Shanghai Shiping SPH-103B Benchtop Incubated Orbital Shaker

Overview

The Shanghai Shiping SPH-103B Benchtop Incubated Orbital Shaker is an integrated laboratory instrument engineered for precision-controlled cultivation and agitation of biological samples under stabilized thermal conditions. It operates on the principle of orbital shaking—generating uniform circular motion with a fixed 25 mm orbit diameter—to ensure consistent suspension, aeration, and mass transfer in liquid cultures. Designed for continuous operation in regulated environments, the unit combines PID-based temperature regulation (±0.1 °C accuracy) with high-reproducibility speed control (±1 rpm), enabling reliable replication of physiological and biochemical growth conditions across microbiological, molecular, and biopharmaceutical workflows.

Key Features

• Robust mechanical architecture featuring a single-axis balanced drive system based on a parallelogram dynamic equilibrium mechanism—minimizing mechanical friction, eliminating vibration-induced deviation, and ensuring silent operation (<45 dB(A)) during extended runs.
• High-strength structural components: mainframe fabricated from high-grade ductile cast iron; shaft machined from normalized 45# carbon steel (220–250 HBW); dual-row imported bearings rated for ≥100,000 hours of continuous service life.
• GMP-compliant interior chamber constructed entirely from mirror-finish 304 stainless steel; removable stainless steel wire-mesh or spring-loaded platform (260 × 260 mm) compatible with universal clamping accessories.
• Thermal management system with 150 W heating capacity, forced-air convection circulation, and ±1 °C temperature uniformity across the 22 L working volume (internal dimensions: 330 × 305 × 215 mm).
• Intuitive LCD interface with PID microprocessor control, supporting up to eight-segment programmable ramp/soak profiles, automatic restart after power interruption, parameter encryption, and factory-reset functionality.
• Triple-layer safety architecture: independent overtemperature cutoff, overspeed alarm with audible/visual alert, real-time sensor fault diagnostics, leakage current protection, and insulated tempered glass lid with low thermal transmittance.

Sample Compatibility & Compliance

The SPH-103B accommodates standard culture vessels including Erlenmeyer flasks (up to 250 mL × 5), test tubes, microtiter plates, and enzymatic assay plates via interchangeable mounting options—bottle clamps, spring platforms, tube racks, adhesive mats, and ELISA plate holders. Its stainless-steel wetted surfaces meet material compatibility requirements for aqueous, buffered, and mildly acidic/basic media used in cell line expansion, recombinant protein expression, microbial fermentation, and immunoassay development. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, the instrument’s data logging capabilities (time-stamped temperature/speed records), parameter traceability, and hardware-level fault detection support alignment with GLP/GMP documentation practices when integrated into validated laboratory workflows.

Software & Data Management

The embedded controller provides non-volatile memory for storing up to 10 user-defined protocols, including multi-step temperature–speed sequences with dwell times. All operational parameters—including setpoints, actual readings, elapsed time, and alarm events—are logged locally with timestamp resolution of 1 second. USB export functionality enables transfer of CSV-formatted datasets for external analysis in Excel, MATLAB, or LIMS-compatible platforms. Audit trail features include password-protected parameter modification logs and automatic retention of calibration offsets for temperature and rotational speed sensors—facilitating internal quality audits and method verification per ISO 13485 or USP guidelines.

Applications

• Bacterial and yeast cultivation under controlled aeration and thermal regimes for antibiotic susceptibility testing and metabolic profiling.
• Plasmid amplification and protein expression in E. coli and mammalian suspension cultures.
• Enzyme kinetics assays requiring homogeneous mixing and stable incubation temperatures between 5 °C and 60 °C.
• Tissue dissociation and primary cell passaging where gentle yet consistent agitation prevents shear damage.
• Environmental monitoring applications such as BOD determination, water toxicity screening, and bioremediation studies.
• Pharmaceutical QC procedures including dissolution testing support, stability-indicating assay preparation, and excipient compatibility trials.

FAQ

What is the maximum flask capacity supported by the SPH-103B?

The unit supports up to five 250 mL Erlenmeyer flasks, nine 100 mL flasks, or nine 50 mL flasks simultaneously on its single 260 × 260 mm platform.
Does the shaker support programmable temperature ramps?

Yes—the PID controller allows up to eight programmable segments for simultaneous temperature and speed profiling, with adjustable ramp rates and hold durations.
Is the instrument compliant with international electrical safety standards?

It meets IEC 61010-1:2010 requirements for laboratory equipment safety and is supplied with CE-marked power input (AC 200–240 V, 50–60 Hz).
Can the SPH-103B operate unattended overnight?

Yes—its timer function supports up to 999.59 hours of continuous operation, and built-in safety interlocks (overtemp/overspeed cutoffs, auto-restart logic) ensure safe long-duration runs.
What maintenance is required for long-term reliability?

Routine cleaning of the stainless-steel chamber and platform is recommended after each use; bearing lubrication is not required due to the sealed, lifetime-rated imported bearings.