PerkinElmer Lambda 2 UV-Vis Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda 2 UV-Vis Spectrophotometer is a double-beam, scanning spectrophotometer engineered for high reproducibility and long-term stability in routine quantitative and qualitative analysis across academic, pharmaceutical, and industrial quality control laboratories. Based on a Czerny-Turner monochromator design with a holographic grating and low-noise silicon photodiode detector, the Lambda 2 delivers reliable absorbance, transmittance, and reflectance measurements from 190 nm to 1100 nm. Its double-beam architecture continuously corrects for source drift and solvent background fluctuations—critical for extended kinetic studies or multi-sample batch analysis under ambient laboratory conditions. As a refurbished unit, this instrument has undergone full functional validation at SpectraLab Scientific’s ISO 9001-certified refurbishment facility, including wavelength accuracy verification (traceable to NIST SRM 2031 and 2034), photometric linearity assessment (using neutral density filters and potassium dichromate solutions), and baseline stability evaluation over 60 minutes.

Key Features

• Double-beam optical design with real-time reference compensation for enhanced measurement robustness
• Scanning speed up to 2,400 nm/min with programmable scan modes (continuous, step-scan, fixed-wavelength)
• Baseline flatness ≤ ±0.001 A over 200–1000 nm after auto-zero calibration
• Photometric accuracy: ±0.005 A at 0.5 A (verified with certified K₂Cr₂O₇ standard in 0.005 M H₂SO₄ per ASTM E275)
• Wavelength repeatability: ±0.1 nm; resolution: <1.0 nm (FWHM at 2 nm slit width)
• Integrated 6-position automatic sample changer compatible with 10 mm pathlength cuvettes (quartz or UV-grade fused silica)
• Front-panel keypad and LCD display enable standalone operation without PC dependency
• RS-232 interface supports legacy data export to LIMS or spreadsheet environments

Sample Compatibility & Compliance

The Lambda 2 accommodates standard 10 mm square cuvettes (including matched quartz pairs for high-precision work), as well as optional accessories such as fiber-optic probes, integrating spheres (for diffuse reflectance), and temperature-controlled holders (−10 °C to +100 °C with external bath). All refurbished units conform to original PerkinElmer factory specifications for stray light (<0.05% T at 220 nm using sodium iodide solution), noise level (≤0.0004 A RMS at 500 nm, 1 nm bandwidth), and photometric range (−0.3 to 3.0 A). Documentation includes a Certificate of Reconditioning, traceable calibration records, and a Statement of Conformance to ISO/IEC 17025:2017 requirements for testing laboratories. The system supports GLP-compliant workflows through manual audit trail logging and meets foundational criteria for FDA 21 CFR Part 11 readiness when paired with validated third-party data acquisition software.

Software & Data Management

While the Lambda 2 operates independently via its embedded firmware, it is fully compatible with PerkinElmer’s legacy UV WinLab™ v3.0 software (Windows XP/Vista/7 compatible) for advanced spectral processing—including derivative spectroscopy, multi-component quantitation, kinetics modeling, and report generation with customizable templates. Raw data exports in ASCII (.txt) or JCAMP-DX format ensure interoperability with MATLAB, OriginLab, or Python-based analysis pipelines. For modern lab informatics integration, the RS-232 port allows scripted control via LabVIEW or custom Python serial protocols. All refurbished systems ship with installation media, license keys (where applicable), and a complete set of user manuals—digitally archived and provided on USB drive.

Applications

This instrument is routinely deployed in applications requiring regulatory-grade optical density measurements: UV-Vis quantification of nucleic acids and proteins (A260/A280 ratios), dissolution testing of solid oral dosage forms (per USP <711>), colorimetric enzyme assays (e.g., ELISA endpoint detection), impurity profiling in small-molecule APIs, and polymer characterization via carbonyl index calculation. Its stability and low stray light make it suitable for measuring highly absorbing samples (e.g., concentrated dyes or transition metal complexes) without signal saturation artifacts. Academic users leverage its kinetic mode for monitoring reaction progress in real time—such as hydrolysis rates or redox equilibria—under controlled temperature and pH conditions.

FAQ

Is this Lambda 2 unit covered by original PerkinElmer warranty?

No—this is a refurbished instrument backed by SpectraLab Scientific’s 6-month limited hardware warranty covering defects in materials and workmanship.
Does the system include a computer and software?

The instrument does not include a host PC; UV WinLab™ software and necessary drivers are supplied on USB media and require a Windows-compatible laptop/desktop for full functionality.
Can I use non-PerkinElmer cuvettes?

Yes—standard 10 mm pathlength cuvettes from reputable suppliers (e.g., Hellma, Starna) are fully compatible, provided they meet dimensional tolerances and UV transmission specifications.
What documentation accompanies the shipment?

Each unit ships with a Certificate of Reconditioning, calibration verification report, operational checklist, safety manual, and full set of technical schematics and service bulletins.
Is remote support available after purchase?

Yes—SpectraLab Scientific provides complimentary email-based technical support for 90 days post-delivery, including troubleshooting guidance and method optimization recommendations.