PuriMaster-3500C1 Preparative Liquid Chromatography System
| Brand | KEZHE SHANGHAI |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Domestic |
| Model | PuriMaster-3500C1 |
| Application Level | Laboratory-grade |
| Instrument Type | High-Pressure Preparative Liquid Chromatograph |
| Pump Pressure Rating | 300 bar |
| Flow Rate Range | 0–50 mL/min per pump |
| Flow Stability | ≤2% RSD |
| UV-Vis Detection Wavelength Range | 190–850 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | 0.2 nm |
| Detector Light Source | Dual-lamp system (Deuterium + Tungsten) |
| Fraction Collection | 6-port switching valve (standard) |
| Auto-sampler Quantitative Loop | 3 mL |
| Gradient Capability | Binary high-pressure mixing |
Overview
The PuriMaster-3500C1 Preparative Liquid Chromatography System is a laboratory-grade, high-pressure preparative HPLC platform engineered for the isolation and purification of structurally complex or closely eluting compounds—particularly those resistant to conventional single-pass separation. Developed by KEZHE SHANGHAI—a Shanghai-based OEM specializing in analytical instrumentation—the system integrates binary high-pressure gradient pumping, dual-wavelength UV-Vis detection, and intelligent fraction collection into a unified architecture optimized for reproducible, scalable purification workflows. Its core design principle follows the principles of high-resolution chromatographic separation under controlled pressure and flow dynamics, with emphasis on column protection, solvent management, and operational traceability. Unlike analytical systems, the PuriMaster-3500C1 supports both discrete batch-mode and continuous-loop operation modes, enabling iterative re-injection of partially resolved fractions without column re-equilibration—critical for isolating positional isomers, conjugated polymers (e.g., cyclized polythiophenes), fullerenes, natural pigments, and photoelectronic intermediates.
Key Features
- High-stability binary high-pressure gradient pump delivering up to 300 bar with pressure pulsation ≤0.2 MPa and flow stability ≤2% RSD across 0–50 mL/min range.
- Dual-lamp UV-Vis detector (190–850 nm) featuring automatic lamp self-diagnosis, wavelength auto-calibration (accuracy ±1 nm, repeatability 0.2 nm), and real-time baseline monitoring.
- Intelligent fraction collection with multi-modal triggering: time-based, peak-threshold-based, or absorbance-triggered collection via integrated chromatographic signal analysis.
- Comprehensive software control including column overpressure alarm & automatic pressure-limiting shutdown, solvent level sensing with configurable low-volume alerts, and one-click system purge/cleanout protocols.
- Support for both manual and automated injection modes; 3 mL fixed-loop autosampler enables large-volume sample loading without hardware modification.
- Full-cycle operational logging with audit trail capability compliant with GLP-aligned data integrity requirements, including user authentication, action timestamping, and immutable event records.
- Modular hardware configuration: standard 6-port collection valve; optional 160-position fraction collector (15 × 150 mm tube format); optional bypass switching module for column regeneration or parallel column use.
Sample Compatibility & Compliance
The PuriMaster-3500C1 accommodates a broad spectrum of preparative-scale applications, including but not limited to: purification of regioisomeric organic semiconductors, chiral and diastereomeric natural products, synthetic oligomers, and macromolecular species separable via GPC-mode cycling. It supports reversed-phase, normal-phase, and size-exclusion chromatography columns with internal diameters ranging from 10 mm to 50 mm and lengths up to 250 mm. The system meets fundamental mechanical and electrical safety standards applicable to laboratory instrumentation in CE-marked jurisdictions. While not pre-certified to FDA 21 CFR Part 11, its software architecture includes role-based access control, electronic signature support, and exportable raw data files (ASCII/CSV), facilitating validation under ISO/IEC 17025, USP , or internal GMP/GLP quality systems.
Software & Data Management
The proprietary preparative chromatography workstation provides an integrated environment for method development, execution, and post-run analysis. Key capabilities include real-time fraction indexing synchronized with chromatogram peaks, customizable PDF report generation with editable templates, spectral deconvolution and peak integration (with baseline correction algorithms), and cross-platform data portability via encrypted backup archives. All user actions—including method edits, valve actuations, and collection triggers—are logged with timestamps and operator IDs. Data export supports third-party cheminformatics platforms (e.g., ChemDraw, CSD, or OpenChrom) via standardized file formats. The software also implements automatic solvent volume tracking, predictive lamp lifetime estimation, and re-run sequence replication (“replay mode”) for method transfer consistency.
Applications
- Iterative purification of co-eluting positional isomers (e.g., ortho/meta/para-substituted aromatics or regioisomeric heterocycles) using cyclic re-injection without column reconditioning.
- Isolation of photovoltaic-active materials such as functionalized fullerenes (e.g., PCBM derivatives) and conjugated polymer fractions requiring narrow dispersity control.
- Preparative-scale separation of natural pigment mixtures (e.g., carotenoids, anthocyanins) where structural similarity demands high-resolution UV spectral discrimination.
- Gel permeation chromatography (GPC)-based fractionation of synthetic polymers followed by loop-mode reprocessing to isolate narrow-Mw cuts.
- Process development support for pharmaceutical intermediates where regulatory-compliant documentation and repeatable fraction recovery are mandatory.
FAQ
What column dimensions and chemistries are compatible with the PuriMaster-3500C1?
The system supports preparative columns with inner diameters from 10 mm to 50 mm and lengths up to 250 mm, compatible with silica-based reversed-phase (C18, C8), amino, cyano, and size-exclusion media.
Does the system support method transfer from analytical to preparative scale?
Yes—gradient delay volume compensation, dwell volume characterization, and scalable flow-rate mapping tools are embedded in the software to facilitate robust scaling from analytical UHPLC methods.
Can the software generate 21 CFR Part 11-compliant audit trails?
While not out-of-the-box certified, the system logs all critical events with user ID, timestamp, and action type; it supports export of tamper-evident audit reports suitable for internal validation against Part 11 Annex 11 or ALCOA+ principles.
Is remote monitoring or networked operation supported?
The workstation operates on Windows OS and supports LAN-based instrument control and data sharing across local networks; remote desktop access is permitted subject to institutional IT security policies.
What maintenance intervals are recommended for the UV-Vis detector lamps?
The software estimates remaining lamp life based on cumulative on-time and intensity decay trends; replacement is advised when output drops below 80% of initial intensity or after 2,000 hours of operation—whichever occurs first.
