LY DYCZ-24EN Dual Vertical Electrophoresis Apparatus (Medium Size)

Overview

The LY DYCZ-24EN Dual Vertical Electrophoresis Apparatus (Medium Size) is a precision-engineered system designed for high-resolution protein separation via vertical slab gel electrophoresis, with primary application in the second dimension of two-dimensional electrophoresis (2-DE) and isoelectric focusing (IEF). Built upon classical polyacrylamide gel electrophoresis (PAGE) principles, the instrument utilizes constant-voltage or constant-current power supply inputs (not included) to drive charged biomolecules through a homogeneous or gradient polyacrylamide matrix under controlled thermal and mechanical conditions. Its dual-gel configuration enables simultaneous processing of two independent 130 × 100 mm gels—enhancing throughput while maintaining inter-gel reproducibility critical for comparative proteomic analysis. The apparatus operates without external cooling but is fully compatible with external temperature control systems such as the WD-9412A constant-temperature circulating bath, supporting protocols requiring thermal stabilization during extended runs (e.g., IEF or high-percentage resolving gels).

Key Features

• Optically transparent polycarbonate housing and casting chamber—enabling real-time visual monitoring of gel polymerization and electrophoretic migration without disassembly.
• Integrated in-situ gel casting system: eliminates manual gel transfer, reduces interface defects, and ensures precise alignment between glass plates and sealing components.
• Zero-buffer-leak design achieved via dual-layer silicone gasketing and precision-machined acrylic frame geometry—prevents electrolyte loss and maintains stable conductance throughout runs.
• Flush-bottom gel configuration: the lower edge of the gel matrix aligns exactly with the bottom of the glass plates, eliminating air entrapment at the gel–buffer interface—a common source of band distortion in vertical systems.
• Modular comb insertion system accommodates interchangeable sample combs (12- and 16-well, 1.0 mm and 1.5 mm thickness), supporting diverse loading volumes and resolution requirements across SDS-PAGE, native PAGE, and IEF applications.
• Fail-safe electrical architecture: lid-open circuit interruption prevents accidental exposure to high voltage; flexible, abrasion-resistant leads ensure long-term reliability in high-use laboratory environments.
• Field-replaceable platinum-coated stainless steel electrodes—minimizing maintenance downtime and preserving consistent current distribution over extended service life.
• Compact footprint (210 × 120 × 220 mm) and lightweight construction (2 kg) facilitate integration into shared core facilities or mobile analytical workstations.

Sample Compatibility & Compliance

The DYCZ-24EN supports standard vertical polyacrylamide gel formats used in academic, clinical, and industrial proteomics workflows. It is routinely employed in protocols compliant with ISO/IEC 17025 for testing laboratories, and its mechanical consistency supports adherence to GLP documentation standards when paired with validated power supplies and staining/imaging systems. While the apparatus itself does not carry CE or FDA 510(k) certification, its materials (medical-grade polycarbonate, borosilicate glass, food-grade silicone) meet USP Class VI biocompatibility requirements for contact with biological samples. Buffer compatibility includes Tris-Glycine, Tris-Tricine, and IPG buffer systems—ensuring flexibility across first-dimension IEF and second-dimension SDS-PAGE workflows.

Software & Data Management

As a standalone electrophoresis platform, the DYCZ-24EN does not incorporate embedded firmware or digital controls. All operational parameters—including voltage, current, power, and run duration—are managed externally via certified electrophoresis power supplies (e.g., Bio-Rad PowerPac Basic, C.B.S. Scientific EPS-200). However, its mechanical stability and dimensional repeatability ensure high inter-run reproducibility—critical for downstream densitometric quantification using third-party image analysis software (e.g., ImageLab, AlphaView SA, or open-source tools such as Fiji/ImageJ). When integrated into validated laboratory information management systems (LIMS), the unit’s consistent physical specifications support audit-ready recordkeeping per FDA 21 CFR Part 11 requirements for electronic records and signatures.

Applications

• Second-dimension separation in two-dimensional electrophoresis (2-DE) following isoelectric focusing of complex protein extracts from cell lysates, tissue homogenates, or biofluids.
• High-resolution SDS-PAGE for molecular weight determination, purity assessment, and post-translational modification analysis.
• Native PAGE for functional protein complex characterization under non-denaturing conditions.
• Preparative-scale electrophoresis for gel excision and downstream mass spectrometry (MS) sample preparation.
• Teaching laboratories requiring robust, intuitive platforms for undergraduate and graduate instruction in protein biochemistry and molecular biology techniques.

FAQ

Is the DYCZ-24EN compatible with standard electrophoresis power supplies?

Yes—it accepts standard DC output terminals (typically 0–600 V, 0–500 mA) and conforms to universal electrode spacing and polarity conventions.
Can single-gel (monodirectional) runs be performed?

Yes—the system supports single-gel operation using the included 5 mm spacer glass plate and appropriate comb configuration.
What maintenance is required for long-term performance?

Routine cleaning of glass plates and silicone gaskets with deionized water and mild detergent; periodic inspection of electrode integrity and replacement every 12–24 months under continuous use.
Does the apparatus include a built-in cooling system?

No—it relies on ambient convection or optional external temperature regulation via the WD-9412A circulating bath or similar chillers.
Are replacement parts available globally?

Yes—LY authorized distributors maintain inventory of all consumables and wear components, including combs, glass plates, gaskets, and electrode assemblies, with documented traceability and lot-controlled packaging.