PerkinElmer Flexar HPLC System (Refurbished)

Overview

The PerkinElmer Flexar HPLC System is a fully integrated, high-performance liquid chromatography platform engineered for precision, robustness, and operational continuity in regulated and research environments. Based on proven dual-piston reciprocating pump architecture and low-dead-volume fluidic design, the Flexar system delivers stable gradient formation, precise flow control (0.001–10.0 mL/min), and exceptional retention time reproducibility (<0.15% RSD). Its modular configuration—comprising a quaternary solvent manager with integrated degasser, refrigerated column oven (5–85°C), autosampler with needle-wash capability, and variable-wavelength UV/Vis detector (190–900 nm)—supports isocratic and gradient elution across diverse sample matrices. This refurbished unit has undergone comprehensive functional validation at SpectraLab Scientific’s ISO/IEC 17025-accredited refurbishment facility, including pressure testing, baseline noise assessment (<±0.5 × 10⁻⁵ AU), wavelength accuracy verification (±1 nm), and system suitability testing per USP . All hardware components are original PerkinElmer OEM parts; no third-party substitutions are used.

Key Features

• Quaternary solvent delivery system with real-time pressure monitoring and automatic leak detection
• Autosampler with 100-position vial capacity, programmable injection volume (0.1–100 µL), and integrated needle wash station to minimize carryover
• Column oven with Peltier-based cooling (5–85°C, ±0.2°C stability) and dual-zone temperature control for column and detector flow cell
• UV/Vis detector featuring dual-beam optics, deuterium/tungsten lamp source, and configurable slit width (1–8 nm) for optimized signal-to-noise ratio
• Intuitive Chromera Chromatography Data System (CDS) software with GLP-compliant audit trail, electronic signatures, and 21 CFR Part 11 compliance modules
• Modular “plug-and-play” fluidic architecture with color-coded tubing, standardized fittings (1/16″ SS), and pre-validated leak-tight connections
• Integrated solvent degasser with four-channel vacuum membrane technology and automatic pressure regulation

Sample Compatibility & Compliance

The Flexar HPLC accommodates a broad range of sample types—including small-molecule pharmaceuticals, natural product extracts, environmental contaminants, food additives, and polymer oligomers—across reversed-phase, normal-phase, ion-exchange, and size-exclusion modes. It supports standard 2.1–4.6 mm ID analytical columns (up to 250 mm length) and is compatible with UHPLC-ready hardware upgrades (e.g., low-dispersion detector flow cells, microbore tubing kits). All refurbished units meet ASTM E2689 (Standard Practice for Verification of HPLC Systems) and are validated against USP system suitability criteria (tailing factor ≤2.0, resolution ≥2.0, %RSD of retention time ≤1.0%). Documentation includes full calibration certificates, preventive maintenance logs, and a traceable refurbishment report signed by a certified LC applications engineer.

Software & Data Management

Chromera CDS v2.5 or later is pre-installed on a dedicated Windows 10 IoT Enterprise workstation with 16 GB RAM and SSD storage. The software provides method development tools (gradient optimization wizard, peak tracking algorithms), automated system suitability evaluation, and batch processing with customizable reporting templates (PDF, CSV, XML). Audit trail functionality records all user actions—including method edits, sequence modifications, and data reprocessing—with immutable timestamps and operator authentication. Data integrity is ensured via encrypted local storage, scheduled backups to network drives, and optional integration with LIMS platforms via ASTM E1384-compliant interfaces. Electronic signatures comply with FDA 21 CFR Part 11 requirements for regulated laboratories conducting QC/QA, stability studies, or method validation.

Applications

This Flexar system is routinely deployed in pharmaceutical quality control labs for assay quantitation (e.g., API purity per ICH Q2(R2)), dissolution testing, and impurity profiling. In academic and contract research settings, it supports metabolomics workflows (hydrophilic interaction chromatography coupled to MS), pesticide residue analysis (AOAC 2007.01), and polymer molecular weight distribution (GPC/SEC with multi-angle light scattering). Environmental labs utilize its gradient robustness for EPA Method 8330B (phthalates) and Method 549.2 (pesticides in drinking water). The system’s thermal stability and low baseline drift enable extended unattended runs—ideal for overnight stability-indicating methods or high-throughput screening assays requiring >100 injections per day.

FAQ

Is this a factory-refurbished unit or dealer-refurbished?
This system was refurbished by SpectraLab Scientific’s in-house engineering team following PerkinElmer-recommended procedures and ISO/IEC 17025-aligned validation protocols—not by a third-party vendor.
Does the warranty cover both hardware and software support?
Yes—the 6-month comprehensive warranty includes hardware replacement, firmware updates, Chromera software licensing, and remote technical assistance from certified LC specialists.
Can I upgrade to a fluorescence or mass spectrometric detector later?
Absolutely. The Flexar platform features standardized analog/digital I/O ports and open communication protocols (RS-232, Ethernet) compatible with PerkinElmer LS-55 fluorescence detectors and AxION® iQT mass spectrometers.
Are original consumables and spare parts included?
A starter kit containing 200 µL sample loops, 0.2 µm inline filters, column frits, and pump seal kits is provided. Full OEM part numbers and sourcing guidance are included in the delivery documentation.
How is system performance verified prior to shipment?
Each unit undergoes 72 hours of continuous operation under load, followed by USP system suitability testing using caffeine/acetaminophen standards, pressure cycling validation, and detector linearity assessment across 0.01–2.0 AU range.