mm, 10 µm silica) optimized for mid-scale purification (1–50 mg injections). All fluidic pathways are chemically resistant to common organic solvents (acetonitrile, methanol, THF, DCM) and aqueous buffers (pH 2–9). The system meets mechanical and electrical safety requirements per IEC 61010-1 and electromagnetic compatibility standards EN 61326-1. While not pre-certified for GMP production, its architecture supports audit-ready operation under GLP environments: method parameters are stored with timestamps, fraction collection logs include UV trace metadata, and software enforces user-level access controls consistent with FDA 21 CFR Part 11 readiness when deployed with compliant IT infrastructure.
Software & Data Management
The PuriMaster Control Suite is a Windows-based application offering intuitive workflow-driven operation—from method setup and gradient programming to real-time chromatogram visualization and fraction triggering logic. It supports method serialization, version-controlled storage, and export of raw data in open formats (e.g., .csv, .cdf). All acquisition events—including pump pressure, UV absorbance, valve position status, and fraction trigger timestamps—are logged with millisecond resolution. Audit trail functionality records operator logins, parameter changes, and run completions. Data integrity safeguards include electronic signatures for critical actions (e.g., method validation, batch release), configurable password policies, and automatic backup to network drives. Integration with LIMS is supported via OPC UA and RESTful API endpoints for automated sample registration and result ingestion.
Applications
- Purification of synthetic reaction mixtures containing regioisomers or diastereomers where one-dimensional separation yields insufficient resolution.
- Isolation of minor alkaloids or flavonoids from plant extracts requiring orthogonal selectivity (e.g., C18 × NH₂ or phenyl-hexyl coupling).
- Preparative-scale separation of monoclonal antibody fragments (Fab, Fc) using SEC × RP workflows.
- Method development for clinical trial material synthesis, where reproducibility across batches must meet ICH Q5A/Q5B expectations.
- Supporting structural elucidation pipelines: collecting clean fractions for subsequent NMR, HRMS, or bioassay without offline re-injection or manual pooling.
FAQ
What is the maximum column inner diameter supported by the PuriMaster-7000?
The system is validated for columns up to 25 mm ID; flow dynamics and pressure management remain stable within specified limits at full rated flow.
Can the quad-wavelength detector be used for simultaneous quantitation of multiple analytes?
Yes—four independent photodiode arrays enable concurrent measurement at user-selected wavelengths, allowing ratio-based quantitation (e.g., peak purity assessment) or dual-reference monitoring.
Is the system compatible with volatile mobile phases for LC-MS coupling downstream?
All wetted parts are compatible with MS-friendly solvents (e.g., formic acid/water, ammonium acetate/acetonitrile); optional ESI interface kits are available for direct coupling to mass spectrometers.
How is method transfer ensured between analytical and preparative scales?
The software includes retention time normalization tools and linear velocity scaling calculators to maintain k’ and α values during scale-up, aligned with USP guidance on chromatographic system suitability.
Does the system support gradient delay compensation for 2D operation?
Yes—the valve timing module automatically adjusts second-dimension injection windows based on first-dimension elution profiles, minimizing modulation-induced band distortion.
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