Nichiryo MODEL5100 Adjustable High-Capacity Manual Pipette

Overview

The Nichiryo MODEL5100 is a precision-engineered, manually operated single-channel pipette designed for high-volume liquid handling in analytical, quality control, and preparative laboratory workflows. Unlike conventional air-displacement pipettes, the MODEL5100 employs a positive-displacement principle with proprietary volume adjustment tubes—enabling accurate, repeatable dispensing across two discrete high-capacity ranges: 15–25 mL and 30–50 mL. Its robust stainless-steel body and ergonomic grip minimize operator fatigue during extended use, while its sealed piston mechanism ensures consistent performance with viscous, volatile, or temperature-sensitive liquids—including DMSO, glycerol, ethanol, and cell culture media—without reliance on air cushion compression. The device complies with ISO 8655-2:2022 (Pipettes — Part 2: Piston-operated volumetric apparatus) for mechanical design and functional verification.

Key Features

• Interchangeable volume adjustment tubes allow rapid reconfiguration between two calibrated high-volume ranges (15–25 mL and 30–50 mL), eliminating the need for multiple dedicated pipettes.
• Positive-displacement architecture minimizes evaporation, aerosol carryover, and calibration drift—critical for low-volatility solvents and biological suspensions.
• Accuracy certified to ±1.0% and coefficient of variation (CV) ≤1.0% at nominal volume, validated per ISO 8655-6:2022 (Testing and evaluation of performance).
• Compatible exclusively with Nichiryo TL50-series sterile, low-retention polypropylene tips (e.g., catalog no. 00-51TL50), supplied in 50-unit blister-packed configurations for traceability and sterility assurance.
• Tool-free tube exchange mechanism enables field recalibration without disassembly or service technician intervention—supporting GLP-compliant in-house verification protocols.
• Ergonomic, balanced weight distribution (approx. 380 g) and textured thumb rest reduce repetitive strain during serial dilution or bulk reagent preparation tasks.

Sample Compatibility & Compliance

The MODEL5100 is routinely deployed in applications requiring high-fidelity transfer of challenging samples: aqueous buffers, organic solvents, protein-rich lysates, and suspension-based formulations. Its positive-displacement system avoids under-delivery errors common with air-displacement pipettes when handling liquids with surface tensions <25 mN/m or densities outside 0.6–1.4 g/cm³. The instrument meets requirements for ISO/IEC 17025-accredited laboratories performing method validation under ICH Q2(R2); it supports documented traceability via tip lot numbers and user-maintained calibration logs. While not electronically connected, its mechanical design permits integration into audit-ready workflows compliant with FDA 21 CFR Part 11 when paired with paper-based or LIMS-linked calibration records.

Software & Data Management

As a fully manual, non-electronic instrument, the MODEL5100 does not incorporate embedded firmware, connectivity, or onboard data storage. Calibration and performance verification are conducted using external gravimetric methods (e.g., ASTM E542-21, “Standard Practice for Calibration of Laboratory Volumetric Apparatus”) and recorded in laboratory notebooks or electronic lab notebooks (ELNs) meeting ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). Nichiryo provides a downloadable calibration template aligned with ISO/IEC 17025 Annex A.3, supporting interval-based verification (recommended every 6 months or after 10,000 actuations).

Applications

• Preparation of large-volume standard solutions for HPLC, GC, and ICP-MS calibration curves.
• Transfer of cell culture media, transfection reagents, and cryopreservation solutions in biomanufacturing cleanrooms (ISO Class 5–7).
• Bulk dispensing of buffer stocks in molecular biology core facilities—particularly where repeated accuracy across 30–50 mL volumes is required for qPCR master mix preparation.
• QC sampling of pharmaceutical intermediates and excipients in GMP environments, where volumetric consistency directly impacts assay robustness.
• Environmental testing labs performing EPA Method 525.3 (purge-and-trap GC/MS) sample extraction with internal standard spiking at ≥25 mL volumes.

FAQ

Is the MODEL5100 compatible with third-party tips?
No. Only Nichiryo TL50-series tips (e.g., 00-51TL50) ensure proper piston seal engagement and volumetric fidelity. Non-OEM tips may cause leakage, inaccurate delivery, or piston wear.
Can the pipette be calibrated for volumes outside 15–25 mL or 30–50 mL?
Custom volume adjustment tubes can be ordered directly from Nichiryo Japan for ranges within 15–50 mL; however, factory certification applies only to the two standard configurations listed in the ISO 8655 test report.
What maintenance is required?
Routine cleaning with 70% ethanol and visual inspection of the piston O-ring every 500 uses; replacement O-rings (part no. 00-51OR10) are available through authorized distributors.
Does the MODEL5100 meet USP <1058> guidelines for analytical instrument qualification?
Yes—it satisfies the “Category C” (mechanical, non-automated) classification under USP <1058>; IQ/OQ documentation templates are provided with each unit.