LuXiangYi DL-5M High-Capacity Refrigerated Floor-Standing Centrifuge

Overview

The LuXiangYi DL-5M High-Capacity Refrigerated Floor-Standing Centrifuge is engineered for reproducible, high-yield separation of temperature-sensitive biological samples across clinical, pharmaceutical, and academic laboratories. Designed around a robust horizontal rotor architecture and compliant with ISO 15195:2018 (clinical laboratory centrifuge performance requirements), the DL-5M operates on the principle of sedimentation under controlled gravitational force (RCF), enabling efficient pelleting of large cellular components—including nuclei, mitochondria, lysosomes, and enveloped viruses—without compromising structural integrity or functional activity. Its refrigerated operation maintains sample integrity during extended runs, particularly critical in applications involving plasma fractionation, vaccine formulation, monoclonal antibody purification, and cell culture harvest where thermal denaturation must be minimized. The unit integrates a high-torque AC brushless frequency-controlled motor and precision-engineered rotor dynamics to ensure rotational stability even at maximum load, delivering consistent RCF distribution across all tube positions.

Key Features

• Microprocessor-controlled system with intuitive touch-panel interface and multi-language support (English default), supporting up to 10 programmable protocols with automatic parameter recall.
• Imported high-efficiency compressor assembly utilizing eco-friendly R404a refrigerant, achieving stable temperature control from −20 °C to +40 °C with ±1 °C accuracy over full operational range.
• Real-time digital display showing simultaneous rpm and RCF values, with one-touch conversion between units—eliminating manual calculation errors during method transfer.
• Electronic lid lock with mechanical interlock ensures rotor containment during operation; integrated safety logic continuously monitors rotor balance, speed deviation, temperature drift, and power anomalies.
• Adjustable acceleration and deceleration profiles (10 pre-defined curves) allow optimization of shear-sensitive separations—e.g., minimizing membrane vesicle disruption during exosome isolation or preserving erythrocyte morphology in blood banking workflows.
• Low-noise design (<65 dB[A] at 1 m) meets ISO 15190:2022 ambient noise requirements for shared laboratory environments and GLP-compliant facilities.

Sample Compatibility & Compliance

The DL-5M accommodates diverse sample formats through interchangeable horizontal rotors certified to ISO 21501-4 for dimensional tolerance and dynamic balancing. Standard configurations include 4 × 1000 mL buckets (4000 rpm, 4100 × g), 6 × 300 mL carriers (5000 rpm, 3850 × g), and high-capacity microplate carriers (7 mL × 148, 4000 rpm, 3780 × g). All rotors are compatible with common polypropylene, polycarbonate, and glass centrifuge tubes meeting ASTM D4979 standards. The system complies with IEC 61010-1:2010 (safety requirements for electrical equipment) and IEC 61010-2-020:2016 (particular requirements for centrifuges), and supports audit-ready documentation for FDA 21 CFR Part 11–compliant environments when paired with optional data logging software.

Software & Data Management

While the DL-5M operates as a standalone instrument with embedded firmware, its RS-232 and optional USB interfaces enable seamless integration into LIMS or ELN platforms. Protocol parameters—including time, temperature, speed, acceleration/deceleration ramps, and rotor ID—are stored in non-volatile memory with timestamped revision history. Optional PC-based software provides CSV export of run logs, trend analysis of thermal performance, and electronic signature capability for SOP adherence in GMP-regulated bioprocessing labs. All data entries conform to ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) per WHO TRS 992 Annex 5 guidance.

Applications

• Clinical diagnostics: Serum/plasma separation from whole blood; isolation of PBMCs and platelet-rich plasma (PRP).
• Biopharmaceutical manufacturing: Harvest and clarification of CHO or HEK293 cell cultures prior to downstream chromatography.
• Virology research: Concentration of lentiviral or adenoviral vectors without loss of infectivity.
• Environmental microbiology: Sedimentation of activated sludge or algae biomass in water treatment studies.
• Blood banking: Large-volume red blood cell washing and buffy coat preparation under controlled thermal conditions.
• Academic core facilities: High-throughput processing of tissue homogenates for subcellular fractionation workflows aligned with NIH Common Fund initiatives.

FAQ

What is the maximum permissible imbalance tolerance for the 1000 mL rotor configuration?
The DL-5M’s horizontal rotor system is rated for ≤5 g mass imbalance per bucket pair, verified per ISO 15195 Annex C test procedures.
Can the centrifuge maintain −20 °C at maximum rotor speed and full load?
Yes—the refrigeration system is thermally oversized to sustain −20 °C setpoint at 4000 rpm with 4 × 1000 mL aqueous loads, validated per IEC 61010-2-020 Clause 7.3.2.
Is rotor calibration traceable to national metrology institutes?
Each rotor carries a unique serial number and factory calibration certificate traceable to CNAS-accredited reference standards (equivalent to NIST SRM 2822 for angular velocity verification).
Does the instrument support remote monitoring via Ethernet or Wi-Fi?
Ethernet connectivity is available via optional module (part no. DL-5M-ETH); Wi-Fi is not supported due to electromagnetic interference constraints in regulated lab environments.
Are rotor lifetime and maintenance intervals documented per ISO 21501-4?
Yes—rotor service life is defined as 10,000 cycles or 5 years (whichever occurs first), with mandatory visual inspection and balance verification every 500 cycles per manufacturer’s technical bulletin TB-DL5M-REV3.