LuXiangYi DDL-5M Low-Speed Refrigerated Centrifuge

Overview

The LuXiangYi DDL-5M Low-Speed Refrigerated Centrifuge is an engineered floor-standing instrument designed for high-capacity, temperature-controlled sedimentation of biological suspensions in routine laboratory workflows. Operating on the principle of centrifugal force-induced phase separation, it enables reproducible isolation of large cellular components—including nuclei, mitochondria, lysosomes, and viral particles—under precisely regulated thermal conditions. Its robust mechanical architecture integrates a high-torque AC brushless frequency-controlled motor with microprocessor-based closed-loop speed regulation, ensuring stable rotational dynamics across extended run durations. The unit maintains operational integrity within a wide temperature envelope (−20 °C to +40 °C), supported by an imported R404a refrigeration system compliant with international environmental standards for ozone depletion potential (ODP) and global warming potential (GWP). Designed for clinical diagnostics, biopharmaceutical process support, blood banking, and academic life science research, the DDL-5M meets foundational requirements for GLP-compliant sample preparation prior to downstream analytical techniques such as ELISA, Western blotting, nucleic acid extraction, and protein purification.

Key Features

• Microprocessor-controlled operation with programmable protocols, enabling storage of up to 10 user-defined methods including customizable acceleration/deceleration profiles.
• Intuitive touch-panel interface with dual digital display showing real-time RPM and RCF values, with automatic conversion between units during parameter entry.
• Integrated electronic door lock with multiple hardware-enforced safety interlocks: lid auto-locking mechanism, overspeed detection, imbalance sensing, overtemperature cutoff, and rotor recognition verification.
• Acoustic optimization yielding noise emission below 65 dB(A) at full load—critical for shared laboratory environments requiring low ambient disturbance.
• High-efficiency cooling system featuring a sealed, maintenance-free compressor and environmentally certified R404a refrigerant, delivering consistent thermal performance even under continuous 99-minute runs.
• Comprehensive fault diagnostics with audible and visual alerts, supporting rapid troubleshooting without service intervention.

Sample Compatibility & Compliance

The DDL-5M accommodates a broad spectrum of primary sample containers through interchangeable horizontal rotors, including conical tubes (7 mL, 15 mL, 20 mL, 50 mL), large-volume bottles (500 mL and 750 mL), and standardized microtiter plates (96-well formats). All rotors are dynamically balanced and CE-marked per EN 61000-6-2 (EMC immunity) and EN 61000-6-4 (EMC emissions). The system adheres to ISO 13485-aligned manufacturing practices and supports audit-ready documentation for laboratories operating under FDA 21 CFR Part 11–enabled data governance frameworks when paired with validated LIMS integration. Temperature control accuracy of ±1 °C ensures compliance with USP sterility testing pre-centrifugation conditioning and ASTM E2919-13 guidelines for cell pellet integrity preservation.

Software & Data Management

While the DDL-5M operates via embedded firmware without external PC dependency, its programmable memory retains all runtime parameters—including speed setpoint, duration, temperature target, ramp profiles, and rotor ID—for traceable method execution. Each run logs timestamped metadata (start/stop time, final RCF, achieved temperature stability window, error codes if triggered), accessible via front-panel recall. For laboratories implementing electronic record retention, optional RS-232 or USB-to-serial interfaces enable export of run logs into CSV-formatted files compatible with validated electronic lab notebooks (ELNs) and quality management systems (QMS). Audit trail functionality satisfies GLP and GMP Annex 11 prerequisites when combined with institutional procedural controls governing access rights and change management.

Applications

• Clinical hematology: Separation of whole blood into plasma, buffy coat, and erythrocyte fractions for coagulation assays and transfusion support.
• Virology and vaccine development: Harvesting enveloped viruses (e.g., influenza, SARS-CoV-2 pseudovirions) from clarified culture supernatants using low-shear 4000 rpm protocols.
• Biotherapeutics: Clarification of monoclonal antibody harvests prior to depth filtration and chromatographic purification steps.
• Environmental microbiology: Concentration of waterborne pathogens (Cryptosporidium, Giardia) from large-volume (750 mL) environmental samples.
• Cell biology: Isolation of intact organelles (nuclei, mitochondria) from tissue homogenates using differential centrifugation gradients.
• Immunology: Pellet recovery of antigen-antibody complexes in radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) workflows.

FAQ

What is the maximum relative centrifugal force (RCF) achievable with the DDL-5M?

The DDL-5M delivers a maximum RCF of 3780 × g at 5000 rpm with the 50 mL × 6 rotor configuration.
Does the DDL-5M support regulatory-compliant data integrity requirements?

Yes—when integrated with institutionally validated software layers and procedural controls, its parameter logging and method recall capabilities align with FDA 21 CFR Part 11 and EU Annex 11 expectations for electronic records.
Can the DDL-5M maintain −20 °C during extended runs?

It achieves and sustains −20 °C setpoints under standard ambient conditions (≤25 °C, ≤60% RH); performance may vary slightly under high-load, high-ambient-temperature scenarios.
Are rotor certifications included with purchase?

Each rotor is supplied with individual calibration certificates verifying dynamic balance and material compliance per ISO 21501-4 and IEC 61000-4-3 standards.
Is remote monitoring or network connectivity available?

The base model does not include Ethernet or Wi-Fi; however, serial output (RS-232) permits integration with centralized lab device management platforms via third-party middleware.