PerkinElmer Lambda 35 UV-Vis Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda 35 UV-Vis Spectrophotometer is a dual-beam, double-monochromator spectrophotometer engineered for high-precision absorbance, transmittance, and reflectance measurements across the ultraviolet and visible spectral range (190–1100 nm). Its optical architecture employs holographic gratings and a solid-state detector (SCD), delivering superior stray light rejection (<0.0001% at 220 nm), extended photometric linearity (up to 3.5 AU), and exceptional baseline stability—critical for kinetic assays, long-duration scans, and regulatory-compliant workflows. Unlike PMT-based systems limited to ~900 nm, the SCD ensures uniform quantum efficiency and low noise across the full range, enabling reliable quantification of low-concentration analytes and high-absorbance samples without detector switching or gain adjustment.

Key Features

• Dual-beam, double-monochromator design with independently adjustable slit widths (0.5, 1, 2, or 4 nm) for optimized resolution/sensitivity trade-offs per application
• Holographic grating optics minimizing stray light and maximizing photometric accuracy—validated per ASTM E275 and ISO 6233
• Thermally stabilized solid-state detector (SCD) with 190–1100 nm spectral response, no warm-up delay, and >100,000-hour operational lifetime
• Integrated wavelength calibration using holmium oxide and didymium filters; auto-validation supported via UV WinLab software
• Modular accessory interface supporting temperature-controlled cuvette holders (Peltier or water-jacketed), integrating spheres (50 mm, Lambda 35-specific), fixed-angle (6°) and variable-angle (15–70°) specular reflectance stages, and fiber-optic probes with UV-grade transmission
• Rugged mechanical design with precision-ground optical mounts and sealed optical bench—engineered for reproducible performance under routine laboratory conditions

Sample Compatibility & Compliance

The Lambda 35 accommodates standard 10-mm pathlength quartz, glass, or plastic cuvettes, as well as microvolume cells, flow cells, and solid-sample stages. Its 50-mm integrating sphere enables quantitative diffuse reflectance analysis of powders, films, and coatings per ASTM E1331 and ISO 7724. When configured with AS93 Plus autosampler or dissolution accessories, it supports USP and compliance for pharmaceutical dissolution testing. Data integrity meets FDA 21 CFR Part 11 requirements when operated with UV WinLab’s audit-trail-enabled configuration—including electronic signatures, user-level access control, and immutable method/data archiving.

Software & Data Management

Controlled exclusively via PerkinElmer’s UV WinLab platform (v6.3.5 or later), the instrument supports method development, spectral acquisition, multi-point quantitation, kinetics modeling, and spectral library matching. Optional modules include KinLab for enzyme kinetics (Michaelis-Menten, Lineweaver-Burk), BioAssay for pre-validated biochemical protocols (n = 50+), and Spectrum Manager for centralized fleet monitoring. All raw data are stored in vendor-neutral .spc format; export to CSV, ASCII, or JCAMP-DX is fully scriptable. Software installations undergo IQ/OQ verification per GMP/GLP guidelines, with full documentation provided upon request.

Applications

• Pharmaceutical QC: Active pharmaceutical ingredient (API) assay, excipient identification, cleaning validation swab analysis
• Academic research: Ligand-binding studies, nanoparticle size estimation (via plasmon resonance), DNA/RNA quantification (A260/A280 ratio)
• Materials science: Thin-film thickness determination, bandgap calculation from Tauc plots, pigment concentration profiling
• Environmental testing: Nitrate/nitrite quantification in water, COD analysis via dichromate reduction, heavy metal chelation assays
• Food & beverage: Color index measurement (CIE L*a*b*), antioxidant capacity (FRAP, DPPH), preservative concentration monitoring

FAQ

Is this unit factory-refurbished or third-party refurbished?
This Lambda 35 has undergone full functional refurbishment by SpectraLab Scientific, including optical alignment verification, wavelength accuracy recalibration, photometric linearity validation, and full system stress testing per PerkinElmer service protocols.
Does the 6-month warranty cover parts, labor, and on-site service?
Yes—the warranty includes all components, labor, and remote technical support. On-site service is available in North America and EU regions under optional extended coverage plans.
Can the instrument be upgraded to support GLP-compliant electronic signatures?
Yes—UV WinLab v6.3.5+ with the Security Pack module satisfies 21 CFR Part 11 requirements for role-based access, audit trails, and electronic signature capture.
Are original accessories included (e.g., cuvette holder, calibration filters)?
All standard accessories shipped with the original Lambda 35 configuration are included unless otherwise specified in the quotation, along with Ho/Didy calibration filters and a traceable NIST-certified absorbance standard.
What documentation is provided with the instrument?
You will receive a Certificate of Refurbishment, full test reports (wavelength accuracy, photometric linearity, stray light), software license keys, and digital copies of the operator manual, service manual, and compliance validation templates.