LY DYCP-32B Agarose Horizontal Electrophoresis System (Medium Size)
| Brand | LY |
|---|---|
| Origin | Beijing, China |
| Model | DYCP-32B |
| Instrument Type | Nucleic Acid Electrophoresis Apparatus |
| Gel Tray Dimensions (L × W) | 200 × 130 mm, 150 × 130 mm |
| Overall Dimensions (L × W × H) | 365 × 165 × 130 mm |
| Buffer Capacity | 800 mL |
| Weight | 1.5 kg |
| Sample Comb Options | 14-, 26-, and 18-tooth combs |
| thicknesses | 1.0 mm and 1.5 mm |
| Material | Polycarbonate (injection-molded, leak-proof) |
| Design Features | Bridge-type electrode configuration, removable electrode assembly, transparent lid, integrated gel caster |
Overview
The LY DYCP-32B Agarose Horizontal Electrophoresis System is an engineered solution for routine nucleic acid separation in academic, clinical, and industrial molecular biology laboratories. Designed according to standard horizontal electrophoresis principles—where DNA fragments migrate through an agarose matrix under a uniform DC electric field—the system supports reliable visualization, sizing, and recovery of DNA bands ranging from ~100 bp to >20 kbp. Its medium-format architecture balances throughput and benchtop footprint, making it suitable for daily use in QC testing, PCR product verification, restriction digest analysis, and plasmid purification workflows. The unit operates without integrated power supply (requires external DC power source), conforming to IEC 61010-1 safety standards for laboratory electrical equipment.
Key Features
- Bridge-style electrode configuration minimizes buffer consumption while maintaining uniform field distribution across the gel surface—reducing run-to-run variability and thermal gradient effects.
- Injection-molded polycarbonate body ensures structural integrity, chemical resistance to common electrophoresis buffers (TAE, TBE, SB), and zero leakage under prolonged operation.
- Transparent, hinged upper lid enables real-time monitoring of electrophoretic migration without interrupting voltage application or compromising containment.
- Removable electrode assembly—including platinum-coated stainless steel electrodes and adjustable electrode holders—facilitates thorough cleaning, replacement, and maintenance per GLP-aligned lab practices.
- Integrated positioning stops on the gel tray prevent misalignment during comb insertion, ensuring consistent well geometry and reproducible loading volumes (compatible with 12-channel pipettes).
- High-flexibility, insulated leads with secure banana-jack terminations meet UL/CSA cable safety requirements and withstand repeated bending without conductor fatigue.
- Heat-resistant construction maintains dimensional stability at ambient temperatures up to 40 °C, eliminating warping during extended high-voltage runs.
Sample Compatibility & Compliance
The DYCP-32B accommodates dual gel tray formats (200 × 130 mm and 150 × 130 mm), supporting standard agarose concentrations (0.7–2.0%) and low-melt variants. Pre-cast or hand-poured gels are compatible, and the included gel caster enables rapid, bubble-free slab formation. Sample combs (14-, 26-, and 18-tooth; 1.0 mm and 1.5 mm thicknesses) allow optimization for resolution (narrow wells) or loading capacity (wider wells). The system complies with ISO 13485–aligned manufacturing controls and meets CE marking requirements for laboratory apparatus (2014/30/EU EMC Directive and 2014/35/EU LVD). While not a medical device, its design supports workflows aligned with CLIA, CAP, and ISO/IEC 17025 documentation expectations.
Software & Data Management
As a standalone electrophoresis platform, the DYCP-32B does not incorporate embedded firmware or digital interfaces. It functions as a passive, analog electrophoresis chamber—ensuring compatibility with any external DC power supply (e.g., EPS 300 series, PowerPac Basic) and downstream imaging systems (UV transilluminators, gel documentation stations). This architecture eliminates firmware validation overhead and supports audit-ready traceability when paired with calibrated power supplies logged via LIMS or electronic lab notebooks. No proprietary software is required; data capture occurs externally using standard image analysis tools (e.g., ImageJ, AlphaView SA) compliant with FDA 21 CFR Part 11 when deployed in regulated environments.
Applications
- Verification of PCR amplification products and RT-PCR outputs
- Analysis of restriction enzyme digestion patterns for cloning validation
- Size estimation of genomic DNA fragments and plasmid isoforms
- Quality control of purified nucleic acids prior to sequencing or transfection
- Educational demonstrations of charge-based macromolecular mobility
- Preparative isolation of DNA bands for excision and extraction
FAQ
Does the DYCP-32B include a built-in power supply?
No—it is a gel tank only and requires an external DC power supply capable of delivering 0–300 V and 0–500 mA.
Can this system be used for SDS-PAGE or protein electrophoresis?
No—it is optimized for nucleic acid separation in agarose matrices; polyacrylamide applications require vertical electrophoresis systems with different buffer dynamics and cooling requirements.
Is the polycarbonate housing resistant to UV exposure during post-run imaging?
Yes—grade-certified polycarbonate resists yellowing and embrittlement under standard 302 nm UV transillumination for durations typical of band excision protocols.
Are replacement combs and trays available separately?
Yes—LY provides full spare parts support under part numbers 141-3220 through 141-3224 and 143-3220 through 143-3221, with documented compatibility and dimensional tolerances.
What buffer volume is recommended for optimal performance?
A minimum of 600 mL is advised for full coverage of the 200 × 130 mm tray; the 800 mL total capacity allows margin for evaporation during extended runs at ≤150 V.
