Persee TU-1810 Series Ultraviolet-Visible Spectrophotometer
| Brand | Persee |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Instrument Architecture | Single-Beam |
| Detector | Photomultiplier Tube (PMT) |
| Wavelength Range | 190–1100 nm |
| Wavelength Scanning Mode | Motorized Automatic Wavelength Drive |
| Spectral Bandwidth | 0.5 / 1.0 / 2.0 / 5.0 nm (TU-1810S models, variable slit) |
| Wavelength Accuracy | ±0.3 nm (with automatic on-boot wavelength calibration) |
| Wavelength Repeatability | ≤0.2 nm |
| Stray Light | ≤0.02% T at 220 nm (using NaI solution), ≤0.05% T at 360 nm (using NaNO₂ solution) |
Overview
The Persee TU-1810 Series Ultraviolet-Visible Spectrophotometer is a precision single-beam optical instrument engineered for quantitative and qualitative analysis across the ultraviolet and visible spectral regions (190–1100 nm). It operates on the fundamental principle of Beer–Lambert law-based absorbance measurement, where sample transmittance or absorbance is determined by comparing incident and transmitted radiant intensity through a defined optical path. The system employs a Czerny–Turner (C-T) monochromator with optimized grating dispersion and aberration correction to ensure high wavelength fidelity and minimal spectral distortion. A high-gain photomultiplier tube (PMT) serves as the detector, delivering superior signal-to-noise ratio (SNR) in low-light UV regions—critical for trace-level quantification of nucleic acids, proteins, and pharmaceutical actives. Its motor-driven automatic wavelength drive enables programmable scanning, peak search, and fixed-wavelength kinetic measurements without manual intervention.
Key Features
- Crossed Czerny–Turner monochromator design with thermally stabilized optics for reduced drift and enhanced long-term photometric stability
- Automated on-power-up wavelength calibration using internal holmium oxide or deuterium reference lines—ensuring traceable accuracy per ISO 17025 metrological requirements
- Variable spectral bandwidth selection (0.5/1.0/2.0/5.0 nm) on TU-1810S variants to balance resolution and throughput; fixed 2.0 nm bandwidth on standard TU-1810 for routine QC applications
- Stray light performance compliant with ISO 9022-11 and ASTM E275—validated at critical wavelengths (220 nm and 360 nm) using certified NaI and NaNO₂ solutions
- Integrated 7-inch capacitive touchscreen with intuitive icon-driven UI, supporting multilingual operation (English, Spanish, French, Chinese) and context-sensitive help
- Rugged aluminum alloy optical bench with vibration-damped base plate—designed for stable operation in shared laboratory environments without external optical tables
Sample Compatibility & Compliance
The TU-1810 Series accommodates standard 10 mm square quartz, glass, or plastic cuvettes (pathlengths: 1–100 mm via optional holders), as well as microvolume platforms (e.g., 1 µL capillary cells) and fiber-optic probes for in-situ monitoring. It supports compliance workflows aligned with USP , EP 2.2.25, and JP 17 Chapter 4.2.1 for spectrophotometric assay validation. Data integrity safeguards include electronic audit trails, user access levels (admin/operator/guest), and password-protected method storage—facilitating adherence to FDA 21 CFR Part 11 and EU Annex 11 when paired with Persee’s optional LIMS-integrated software module.
Software & Data Management
Persee UVWinPro v3.5 software provides full instrument control, spectral acquisition, multi-point calibration (linear, quadratic, cubic), and GLP-compliant reporting. Raw absorbance/transmittance data are exported in ASCII, CSV, or JCAMP-DX formats for third-party chemometric analysis (e.g., MATLAB, Unscrambler). The software embeds built-in validation tools—including wavelength accuracy verification, photometric linearity assessment (per ISO 6223), and baseline stability monitoring over 2-hour intervals. All method files include metadata stamps (operator ID, timestamp, instrument serial number, environmental temperature/humidity logs from optional sensor integration).
Applications
This spectrophotometer is routinely deployed in regulated and research settings for: protein concentration determination (A280), DNA/RNA purity assessment (A260/A280, A260/A230 ratios), enzyme kinetics (NADH/NADPH oxidation at 340 nm), pharmaceutical dissolution testing (USP Apparatus II with flow cell integration), heavy metal quantification via colorimetric chelation assays (e.g., Fe²⁺ with 1,10-phenanthroline), and environmental water quality monitoring (nitrate at 220 nm, phosphate via molybdenum blue at 880 nm). Its robustness and reproducibility make it suitable for ISO/IEC 17025-accredited testing laboratories performing routine QC in food safety, clinical diagnostics, and polymer additive analysis.
FAQ
Does the TU-1810 series support dual-beam functionality?
No—the TU-1810 is a single-beam system. However, its high-stability optical architecture and automated reference correction routines provide photometric repeatability comparable to entry-level double-beam instruments.
Is PMT detector replacement user-serviceable?
Detector replacement requires factory recalibration and alignment; it is not field-serviceable. Persee recommends scheduled PMT gain verification every 6 months using NIST-traceable neutral density filters.
Can the instrument be integrated into an automated liquid handling workflow?
Yes—via RS-232 and USB virtual COM port interfaces, enabling bidirectional communication with robotic platforms (e.g., Hamilton STAR, Tecan Freedom EVO) using SCPI command sets.
What validation documentation is supplied with the system?
Each unit ships with a Factory Acceptance Test (FAT) report, wavelength accuracy certificate (traceable to NIM Beijing), and IQ/OQ protocol templates compliant with GMP Annex 15 and ISO/IEC 17025 clause 6.4.1.
Is firmware upgradable in the field?
Yes—firmware updates are delivered via USB flash drive and installed through the embedded maintenance menu, preserving all user methods and calibration history.
