Shimadzu LC-2010C HT High-Performance Liquid Chromatograph (Refurbished)

Overview

The Shimadzu LC-2010C HT is a fully integrated, high-reliability high-performance liquid chromatograph engineered for precision, reproducibility, and method robustness in regulated and research laboratories. Built upon Shimadzu’s proven LC-2010 platform, the “C HT” variant incorporates a Peltier-cooled autosampler—enabling stable temperature control of thermolabile or volatile samples during extended unattended runs. The system operates on a unified hydraulic architecture: a low-pulsation dual-plunger pump delivers gradient elution via constant-pressure mixing, while a 5-channel vacuum membrane degasser ensures consistent mobile phase stability across all solvents. Its modular design minimizes inter-unit variability, facilitating seamless method transfer between instruments and laboratories. The LC-2010C HT was originally developed to meet stringent analytical requirements in pharmaceutical QC, environmental testing, and natural product isolation—where retention time stability, peak shape fidelity, and quantitative repeatability are non-negotiable.

Key Features

• Micro-stroke dual-plunger pump: Delivers ultra-low pulsation flow (<0.05% RMS), enabling baseline stability critical for trace-level UV detection and mass spectrometry coupling.
• Peltier-cooled autosampler (4–45 °C): Maintains sample integrity through active cooling and integrated anti-condensation dehumidification—eliminating cold-trap artifacts and carryover risks associated with ambient-temperature injection.
• High-precision full-loop injection: Achieves ≤0.3% RSD injection volume repeatability across 350 positions (1.5 mL vials) or up to four 96-well microplates—validated per ASTM E275 and USP .
• Thermostatted column oven: Semiconductor-based heating/cooling provides uniform thermal control (±0.1 °C) across the entire column bed, minimizing axial temperature gradients that compromise resolution and retention time reproducibility.
• Low-noise UV-Vis detector: Features a thermostatted flow cell and built-in low-pressure mercury lamp for automatic wavelength calibration—ensuring long-term photometric accuracy and compliance with ISO 17025 traceability requirements.
• Integrated 5-channel degasser: Reduces dissolved gas content below 5 ppm O₂, preventing bubble formation in high-sensitivity detection and improving gradient reproducibility over multi-day sequences.

Sample Compatibility & Compliance

The LC-2010C HT supports a broad range of sample matrices—including aqueous extracts, organic extracts, biological fluids, and synthetic intermediates—across conventional (4.6 mm ID), narrow-bore (2.1 mm ID), and semi-micro (1.0 mm ID) column formats. Its autosampler accommodates standard 1.5 mL screw-thread vials, crimp-top vials, and ANSI/SLAS-compliant microtiter plates. All fluidic pathways use chemically inert stainless steel and PEEK components rated for pH 1.5–10.5 and pressures up to 40 MPa (5800 psi). The system architecture complies with IEC 61010-1 safety standards and is designed to integrate into laboratories operating under GLP, GMP, and ISO/IEC 17025 quality management systems. When paired with Shimadzu LabSolutions software (v5.9+), audit trails, electronic signatures, and 21 CFR Part 11–compliant data handling are achievable.

Software & Data Management

The LC-2010C HT communicates natively with Shimadzu LabSolutions LCMS software via Ethernet or RS-232. LabSolutions provides intuitive method setup wizards, real-time chromatogram visualization, peak integration with customizable baselines, and automated calibration curve generation. Raw data files (.lcd) are stored in vendor-neutral format with embedded metadata—including instrument configuration, sequence logs, and detector parameters—supporting long-term archival and reprocessing. The system supports LIMS integration via OpenLAB CDS or third-party middleware compliant with ASTM E1578 and HL7 standards. All firmware versions installed during refurbishment are validated against Shimadzu’s official release notes, and full calibration certificates (flow rate, dwell volume, injection volume, wavelength accuracy) are provided with each unit.

Applications

This refurbished LC-2010C HT is routinely deployed in applications demanding high inter-day reproducibility and regulatory readiness: stability-indicating assays for pharmaceuticals (ICH Q2(R2)), residual solvent analysis per USP , pesticide residue screening in food (AOAC 2007.01), PAH quantification in environmental water (EPA Method 8310), and organic acid profiling in fermentation broths. Its cooled autosampler makes it especially suitable for analyzing labile compounds such as aldehydes, epoxides, and oligonucleotides—where degradation at ambient temperature would otherwise compromise quantitation. Academic labs utilize its modularity for method development studies involving column switching, heart-cutting 2D-LC, and post-column derivatization setups.

FAQ

Is this unit fully refurbished and tested prior to shipment?
Yes. Each LC-2010C HT undergoes comprehensive functional verification—including pump flow accuracy, gradient composition linearity, autosampler precision, detector noise and linearity, and column oven thermal uniformity—per Shimadzu’s OEM test protocols.
What documentation accompanies the instrument?
You receive a full refurbishment report, calibration certificates for all critical subsystems, a 6-month limited hardware warranty, and a traceable chain-of-custody record documenting service history and component replacements.
Can this system be upgraded to support modern data integrity requirements?
Yes. With LabSolutions LCMS v5.9 or later and optional 21 CFR Part 11 modules, the system meets FDA and EMA expectations for electronic records and signatures when configured with appropriate IT infrastructure and SOPs.
Are spare parts and consumables readily available?
All major consumables—including pump seals, check valves, autosampler rotors, UV lamps, and degasser membranes—are stocked by global Shimadzu distributors and remain in production. Critical wear parts are included in the refurbishment package.
Does the system include original accessories and cables?
Yes. The shipment includes all factory-original modules, power cables, communication cables, two 1.5 mL vial racks, user manuals (English), and a complete set of installation tools.