LY DYCZ-30D Dual-Plate Sandwich-Style Vertical Electrophoresis Apparatus
| Brand | LY |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Country of Origin | China |
| Model | DYCZ-30D |
| Instrument Type | Protein Electrophoresis Apparatus |
| Gel Dimensions (L × W) | 105 × 227 mm |
| Overall Dimensions (L × W × H) | 280 × 165 × 165 mm |
| Buffer Capacity | 2200 mL |
| Weight | 4 kg |
| Sample Combs | 31-tooth & 49-tooth, 1.0 mm & 1.5 mm thickness |
Overview
The LY DYCZ-30D Dual-Plate Sandwich-Style Vertical Electrophoresis Apparatus is a robust, benchtop protein electrophoresis system engineered for reproducible SDS-PAGE, native PAGE, and isoelectric focusing (IEF) applications in academic, clinical, and quality control laboratories. Its sandwich-style vertical gel casting architecture employs two parallel glass plates clamped with precision-machined spacers to form a uniform, leak-proof gel cassette—eliminating the need for sealing agents or time-consuming sealing steps. This design ensures consistent gel thickness across the entire active area (105 mm × 227 mm), critical for high-resolution band separation and inter-gel comparability. The apparatus operates under standard constant-voltage or constant-current power supply conditions (not included), supporting standard polyacrylamide gel electrophoresis protocols per ISO 13022, ASTM D7251, and USP <621>. Its compact footprint (280 × 165 × 165 mm) and low mass (4 kg) facilitate integration into shared lab spaces, biosafety cabinets, or mobile QC stations without compromising mechanical stability during extended runs.
Key Features
- Sandwich-style dual-glass-plate assembly with interchangeable 1.0 mm and 1.5 mm spacer frames—enabling rapid, repeatable gel casting without glue or agarose sealants.
- Integrated buffer chamber design with 2200 mL total capacity, optimized for thermal dissipation during high-voltage runs (up to 300 V typical); side-mounted buffer level indicators support visual monitoring without opening the unit.
- Modular comb system: Four comb types included—31-tooth and 49-tooth configurations, each available in both 1.0 mm and 1.5 mm thickness—supporting variable sample loading volumes (5–25 µL per well) and compatibility with multi-well pipetting.
- Corrosion-resistant anodized aluminum frame and stainless-steel electrode terminals ensure long-term durability in repeated exposure to Tris-Glycine, Tris-Tricine, and CAPS-based running buffers.
- Tool-free assembly/disassembly: Glass plates, spacers, and combs engage via precision alignment pins and spring-loaded clamps—achieving sub-0.1 mm parallelism tolerance without torque tools or calibration.
Sample Compatibility & Compliance
The DYCZ-30D accommodates standard acrylamide/bis-acrylamide gels (8–20% T, 2.6–3.3% C), gradient gels, and pre-cast compatible formats when used with optional adapter kits. It supports common protein staining workflows—including Coomassie Brilliant Blue R-250, silver nitrate, and fluorescent dyes (SYPRO Ruby)—without interference from leachable materials. All wetted components comply with ISO 10993-5 (cytotoxicity) and EU Directive 2002/72/EC for food-contact plastics (where applicable to polymer spacers). The system is routinely validated in GLP-compliant environments for seed purity testing (per ISTA Rule 5B) and biopharmaceutical QC release assays (aligned with ICH Q5E guidelines on comparability studies).
Software & Data Management
As a standalone electrophoresis apparatus, the DYCZ-30D does not incorporate embedded electronics or firmware. It is fully compatible with third-party imaging systems (e.g., Bio-Rad ChemiDoc, Azure Biosystems cSeries) and densitometry software (ImageLab, AlphaView SA, ImageJ/Fiji) via standard gel documentation workflows. For regulated environments, users may integrate the instrument into electronic lab notebook (ELN) systems using documented SOPs that capture gel composition, run parameters, and staining protocols—fully traceable under FDA 21 CFR Part 11 when paired with compliant software platforms and audit-trail-enabled hardware.
Applications
- Seed varietal purity assessment per national agricultural standards (e.g., NY/T 1432-2007, USDA-APHIS protocols).
- Protein molecular weight determination and purity verification in recombinant enzyme production.
- Stability-indicating assays for therapeutic proteins under accelerated degradation conditions (pH, temperature, oxidation).
- Teaching laboratory implementation of core electrophoretic principles—electrophoretic mobility, sieving effect, and charge-to-mass ratio dependence.
- Preparative-scale native PAGE for subsequent activity assays or mass spectrometry-compatible excision.
FAQ
Is the DYCZ-30D compatible with gradient gels?
Yes—the dual-plate architecture and uniform clamping pressure support stable gradient gel polymerization across the full 105 × 227 mm format when using standard gradient mixers and casting devices.
Can I use this unit for DNA electrophoresis?
While primarily optimized for protein separation, the apparatus can accommodate low-percentage agarose or composite gels for large DNA fragments (≥5 kb) when paired with appropriate running buffers and voltage settings; resolution below 500 bp is not recommended.
What power supply specifications are required?
A DC power supply delivering 0–300 V, 0–500 mA, and 0–125 W is recommended; constant-voltage mode is preferred for routine SDS-PAGE, while constant-current mode enhances reproducibility for native gels.
Are replacement parts available internationally?
Yes—LY-certified glass plates, spacers, combs, and sealing gaskets are distributed globally through authorized partners with documented lot traceability and QC certification (ISO 9001:2015).
Does the system meet CE or UL safety standards?
The DYCZ-30D is a passive electrophoresis chamber and carries no electrical components; compliance with IEC 61010-1 is achieved when operated with certified external power supplies meeting Class II insulation and overcurrent protection requirements.
