Nichiryo Nichipet EX Plus II Single-Channel Chemical-Resistant Adjustable Micropipette

Overview

The Nichiryo Nichipet EX Plus II is a precision-engineered single-channel manual pipette designed for laboratories requiring reliable liquid handling in aggressive chemical environments. Built upon Nichiryo’s decades of Japanese engineering heritage, this pipette employs a fundamental air-displacement principle with enhanced material science to ensure long-term metrological stability under repeated exposure to corrosive reagents. Unlike standard pipettes whose internal components degrade when exposed to acetone, chloroform, concentrated HCl, NaOH, or ethyl acetate, the EX Plus II integrates fluoropolymer-sealed mechanisms, low-swelling fluoroelastomer (FKM) O-rings, and chemically inert ceramic pistons (for volumes ≥100 µL) to maintain volumetric accuracy and mechanical integrity across thousands of pipetting cycles. Its design conforms to ISO 8655-2:2022 for piston-operated volumetric apparatus, supporting traceable calibration and routine verification in GLP- and GMP-regulated workflows.

Key Features

• Full-assembly autoclavability at 121°C for 20 minutes—no disassembly required—ensuring sterility without compromising seal integrity or calibration.
• UV-C sterilization compatibility (254 nm), enabling rapid decontamination between hazardous sample runs.
• Two-stage dispensing mechanism: precise low-volume ejection (e.g., 2 µL or 10 µL) followed by full blow-out, minimizing residual carryover and improving reproducibility in viscous or volatile solvents.
• Integrated one-handed locking dial for volume adjustment—eliminates accidental setting drift during operation and supports rapid recalibration in situ.
• Corrosion-resistant alloy spring and PTFE-coated internal air pathways prevent solvent-induced metal fatigue and gas-path degradation.
• Digital volume display with tactile feedback and parallax-free readout, calibrated per ISO 8655-6 for readability and repeatability.

Sample Compatibility & Compliance

The Nichipet EX Plus II is validated for use with a broad spectrum of laboratory solvents, including but not limited to hydrochloric acid (up to 6 M), sodium hydroxide (up to 2 M), acetonitrile, methanol, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, chloroform, formaldehyde, and diethyl ether. Its FKM O-rings exhibit <1% volumetric swelling after 72-hour immersion in these agents (per ASTM D1414), while the ceramic piston maintains dimensional stability under thermal cycling (−20°C to +50°C). The device complies with ISO 8655 (Parts 1–7) for specification, testing, and calibration of piston pipettes and meets essential requirements of the EU Medical Device Regulation (MDR 2017/745) Annex I for Class I non-sterile instruments. Documentation includes factory calibration certificate traceable to NMIJ/AIST (Japan) standards.

Software & Data Management

As a manually operated, non-electronic pipette, the Nichipet EX Plus II does not incorporate embedded firmware or digital connectivity. However, its mechanical design supports full integration into regulated data ecosystems: calibration records—including date, operator ID, reference standard used (e.g., gravimetric traceable to NIST SRM 3190), and deviation values—can be documented in paper-based or electronic lab notebooks (ELN) compliant with FDA 21 CFR Part 11 when paired with audit-trail-enabled systems. Optional calibration verification kits (e.g., Nichiryo NP-CK2) provide certified weights and temperature-controlled water for on-site performance checks aligned with USP and ISO/IEC 17025 requirements.

Applications

This pipette is routinely deployed in analytical chemistry labs performing sample preparation for GC-MS and LC-MS analysis where solvent purity and instrument protection are critical; in pharmaceutical QC/QA for dissolution testing involving organic media; in polymer synthesis labs handling monomers and catalysts; and in environmental testing laboratories processing acidic digests (EPA Method 3050B) or alkaline extractions. Its robustness makes it especially suitable for high-throughput cytotoxicity assays using DMSO stock solutions, enzymatic reaction setups with glycerol-rich buffers, and forensic toxicology workflows involving volatile organic compounds.

FAQ

Can the Nichipet EX Plus II be calibrated in-house without specialized tools?
Yes—its front-mounted locking dial allows full-range recalibration using only a certified balance, distilled water, and ambient temperature monitoring per ISO 8655-6 procedures.
Is tip ejection force adjustable?
No—the ejection mechanism is optimized for BMT2-series tips; consistent mechanical resistance ensures uniform tip release without user variability.
Does the 0.1 µL minimum volume apply across all models?
No—the 0.1 µL lower limit applies only to the 0.1–2 µL variant (catalog #00-NPLO2-2); other variants have model-specific minimums as listed in the technical matrix.
Are replacement parts available globally?
Yes—Nichiryo distributes OEM seals, pistons, springs, and tip ejectors through authorized distributors in >60 countries, with lead times under 10 business days for most components.
How often should recalibration be performed?
Per ISO 8655-5, initial calibration is recommended before first use; thereafter, frequency depends on usage intensity and risk assessment—typically every 3–6 months for daily use in regulated environments.