PerkinElmer Lambda 850 UV/Vis/NIR Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda 850 UV/Vis/NIR Spectrophotometer is a high-performance, dual-beam, dual-monochromator ratio spectrophotometer engineered for precision quantitative and qualitative analysis across the ultraviolet, visible, and near-infrared spectral regions (175–900 nm). Its optical architecture incorporates aerospace-grade components—including holographic SiO₂-coated gratings (1440 lines/mm for UV/Vis, 360 lines/mm for NIR), a four-segment chopper enabling independent black-level correction for both sample and reference beams, and pre-aligned, auto-switching deuterium and tungsten-halogen lamps. This configuration delivers exceptional wavelength accuracy (±0.08 nm in UV/Vis), ultra-low stray light (<0.00008% T), and baseline flatness of ±0.0004 A over the full spectral range—critical for high-dynamic-range measurements such as thin-film transmittance, highly absorbing biological samples, or low-reflectance optical coatings.

Key Features

• Dual-monochromator design with double-pass Littrow optics ensures superior stray light rejection and spectral purity, meeting stringent requirements for pharmaceutical assay validation and materials characterization.
• Four-quadrant sector chopper enables real-time, beam-by-beam dark-current correction, eliminating drift artifacts and enhancing photometric reproducibility across extended measurement sessions.
• Large-sample compartment (210 × 290 × 200 mm) accommodates diverse accessories without optical realignment—supporting integration of integrating spheres (e.g., 60-mm PTFE-coated sphere), variable-angle specular reflectance stages, fiber-optic probes, and temperature-controlled cuvette holders.
• High-stability optical bench with thermally compensated mechanical design minimizes thermal drift; baseline stability remains within ±0.0003 A over 2 hours at 25 °C ambient.
• Photometric linearity verified to 8.0 absorbance units (A), enabling direct quantification of concentrated solutions without dilution—particularly valuable in polymer additive analysis and pigment dispersion studies.

Sample Compatibility & Compliance

The Lambda 850 supports standard 10-mm pathlength quartz and fused silica cuvettes, microvolume cells (down to 2 µL), solid-sample holders for transmission/reflection, and custom fixtures for irregular geometries. It complies with key international standards including ASTM E275 (spectrophotometer performance verification), ISO 6223 (UV/Vis instrument calibration), and USP (spectrophotometric absorption measurements in pharmaceutical quality control). When operated with UV WinLab software under configured audit-trail and electronic signature settings, the system supports 21 CFR Part 11 compliance for regulated environments—enabling secure user access control, change tracking, and raw data integrity logging required for GMP/GLP audits.

Software & Data Management

UV WinLab is supplied as standard, offering validated workflows for kinetic assays (KinLab), biomolecular quantitation (BioLab), and multi-component spectral deconvolution. Optional Advanced Spectral Software Package (ASSP) extends functionality to colorimetry (CIE 1931/1976, D65 illuminant), optical filter characterization (edge steepness, blocking range), architectural glass solar heat gain coefficient (SHGC) calculation, and mathematical operations (derivatives, smoothing, matrix algebra). All spectral data are stored in vendor-neutral .spc format with embedded metadata (wavelength calibration date, lamp usage hours, monochromator alignment status), facilitating traceable data exchange with LIMS and ELN platforms.

Applications

This refurbished Lambda 850 is routinely deployed in academic research laboratories for nanoparticle plasmon resonance mapping, in QC labs for API purity assessment per ICH Q2(R2), in materials science facilities for bandgap determination of semiconductors via Tauc plot analysis, and in environmental testing for COD and heavy metal speciation using EPA-approved colorimetric methods. Its NIR capability (up to 900 nm) supports rapid quantification of moisture content in pharmaceutical granules and polymer crystallinity index evaluation—applications where single-beam instruments lack the stability and dynamic range required for robust method transfer.

FAQ

Is this instrument factory-refurbished or third-party refurbished?
This Lambda 850 has undergone comprehensive refurbishment and functional validation by SpectraLab Scientific, including optical alignment verification, wavelength calibration traceable to NIST standards, photometric accuracy testing, and full system diagnostics. No OEM service documentation is included, but all critical subsystems (monochromators, detector housings, lamp power supplies) have been inspected and replaced where necessary.
What documentation accompanies the instrument?
A detailed refurbishment report, performance verification certificate (including measured stray light, baseline flatness, and wavelength accuracy), and original PerkinElmer operation manual (digital copy) are provided. IQ/OQ protocols are available upon request for GxP-compliant installations.
Can this system be integrated into an existing LIMS environment?
Yes—UV WinLab supports ASCII and .csv export with customizable field delimiters and timestamp formatting. The software also provides COM-based automation interfaces compatible with LabVIEW and Python (via pywin32), enabling scheduled acquisition and metadata tagging for automated batch processing.
Are replacement lamps and gratings available?
Original PerkinElmer replacement lamps (deuterium L2D2 and tungsten-halogen L2W2) and certified grating assemblies remain available through authorized distributors. SpectraLab maintains a spare parts inventory for critical wear items, including chopper motors and detector high-voltage modules.
Does the 6-month warranty cover software updates and remote diagnostics?
The warranty covers hardware defects and performance deviations from published specifications. Software updates (including security patches and minor feature enhancements) are provided free of charge during the warranty period. Remote diagnostic support via TeamViewer or AnyDesk is included for troubleshooting connectivity, acquisition errors, or accessory communication faults.