RAININ Pipet-One Manual Single-Channel Pipette L-10PL

Overview

The RAININ Pipet-One Manual Single-Channel Pipette L-10PL is a precision-engineered liquid handling instrument designed for high-reproducibility volumetric delivery in routine and regulated laboratory environments. Based on positive displacement air-cushion aspiration and dispensing principles, the L-10PL operates within a calibrated volume range of 1–10 µL—optimized for applications requiring fine control at the microliter scale, including qPCR setup, ELISA plate preparation, nucleic acid dilution, and micro-volume cell culture reagent addition. Its mechanical architecture adheres to ISO 8655-2 (volumetric performance) and ISO 8655-6 (calibration methodology), ensuring traceability to national metrological standards. The pipette features a fully adjustable volume setting with dual-scale graduation and tactile click-stop feedback, minimizing parallax error and operator-induced variability.

Key Features

• Ergonomic finger hooks and balanced mass distribution reduce hand fatigue during extended use—validated per ISO 10993-5 cytotoxicity and ISO 7250 anthropometric guidelines.
• Low-force plunger mechanism (<1.2 N activation force at full volume) combined with optimized spring tension and low-resistance PTFE seals enables consistent aspiration across viscosity gradients (up to 100 mPa·s).
• Vibration-damping internal components minimize mechanical oscillation during tip ejection and volume adjustment, enhancing repeatability (CV ≤ 0.8% at 10 µL, per ISO 8655-2 testing protocol).
• Chemically resistant construction: anodized aluminum upper housing, stainless steel shaft, and fluoropolymer-coated internal channels ensure compatibility with common solvents (e.g., 70% ethanol, isopropanol, dilute acids/bases).
• Autoclavable at 121°C for 20 minutes (excluding tip ejector spring assembly), supporting GLP/GMP workflows requiring periodic sterilization without recalibration.
• Modular design allows field replacement of critical wear parts—including seal kits, plungers, and tip cones—extending service life beyond 100,000 actuations under standard operating conditions.

Sample Compatibility & Compliance

The L-10PL is compatible with universal conical 10 µL filter and non-filter pipette tips meeting ISO 8655-9 dimensional specifications. It maintains accuracy and precision when handling aqueous buffers, glycerol-containing solutions (≤15% v/v), and protein-rich media (e.g., BSA 1 mg/mL in PBS). Performance validation data are generated in accordance with ISO 8655-6 Annex C using gravimetric measurement (Sartorius Entris analytical balance, Class I calibration) and certified reference weights. The device conforms to EN ISO 13485:2016 for medical device manufacturing environments and supports FDA 21 CFR Part 11 compliance when integrated with RAININ’s LiteTouch™ calibration management software (optional add-on).

Software & Data Management

While the L-10PL is a manual pipette and does not incorporate embedded electronics or connectivity, its calibration history, maintenance logs, and performance verification records are fully compatible with laboratory information management systems (LIMS) via standardized CSV export from RAININ’s Pipette Calibration Manager (PCM) desktop application. PCM generates audit-trail-enabled reports compliant with GLP requirements, including operator ID, date/time stamps, environmental conditions (temperature/humidity), and pass/fail status against ISO-defined tolerance limits (±1.5% at nominal volume). Calibration certificates include unique serial-number traceability and optional UKAS accreditation linkage.

Applications

• Quantitative reverse transcription PCR (RT-qPCR) master mix preparation requiring sub-microliter precision.
• High-throughput screening (HTS) assay development where inter-operator reproducibility must remain below 1.2% CV across multi-day campaigns.
• Clinical diagnostics sample dilution workflows subject to CAP/CLIA proficiency testing requirements.
• Cell-based assay dosing in 384-well microplates, particularly for CRISPR guide RNA transfection reagents.
• Reference material preparation in metrology laboratories performing primary calibration of volumetric glassware.

FAQ

Is the L-10PL suitable for handling viscous or volatile liquids?
Yes—its low-resistance sealing system and optimized piston inertia allow reliable operation with liquids up to 100 mPa·s viscosity; however, for volatile solvents (e.g., chloroform, acetone), pre-wetting and reverse pipetting techniques are recommended.
Can the L-10PL be sterilized in an autoclave?
The main body and lower assembly (excluding the tip ejector spring and O-rings) are autoclavable at 121°C for 20 minutes; replacement seals must be installed post-sterilization to maintain ISO 8655 compliance.
What calibration documentation is provided with purchase?
Each unit ships with a factory-issued calibration certificate referencing ISO 8655-6, including gravimetric test data at 1 µL, 5 µL, and 10 µL, ambient lab conditions, and uncertainty budget per EURACHEM/CITAC Guide.
Does RAININ offer service plans for the Pipet-One series?
Yes—authorized service centers provide annual preventive maintenance (PM) packages including full disassembly, seal replacement, torque verification, and recalibration with NIST-traceable standards.
How does the Pipet-One compare to electronic pipettes in terms of long-term cost of ownership?
With no batteries, displays, or firmware, the L-10PL eliminates electronic failure modes and associated recalibration downtime; TCO over five years is typically 35–40% lower than comparable electronic models in high-utilization core facilities.