WX-ZW216 Dual-Channel 16-Well Real-Time Fluorescent Quantitative PCR System for African Swine Fever Virus Detection

Overview

The WX-ZW216 is a compact, dual-channel real-time fluorescent quantitative PCR system engineered specifically for field-deployable and laboratory-based nucleic acid detection of African Swine Fever Virus (ASFV) and other priority swine and avian pathogens. It operates on the principle of sequence-specific amplification coupled with real-time fluorescence monitoring—enabling absolute or relative quantification of target DNA sequences during thermal cycling. Designed in compliance with regulatory requirements for veterinary molecular diagnostics—including adherence to China’s Ministry of Agriculture and Rural Affairs (MARA) approval framework for ASF diagnostic reagents—the instrument supports validated assays for ASFV, Classical Swine Fever Virus (CSFV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Pseudorabies Virus (PRV), Avian Influenza Virus (AIV), and others. Its portability, robust thermal architecture, and optical stability make it suitable for deployment across diverse operational environments: from centralized reference laboratories and provincial animal disease control centers to mobile veterinary units, slaughterhouses, feed mills, and border inspection posts.

Key Features

• Industrial-grade Peltier thermal module with high-precision PT1000 temperature sensing, delivering ±0.1 °C accuracy and ≤±0.25 °C well-to-well uniformity across the full 4–99 °C operating range.
• Dual independent optical paths: two long-life, non-thermal LED excitation sources (460–480 nm and 525–545 nm) paired with matched narrow-band emission filters and high-sensitivity photodiode detectors—eliminating cross-talk and enabling simultaneous FAM/VIC detection without optical recalibration.
• Embedded Android-based operating system with intuitive touchscreen UI; supports preloaded assay templates, user-defined protocol storage, and one-touch run initiation.
• Optional QR-code scanning integration allows automated program selection via sample plate or assay card barcode—reducing operator error and standardizing workflow execution.
• High-speed thermal ramping (up to 6 °C/s heating, 4 °C/s cooling) reduces total assay time while maintaining stringent amplification efficiency (99–101%) and reproducibility (inter-well CV < 2%).
• Open consumables platform compatible with standard 0.2 mL single tubes and 8-tube strips from any global supplier—no proprietary cartridge lock-in.

Sample Compatibility & Compliance

The WX-ZW216 accepts biological samples processed through ISO/IEC 17025-aligned nucleic acid extraction protocols—including whole blood, serum, tissue homogenates, oral/pharyngeal swabs, and environmental swab eluates. When used with MARA-approved ASFV qPCR kits (e.g., fluorescent probe-based assays targeting the p72 or CD2v genes), the system meets national validation criteria for diagnostic sensitivity, specificity, and repeatability. Its design aligns with Good Laboratory Practice (GLP) documentation standards, supporting audit-ready data integrity through timestamped run logs, electronic signatures (where enabled), and traceable parameter metadata. While not certified under FDA 21 CFR Part 11 by default, the firmware architecture supports future configuration for regulated environments requiring electronic record controls.

Software & Data Management

The onboard analysis software provides comprehensive qPCR data processing: automatic baseline determination, adaptive threshold setting, melt curve analysis (HRM), and multi-parameter amplification curve fitting. All results—including Ct values, efficiency-corrected copy numbers, and confidence intervals—are exportable in PDF, XLS, CSV, and DOCX formats. Data transfer options include direct USB mass storage dump, Wi-Fi upload to local network servers, or cloud-synced repositories (via optional configuration). The system maintains full experimental provenance: each exported report embeds instrument ID, firmware version, thermal profile, optical calibration status, and user-defined sample annotations—ensuring full traceability per ISO/IEC 17025 Clause 7.8.

Applications

• Routine surveillance testing in commercial pig farms and breeding facilities
• Rapid confirmatory diagnosis at regional animal health laboratories
• Border biosecurity screening at ports of entry and transit checkpoints
• Feed safety monitoring and environmental sampling in processing plants
• Field epidemiological investigations during outbreak response
• Validation studies for novel ASFV diagnostic reagents under GLP conditions

FAQ

Does the WX-ZW216 support multiplex detection?
No—it is configured for dual-channel (not multiplex) detection. Each channel operates independently and is optimized for FAM- or VIC-labeled probes; simultaneous detection of two targets per reaction is possible only if spectral separation and assay validation are confirmed externally.
Is external computer connectivity required for operation?
No—the instrument runs fully autonomously using its embedded Android OS. A PC connection via USB is optional and used only for advanced data archiving, remote firmware updates, or batch report generation.
What is the recommended calibration interval?
Due to the inherent stability of the TEC module and solid-state optical components, routine calibration is not required. Annual verification against NIST-traceable temperature and fluorescence standards is advised for GLP-compliant installations.
Can the system be powered by DC or battery sources in field use?
It operates exclusively on 220 V AC input. For mobile deployment, an external pure-sine-wave inverter (≥500 VA) with stable output regulation is necessary to ensure thermal and optical performance consistency.
Are MARA-approved reagent kits included with the instrument?
No—reagents are supplied separately. The WX-ZW216 is validated for use with multiple MARA-registered ASFV qPCR kits; users must procure those directly from licensed manufacturers in accordance with national regulatory guidelines.