Sanotac Purify-150 Preparative Liquid Chromatography System
| Brand | Sanotac |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Domestic |
| Model | Purify-150 |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative Liquid Chromatograph |
| Flow Rate Range | 0.01–150.00 mL/min |
| Flow Accuracy | ±1% |
| Flow Precision (RSD) | ≤0.5% |
| Maximum Operating Pressure | 20 MPa |
| Wavelength Range | 190–800 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Reproducibility | 0.2 nm |
| Baseline Noise | ±0.75×10⁻⁵ AU (254 nm, TC = 1 s) |
| Data Acquisition Frequency | 10 Hz |
Overview
The Sanotac Purify-150 Preparative Liquid Chromatography System is a high-performance, modular preparative HPLC platform engineered for reproducible isolation and purification of target compounds from complex natural and synthetic matrices. Based on high-pressure liquid chromatographic separation principles—utilizing differential partitioning of analytes between a stationary phase (e.g., C18, silica, or specialized chiral media) and a mobile phase under controlled gradient elution—the system delivers robust scalability from milligram to multi-gram purification batches. Designed specifically for laboratories engaged in phytochemical research, natural product chemistry, synthetic organic methodology development, and early-stage biopharmaceutical process support, the Purify-150 integrates dual high-precision reciprocating piston pumps, a dual-wavelength UV-Vis detector with real-time spectral monitoring, and an intelligent fraction collector—all coordinated via Sanochrom™ chromatography software compliant with regulatory data integrity standards.
Key Features
- High-stability dual-pump gradient system with real-time online adjustment of flow rate and gradient profile—enabling method optimization without system shutdown.
- Microprocessor-controlled pump architecture featuring pressure feedback compensation, solvent compressibility correction, and floating plunger design to extend seal lifetime under sustained 20 MPa operation.
- Dual-wavelength simultaneous detection (190–800 nm) with wavelength programming, stop-flow scanning, and time-resolved absorbance acquisition—minimizing baseline drift and maximizing signal-to-noise ratio.
- Low-pulse flow delivery (<0.5% RSD across full range) achieved through cam-profile pulsation damping and multi-point volumetric calibration across 0.01–150.00 mL/min.
- Automated fraction collector with hybrid linear-rotary motion mechanics, sub-millisecond valve switching, independent waste channel routing, and delay-volume compensation for precise peak cutting.
- Modular hardware design supporting optional PEEK pump heads, extended column oven integration, and scalable collection racks (up to 120 × 13–15 mm tubes).
- Deuterium/tungsten lamp auto-switching mechanism and tool-free flow cell replacement—reducing instrument downtime and maintenance overhead.
Sample Compatibility & Compliance
The Purify-150 accommodates diverse sample formats including crude herbal extracts, fermentation broths, reaction mixtures, and partially purified fractions—compatible with reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatography modes. Its fluidic path employs 316L stainless steel and chemically resistant wetted materials (with PEEK options available), ensuring compatibility with aggressive solvents (e.g., DMSO, THF, TFA) and pH-stable operation (pH 2–12). The system meets essential regulatory expectations for laboratory-scale purification workflows: hardware and software architecture supports audit trails, electronic signatures, role-based access control, and data immutability per FDA 21 CFR Part 11 and CFDA GxP guidelines. All chromatographic data—including raw detector signals, gradient profiles, collection logs, and instrument status timestamps—are stored in secure, timestamped, and version-controlled project files.
Software & Data Management
Sanochrom™ workstation software provides unified control of all system modules via a Windows-native GUI (Windows 7/8/10 compatible). It supports method development tools such as gradient scouting, retention modeling, and peak tracking; enables real-time visualization of dual-wavelength chromatograms, system pressure, flow stability, and fraction positioning; and exports structured data in ASTM E1381-compliant ASCII formats for LIMS integration. Built-in validation features include IQ/OQ documentation templates, user action logging, and electronic signature workflows aligned with GLP/GMP-aligned lab practices. Raw data files are digitally signed and checksum-verified upon save; archived projects retain full metadata traceability for retrospective review or regulatory inspection.
Applications
- Isolation of reference standards from traditional Chinese medicinal herbs (e.g., ginsenosides, berberine, artemisinin derivatives) with batch-to-batch consistency verification.
- Purification of synthetic intermediates and final active pharmaceutical ingredients (APIs) where removal of regioisomers or residual catalysts is critical.
- Downstream processing of peptides and small proteins following solid-phase synthesis or enzymatic cleavage.
- Separation of enantiomeric pairs using chiral stationary phases for pharmacological activity profiling.
- Preparative-scale cleanup of natural product libraries prior to high-throughput screening assays.
- Method transfer from analytical to preparative scale using retention time normalization and linear velocity scaling algorithms embedded in Sanochrom™.
FAQ
What regulatory standards does the Purify-150 software comply with?
Sanochrom™ software conforms to FDA 21 CFR Part 11 requirements for electronic records and signatures, including audit trail generation, user authentication, and data integrity safeguards.
Can the system operate with non-aqueous mobile phases such as hexane or chloroform?
Yes—the fluidic path is constructed from 316L stainless steel and optionally PEEK components, enabling compatibility with low-polarity solvents when appropriate seals and detectors are selected.
Is method transfer support available between analytical and preparative scales?
Sanochrom™ includes retention modeling tools and linear velocity scaling utilities to facilitate systematic method translation while preserving selectivity and resolution.
How is fraction collection accuracy ensured for narrow or overlapping peaks?
The system employs real-time slope-threshold triggering, user-defined delay volume compensation, and high-speed fraction valve actuation (≤100 ms response) to minimize cross-contamination and maximize purity yield.
Does the Purify-150 support column oven integration?
Yes—external column ovens with temperature control (ambient to 80°C) can be interfaced via analog I/O or digital RS-232 communication for thermally sensitive separations.
