MKN SHA-2 Refrigerated Water Bath Orbital Shaker
| Brand | MKN |
|---|---|
| Model | SHA-2 |
| Type | Refrigerated Water Bath Orbital Shaker |
| Power Supply | 220 V, 50 Hz |
| Total Power | 2600 W |
| Timer Range | 0–999 min (digital display) |
| Oscillation Speed | 0–300 rpm (digital speed readout) |
| Oscillation Amplitude | 20 mm |
| Temperature Range | 5–100 °C (digital PID temperature control, full-range refrigeration & heating) |
| Max Load Capacity | 20 kg |
| Dimensions (W×D×H) | 685 × 460 × 560 mm |
| Origin | Jiangsu, China |
| Regulatory Status | CE-compliant design for laboratory use under IEC 61010-1 |
Overview
The MKN SHA-2 Refrigerated Water Bath Orbital Shaker is an integrated precision incubation platform engineered for controlled thermal and mechanical stimulation of biological and biochemical samples in liquid or semi-solid media. It operates on the principle of orbital shaking within a temperature-regulated water bath environment—combining Peltier-based active refrigeration with resistive heating and high-stability PID feedback control to maintain setpoint accuracy across the full 5–100 °C range. Unlike air-jacketed shakers, the water bath ensures uniform thermal distribution, minimal temperature gradients (<±0.3 °C at steady state), and superior heat transfer efficiency—critical for sensitive applications such as microbial culture expansion, enzyme kinetics assays, solubility studies, and cell suspension maintenance. Its robust stainless-steel water chamber, sealed motor drive assembly, and vibration-damped base structure deliver long-term operational stability under continuous load conditions up to 20 kg.
Key Features
- Full-range temperature control from 5 °C (below ambient) to 100 °C, enabled by dual-mode thermoelectric cooling and high-wattage heating elements (2600 W total power)
- Digital microprocessor controller with real-time display of both setpoint and actual temperature, plus independent digital speed readout (0–300 rpm, ±2 rpm repeatability)
- Orbital motion with fixed 20 mm amplitude—optimized for homogeneous mixing without shear-induced cell lysis or foam generation
- Programmable timer (0–999 minutes) with auto-shutdown and memory retention after power interruption
- Corrosion-resistant 304 stainless-steel water bath chamber with overflow protection and easy-drain valve
- Low-noise brushless DC motor coupled to a reinforced eccentric drive mechanism for extended service life and minimal maintenance
- Non-slip platform with universal clamping options accommodating standard flasks (50–5000 mL), test tubes, and multi-well plates
Sample Compatibility & Compliance
The SHA-2 supports a wide range of sample containers—including Erlenmeyer flasks, baffled flasks, serum bottles, and custom vessels—when used with compatible clamps or platforms. Its water bath design ensures consistent thermal coupling regardless of vessel geometry or fill volume, making it suitable for aerobic/anaerobic culture protocols per ISO 8601 and ASTM D2576 standards. The unit conforms to IEC 61010-1:2010 for electrical safety in laboratory equipment and meets EMC requirements per EN 61326-1. While not certified for GMP environments out-of-the-box, its digital logging capability (via optional RS-232/USB interface) supports audit-ready documentation aligned with GLP and FDA 21 CFR Part 11 when integrated into validated workflows.
Software & Data Management
The SHA-2 includes embedded firmware supporting local parameter storage (up to 10 user-defined programs), real-time clock synchronization, and password-protected settings lockout. Optional connectivity modules enable serial or USB data export to third-party LIMS or ELN systems. Time-stamped temperature and speed logs can be exported in CSV format for traceability, statistical process control (SPC), and regulatory review. Firmware updates are delivered via secure vendor portal; no cloud dependency or proprietary software installation is required.
Applications
- Microbial growth curve analysis and strain preservation at sub-ambient temperatures
- Thermally sensitive protein expression and purification workflows requiring precise cold-start agitation
- Accelerated stability testing of pharmaceutical formulations per ICH Q1A(R2)
- Biochemical reaction optimization—including ligand binding, immunoassay incubation, and antigen-antibody equilibration
- Environmental microbiology sampling preparation under standardized temperature-controlled agitation (e.g., EPA Method 1681)
- Cell line passaging and suspension culture maintenance for bioprocess development
FAQ
What is the minimum operating temperature, and does it require external coolant?
The unit achieves 5 °C without auxiliary chillers—its integrated thermoelectric module provides self-contained refrigeration down to 5 °C above ambient or below, depending on ambient conditions and load.
Can the SHA-2 accommodate stacked multi-layer platforms for high-throughput flask arrays?
Yes—optional accessory platforms with vertical spacing kits allow secure stacking of up to three tiers of 1-L flasks while maintaining thermal uniformity and mechanical stability.
Is temperature calibration traceable to NIST standards?
Factory calibration is performed using NIST-traceable RTD probes; users may perform field verification with external Class A PT100 sensors and adjust offset values via service menu.
Does the unit support remote monitoring via Ethernet or Wi-Fi?
No native Ethernet/Wi-Fi interface is included; however, RS-232 and USB-B ports support integration with industrial PLCs or PC-based SCADA systems using Modbus RTU or vendor-defined ASCII protocol.
What maintenance intervals are recommended for long-term reliability?
Biannual inspection of water bath seal integrity, quarterly cleaning of condenser fins (for refrigeration module), and annual verification of PID loop response time using step-change methodology per ISO/IEC 17025 guidelines.
