LY DYCZ-20C DNA Sequencing Electrophoresis Apparatus

Overview

The LY DYCZ-20C DNA Sequencing Electrophoresis Apparatus is a high-stability vertical electrophoresis system engineered for high-resolution nucleic acid separation in molecular biology laboratories. Designed specifically for denaturing polyacrylamide gel electrophoresis (PAGE), it supports Sanger-based DNA sequencing, differential display PCR, single-strand conformation polymorphism (SSCP) analysis, and DNA fingerprinting applications. The apparatus operates on the principle of electrophoretic mobility under controlled electric field conditions—where DNA fragments migrate through a sieving matrix at rates inversely proportional to their logarithmic molecular weight. Its robust acrylic tank construction, precision-machined glass plate assembly, and optimized buffer circulation geometry ensure uniform field distribution and minimal thermal gradient across the gel surface—critical for reproducible band resolution and inter-run comparability.

Key Features

• Integrated vertical electrophoresis platform with modular upper and lower buffer chambers, enabling efficient heat dissipation and stable temperature maintenance during extended runs (e.g., overnight sequencing gels)
• Dual-glass plate assembly (2 concave + 2 flat plates) with engraved alignment marks and silicone gasket sealing—ensuring leak-free casting and consistent gel thickness (0.4 mm standard)
• High-capacity buffer reservoir (1500 mL total) with dedicated drain port for rapid buffer exchange and contamination control between experiments
• Ergonomic comb holder and pre-calibrated shark-tooth combs (68-tooth standard; 100-tooth optional) for uniform well formation and minimal sample diffusion during loading
• Stainless-steel electrode terminals with corrosion-resistant plating and low-resistance contact design—minimizing voltage drop and ensuring current stability across full operating range
• Compact footprint (410 × 210 × 430 mm) suitable for standard laboratory benchtops, with integrated leveling feet for operational stability

Sample Compatibility & Compliance

The DYCZ-20C accommodates standard 300 × 340 mm polyacrylamide or agarose gels, supporting acrylamide concentrations from 4% to 20% (w/v) and denaturants including urea (up to 8 M) or formamide. It is compatible with common nucleic acid stains (e.g., SYBR Gold, ethidium bromide, GelRed) and UV transillumination systems operating at 254 nm or 302 nm. While not an IVD device, the system conforms to general laboratory equipment safety standards per GB/T 29791.3–2013 (Chinese equivalent of IEC 61010-1) for electrical safety in laboratory environments. Its mechanical design supports GLP-aligned documentation practices when paired with validated power supplies (e.g., LY DYY-10C, DYY-12D, DYY-15D series) and calibrated pipetting workflows.

Software & Data Management

As a standalone electrophoresis apparatus, the DYCZ-20C does not include embedded firmware or digital interfaces. All operational parameters—including voltage, current, and run time—are externally controlled via compatible DC power supplies. For traceability in regulated environments, users are advised to record run conditions manually or integrate with LIMS-compatible power supply units that support audit-trail logging (e.g., models compliant with FDA 21 CFR Part 11 when used with electronic signature modules). Gel imaging data generated post-electrophoresis may be captured using third-party CCD or CMOS gel documentation systems and processed using open-source (ImageJ/Fiji) or commercial software (Bio-Rad Quantity One, TotalLab TL120), all of which accept standard TIFF or PNG outputs.

Applications

• DNA sequencing ladder resolution (e.g., dideoxy termination products up to 500 bp with sub-base-pair discrimination under optimized conditions)
• SSCP mutation screening in clinical research settings—detecting conformational changes induced by single-nucleotide variants
• Differential display profiling of cDNA populations across tissue types or treatment conditions
• Forensic DNA fingerprinting using multi-locus probe hybridization or PCR-amplified STR loci
• Verification of CRISPR-Cas9 editing efficiency via fragment size shift analysis in T7E1 or Surveyor nuclease assays
• Quality control of synthetic oligonucleotides and enzymatic digestion products prior to cloning or NGS library preparation

FAQ

What power supply models are compatible with the DYCZ-20C?
The DYCZ-20C is designed for use with LY’s DYY-series DC power supplies, including DYY-10C, DYY-12, DYY-12C, DYY-12D, and DYY-15D. These supplies provide adjustable constant-voltage or constant-current output up to 600 V / 500 mA.
Can the system be used for protein electrophoresis?
While primarily optimized for nucleic acids, the DYCZ-20C can accommodate SDS-PAGE gels when paired with appropriate combs and buffer systems; however, its 0.4 mm gel thickness and standard comb geometry are less ideal for high-molecular-weight protein separation compared to dedicated protein electrophoresis systems.
Is the glass plate set autoclavable?
No—the acrylic tank and silicone gaskets are not autoclave-rated. Glass plates may be sterilized via ethanol wipe or UV irradiation; chemical cleaning with mild detergent and deionized water is recommended for routine maintenance.
What is the maximum recommended run time at 200 V?
Under standard 6% denaturing PAGE conditions (8 M urea, 1× TBE), continuous operation up to 16 hours is feasible with adequate cooling (ambient lab temperature ≤25°C); longer runs require active cooling or voltage reduction to prevent gel overheating and band distortion.
Are replacement parts available internationally?
Yes—LY authorized distributors supply consumables and spare components globally, including shark-tooth combs (P/N 141-2031, 141-2033), silicone gaskets (P/N 143-2003), and glass plates (P/N 142-2031/142-2032) under standardized part numbering aligned with ISO 9001 traceability protocols.