PerkinElmer Lambda 25 UV-Vis Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda 25 UV-Vis Spectrophotometer is a dual-beam, double-monochromator spectrophotometer engineered for high-precision absorbance, transmittance, and reflectance measurements across the ultraviolet and visible spectral range (190–1100 nm). Its optical architecture employs holographic gratings and a solid-state detector (SCD), enabling superior photometric linearity, low stray light (<0.0003% at 220 nm), and exceptional baseline stability—critical for kinetic assays, trace-level quantitation, and regulatory-compliant QC workflows. Unlike photomultiplier tube (PMT)-based systems limited to ~900 nm, the SCD delivers uniform quantum efficiency across the full range, supporting applications from nucleic acid quantification to semiconductor thin-film characterization. As a refurbished unit sourced and validated by SpectraLab Scientific, this Lambda 25 has undergone full functional verification—including wavelength accuracy calibration (NIST-traceable standards), photometric linearity assessment, and baseline drift evaluation—ensuring metrological integrity equivalent to factory specifications.

Key Features

• Dual-beam, double-monochromator design with holographic gratings for minimized stray light and enhanced spectral resolution
• Fixed 1 nm slit width optimized for balance between signal-to-noise ratio and spectral fidelity in routine analysis
• Solid-state detector (SCD) providing extended spectral response (190–1100 nm), long-term stability, and immunity to high-intensity UV exposure
• Thermally stabilized optical bench and precision stepper-motor-driven wavelength drive for <±0.1 nm wavelength repeatability
• Integrated cuvette autoloader interface (compatible with AS93 Plus) and standardized accessory ports for seamless integration of temperature-controlled holders, integrating spheres, and fiber-optic probes
• Compliance-ready firmware supporting audit trail generation, user access control, and electronic signature functionality per FDA 21 CFR Part 11 when operated with UV WinLab in secure mode

Sample Compatibility & Compliance

The Lambda 25 accommodates standard 10 mm pathlength quartz, glass, or plastic cuvettes, as well as microvolume adapters (e.g., 1–5 µL capillary cells), solid-sample holders, and diffuse reflectance accessories including 50 mm integrating spheres (Lambda 35/45 compatible via adapter). It meets core performance criteria defined in ASTM E275 (Standard Practice for Describing and Measuring Performance of UV-Vis Spectrophotometers), ISO 6425 (Spectrophotometers — Performance Requirements), and USP (Spectrophotometry and Light-Scattering). All refurbished units undergo verification against these standards using certified reference materials (e.g., Holmium Oxide, Didymium filters, NIST SRM 930e). Documentation includes a Certificate of Verification detailing wavelength accuracy, photometric accuracy (±0.003 A at 0.5 A), and baseline noise (<0.0002 A RMS, 200–800 nm, 1 nm bandwidth).

Software & Data Management

Controlled exclusively via PerkinElmer’s UV WinLab v6.x software suite, the Lambda 25 supports method-driven operation with embedded validation protocols. Core capabilities include spectral acquisition, multi-wavelength quantitative analysis (single/multi-point calibration), kinetics modeling, and derivative spectroscopy. Optional modules extend functionality: KinLab enables enzyme kinetic parameter derivation (V_max, K_m) with real-time curve fitting; BioMethods Library provides pre-validated protocols for protein A₂₈₀, DNA A₂₆₀/A₂₈₀, and Bradford/Lowry assays. Data files (.spc) are stored in vendor-neutral format with embedded metadata (operator ID, timestamp, instrument serial, calibration history). Audit trails record all parameter changes, method executions, and report generations—fully exportable for GLP/GMP documentation review.

Applications

This instrument serves laboratories requiring robust, repeatable UV-Vis data under regulated or research-intensive conditions. Typical use cases include: quantification of biomolecules in biopharma QC (e.g., monoclonal antibody concentration, endotoxin-free buffer verification); dissolution testing support via absorbance monitoring at fixed wavelengths; thin-film thickness and bandgap analysis in materials science; colorimetric assay validation (ELISA, phosphatase activity); and environmental water quality screening (nitrate, phosphate, COD). Its thermal stability and low noise floor make it suitable for long-duration kinetic experiments (>2 hours) without baseline drift correction.

FAQ

Is this Lambda 25 fully calibrated and verified prior to shipment?

Yes. Each unit undergoes comprehensive performance verification using NIST-traceable standards, including wavelength accuracy, photometric linearity, stray light, and baseline stability. A full Certificate of Verification is provided.
Does the 6-month warranty cover parts, labor, and on-site service?

The warranty covers parts and labor for defects in materials or workmanship. Remote diagnostics and software support are included; on-site service is available at additional cost depending on location.
Can UV WinLab software be upgraded to latest version?

Yes. Units ship with licensed UV WinLab v6.x. Customers may purchase optional upgrade paths to v7.x through PerkinElmer’s authorized channels, subject to license transfer eligibility.
Are original accessories (e.g., cuvette holders, lamp housings) included?

All standard accessories present at time of refurbishment are included—typically: dual-cell holder, quartz cuvettes (10 mm), deuterium and tungsten lamps, and power/data cables. Optional accessories (e.g., Peltier temperature controller) are listed separately.
Is the instrument compliant with FDA 21 CFR Part 11 requirements?

When configured with UV WinLab in secure mode (user roles, electronic signatures, audit trail enabled), the system satisfies technical controls required for Part 11 compliance. Full validation documentation packages are available upon request.