LY DYY-15D Computer-Controlled Triple-Constant Electrophoresis Power Supply
| Brand | LY (Beijing Liuyi) |
|---|---|
| Origin | Beijing, China |
| Model | DYY-15D |
| Output Channels | 4 parallel independent outputs |
| Voltage Range | 20–5000 V (2 V resolution) |
| Current Range | 3–300 mA (1 mA resolution) |
| Power Range | 5–300 W (1 W resolution) |
| Display | Large LCD showing real-time voltage, current, power, and timer |
| Programming | 10 stored protocols, each with up to 9 programmable steps |
| Operating Modes | Constant Voltage, Constant Current, Constant Power, Timer Mode, Volt-Hour Mode, Stepwise Program Mode |
| Safety Features | Overload protection, short-circuit auto-shutdown, over-temperature monitoring, low-current maintenance mode, power-fail recovery |
| Dimensions (W×D×H) | 278 × 349 × 110 mm |
| Weight | 4.0 kg |
Overview
The LY DYY-15D Computer-Controlled Triple-Constant Electrophoresis Power Supply is a precision-engineered instrument designed for rigorous molecular biology, clinical diagnostics, and pharmaceutical research laboratories requiring stable, programmable, and multi-parameter control of electrophoretic separations. Based on constant-voltage, constant-current, and constant-power regulation principles—collectively referred to as “triple-constant” operation—the DYY-15D delivers high reproducibility across diverse electrophoretic techniques including horizontal agarose gel electrophoresis, vertical SDS-PAGE, isoelectric focusing (IEF), and DNA sequencing gels. Its architecture supports simultaneous independent control of four output channels, enabling parallel experiments or multi-zone electrophoresis systems without cross-channel interference. The device complies with fundamental electrical safety standards for laboratory instrumentation (IEC 61010-1) and incorporates design features aligned with GLP-aligned workflow integrity, such as non-volatile protocol memory and power-fail recovery.
Key Features
- Triple-constant regulation: Independent real-time control of voltage (20–5000 V), current (3–300 mA), and power (5–300 W) ensures optimal field strength and thermal management for sensitive samples.
- Four isolated parallel DC outputs: Each channel operates independently with full parameter control—ideal for running multiple gels or segmented electrophoresis tanks under distinct conditions.
- Large backlit LCD interface: Simultaneously displays voltage, current, power, elapsed time, and remaining time—enabling immediate verification of operational parameters during run execution.
- Advanced programmability: Stores up to 10 user-defined protocols; each protocol supports up to 9 sequential steps with configurable mode (CV/CC/CP), setpoints, duration, and ramping behavior.
- Multi-mode operation: Supports standard constant-mode operation, timer-based termination, volt-hour integration (for IEF and native PAGE), and stepwise gradient programming for complex separation protocols.
- Robust safety architecture: Integrated microprocessor monitors internal temperature, output load, and circuit continuity—triggering automatic shutdown upon short-circuit detection, thermal overload, or sustained overcurrent conditions.
- Low-current maintenance mode: Maintains sub-mA output after main run completion to prevent gel diffusion while preserving sample integrity during post-run handling.
Sample Compatibility & Compliance
The DYY-15D is compatible with standard electrophoresis apparatuses used in academic, clinical, and industrial labs—including horizontal submarine units (e.g., for nucleic acid analysis), vertical mini- and maxi-gel systems (e.g., for protein separation), and specialized IEF trays. It supports common gel matrices (agarose, polyacrylamide, and composite gels) and buffer systems (TAE, TBE, Tris-Glycine, and ampholyte-based IEF buffers). While not certified to FDA 21 CFR Part 11 out-of-the-box, its deterministic program storage, non-volatile memory, and audit-ready parameter logging (via optional RS-232/USB data export) facilitate alignment with ISO/IEC 17025 and CLIA-compliant documentation practices. All electrical interfaces meet CE marking requirements for laboratory equipment safety and electromagnetic compatibility (EMC Directive 2014/30/EU).
Software & Data Management
The DYY-15D includes native RS-232 and USB-B interfaces for bidirectional communication with host PCs. Optional LY Electrophoresis Control Suite (v3.x) enables remote configuration, real-time parameter streaming, automated protocol deployment, and timestamped log file generation (.csv format) suitable for lab notebook integration. Exported data include voltage, current, power, time, and active mode—structured for traceability in QA/QC records. Firmware updates are delivered via secure USB flash drive, supporting version-controlled maintenance per institutional IT policies. No cloud connectivity or proprietary drivers are required; the device operates as a CDC-class serial peripheral under Windows, macOS, and Linux.
Applications
- Molecular biology: DNA fragment sizing (0.1–20 kb), plasmid analysis, PCR product verification, and restriction digest profiling.
- Protein characterization: SDS-PAGE for molecular weight determination, Western blot transfer optimization, and two-dimensional electrophoresis (2-DE) first-dimension IEF runs.
- Clinical diagnostics: Serum protein electrophoresis (SPEP), hemoglobin variant analysis, and monoclonal gammopathy screening.
- Biopharmaceutical development: Purity assessment of recombinant proteins, stability-indicating assays, and charge heterogeneity profiling.
- Education and training: Modular electrophoresis instruction in undergraduate and graduate teaching labs due to intuitive interface and fault-tolerant safety design.
FAQ
What electrophoresis techniques does the DYY-15D support?
It supports horizontal agarose electrophoresis, vertical SDS-PAGE, isoelectric focusing (IEF), pulse-field gel electrophoresis (PFGE) prep runs, and native PAGE—all via precise CV, CC, or CP regulation.
Can the DYY-15D be used with high-voltage applications like DNA sequencing gels?
Yes—its 5000 V maximum output and 2 V resolution enable accurate control for denaturing polyacrylamide sequencing gels requiring 1500–3000 V operation.
Does the device retain programmed methods after power loss?
Yes—non-volatile memory preserves all 10 protocols and system settings; power-fail recovery resumes the last active step upon re-energization.
Is calibration required, and how often?
Annual verification against NIST-traceable multimeters is recommended for ISO/IEC 17025 compliance; factory calibration certificates are provided with initial shipment.
How is thermal management handled during extended high-power runs?
An intelligent thermal sensor modulates fan speed and triggers protective shutdown if internal temperature exceeds 75 °C—ensuring long-term reliability without manual intervention.
