WX-ZW108 Vientiane Environment Single-Channel 8-Well Real-Time Fluorescent PCR Analyzer for African Swine Fever Virus Detection

Overview

The WX-ZW108 is a compact, single-channel real-time fluorescent quantitative PCR (qPCR) analyzer engineered specifically for field-deployable and laboratory-based nucleic acid detection of African Swine Fever virus (ASFV). It operates on the principle of sequence-specific amplification coupled with real-time fluorescence monitoring—utilizing intercalating dyes (e.g., SYBR Green I) or hydrolysis probes (e.g., FAM-labeled) to quantify target ASFV genomic DNA during thermal cycling. Designed in compliance with China’s Ministry of Agriculture and Rural Affairs (MARA) regulatory framework for veterinary molecular diagnostics, the instrument supports only MARA-approved ASFV detection kits—including certified fluorescent PCR reagent kits for ASFV, avian influenza, classical swine fever, porcine reproductive and respiratory syndrome (PRRS), and pseudorabies. Its portability, robust thermal architecture, and embedded analytical software enable reliable deployment in mobile biosafety units, slaughterhouse QC labs, feed mills, and regional animal health laboratories where rapid, traceable, and auditable pathogen detection is operationally critical.

Key Features

• Industrial-grade Peltier thermocycling module with high-precision PT1000 temperature sensing—achieving ±0.1 °C thermal accuracy and ≤±0.25 °C well-to-well uniformity across all 8 positions.
• Optimized optical path design featuring a long-life, high-intensity blue LED excitation source (460–480 nm) and a Japan-sourced photomultiplier tube (PMT) detector (505–525 nm), eliminating need for optical calibration or lamp replacement over instrument lifetime.
• Embedded Android operating system with intuitive touchscreen interface; supports bilingual (English/Chinese) navigation, pre-loaded assay templates, and user-defined protocol storage for rapid assay initiation.
• Real-time data acquisition and analysis including automatic baseline determination, Ct threshold assignment, standard curve fitting, and melt curve (HRM) capability—all executed onboard without external PC dependency.
• Comprehensive data governance: audit-trail-enabled session logging, experiment metadata tagging, timestamped result export (CSV/PDF/Excel), and Wi-Fi/USB-based transfer to LIMS or cloud repositories—aligned with GLP-aligned documentation practices.
• Acoustically optimized mechanical housing (<60 dB operational noise) and sealed aluminum reaction block resistant to condensation ingress, ensuring long-term stability in humid or variable ambient environments.

Sample Compatibility & Compliance

The WX-ZW108 accepts standard 0.2 mL single tubes and 8-well strips, accommodating sample volumes from 15–100 µL. Its open reagent architecture permits use with any commercially available qPCR master mix and probe/dye chemistry validated for ASFV detection—including MARA-registered kits compliant with GB/T 38927-2020 (Nucleic Acid Detection Methods for African Swine Fever Virus). The instrument meets electromagnetic compatibility (EMC) requirements per GB/T 18268.1–2010 and safety standards under GB 4793.1–2007. While not FDA 510(k)-cleared or CE-IVD marked, its design philosophy aligns with ISO 13485 quality system principles and supports traceable implementation under national veterinary diagnostic guidelines (NY/T 3403–2018).

Software & Data Management

The integrated analysis software provides full-cycle assay management—from experimental setup (including TouchDown and gradient ramping) to post-run quantification. Key capabilities include concurrent multi-experiment review, group-wise comparative analysis of replicate runs, auto-generated Ct tables with confidence intervals, and customizable report templates adhering to MARA submission formats. All raw fluorescence curves, amplification plots, and metadata are stored locally with SHA-256 hashed integrity verification. Export options include encrypted USB transfers and authenticated Wi-Fi push to designated network shares—facilitating integration into centralized veterinary surveillance platforms. Software update packages are delivered via secure OTA channel with version-controlled changelogs and rollback support.

Applications

Primarily deployed for frontline ASFV surveillance, the WX-ZW108 serves as a validated endpoint in national swine health monitoring programs. Its throughput and sensitivity make it suitable for routine screening of blood, tissue homogenates, environmental swabs, and feed samples across commercial farms, border inspection posts, and provincial animal disease prevention centers. Beyond ASFV, the platform supports differential diagnosis of co-circulating pathogens including AIV (H5/H7 subtypes), CSFV, PRRSV, and PRV—enabling multiplex-ready workflow expansion via future firmware updates. The device also functions as a training tool for veterinary technicians under MARA-certified capacity-building initiatives, emphasizing standardized nucleic acid extraction, contamination control, and result interpretation per OIE Terrestrial Manual Chapter 2.8.7.

FAQ

Is the WX-ZW108 approved for official ASFV testing under Chinese regulatory frameworks?
Yes—it is intended for use exclusively with MARA-registered ASFV qPCR kits and complies with technical specifications outlined in Notice No. 295 of 2020 issued by the Ministry of Agriculture and Rural Affairs.
Can the instrument be used with non-MARA-approved reagents?
While hardware supports open reagent use, regulatory validation and reporting validity require conformance with MARA-approved kits and associated SOPs.
Does the system support remote diagnostics or service access?
Yes—embedded diagnostics log fault codes, thermal performance drift trends, and optical signal decay metrics; these can be securely transmitted to authorized service engineers for predictive maintenance scheduling.
What is the recommended calibration interval?
No routine optical recalibration is required; thermal calibration verification is advised every 24 months using NIST-traceable reference standards.
Is the software compliant with 21 CFR Part 11 requirements?
The current version supports electronic signatures, audit trails, and role-based access control—but formal Part 11 validation must be performed by end-users within their own IT governance environment.