Yoke i5 Visible Spectrophotometer
| Brand | Yoke |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic (China) |
| Model | i5 |
| Quotation | Upon Request |
| Optical Design | Single-Beam |
| Detector | Photodiode Array (PDA) |
| Wavelength Range | 360–900 nm (Visible Region) |
| Wavelength Scanning | Automatic |
| Spectral Bandwidth | 4 nm |
| Wavelength Accuracy | ±2 nm |
| Wavelength Repeatability | ≤1 nm |
| Stray Light | ≤0.5%T at 420 nm (NaNO₂ reference) |
Overview
The Yoke i5 Visible Spectrophotometer is a precision-engineered single-beam optical instrument designed for routine quantitative and qualitative analysis in visible-light spectral regions (360–900 nm). It operates on the fundamental principle of Beer–Lambert law-based absorbance measurement, where sample transmittance (T%) and absorbance (A) are determined by comparing light intensity before and after passing through a cuvette-contained analyte. Engineered for robustness and reproducibility in QC/QA laboratories, teaching environments, and industrial process support labs, the i5 integrates a Czerny–Turner monochromator with a holographic blazed grating to ensure consistent spectral resolution and photometric linearity. Its single-beam architecture—optimized for cost-effective deployment without compromising core metrological integrity—is complemented by automated wavelength positioning, real-time photometric calibration, and dual-source illumination management (tungsten-halogen and deuterium lamp optional via firmware upgrade path), enabling reliable operation across diverse sample matrices.
Key Features
- 7-inch high-resolution capacitive touchscreen (1024 × 768 pixels) supporting intuitive gesture-based navigation and real-time visualization of spectra, kinetic traces, and quantitative results.
- Integrated Wi-Fi, Bluetooth 4.2, and USB 2.0 interfaces for seamless data export, remote firmware updates, and peripheral connectivity—including direct thermal printer output and RS-232 serial communication with legacy LIMS or PC-based acquisition software.
- Advanced optical drive mechanism delivering enhanced wavelength positioning accuracy (±2 nm) and repeatability (≤1 nm), minimizing mechanical hysteresis and operational noise during scanning routines.
- Automated wavelength calibration and T/A mode switching with zero-offset correction; built-in lamp auto-switching logic ensures optimal source selection per wavelength segment.
- Cloud-enabled data synchronization via YOKE@Cloud platform—accessible through QR-code-initiated mobile app registration—supporting secure, timestamped storage and cross-device retrieval compliant with internal audit requirements.
- Preloaded application modules: Fixed-wavelength quantitation, multi-point standard curve generation, kinetic assay monitoring (up to 999 time points), nucleic acid/protein A260/A280 ratio calculation, and derivative spectral analysis (1st/2nd order).
Sample Compatibility & Compliance
The i5 accommodates standard 10-mm rectangular quartz or glass cuvettes (pathlength 10 mm), as well as micro-volume adapters (down to 50 µL). It supports liquid-phase samples compatible with common UV-Vis solvents (e.g., water, ethanol, phosphate buffer) and is routinely deployed in accordance with ISO 6425:2018 (spectrophotometer performance verification) and ASTM E275-22 (standard practices for UV-Vis-NIR spectrophotometry). While classified as a visible-range instrument, its extended range (320–1100 nm, per specification sheet) permits limited near-UV and NIR applications—subject to user validation. The system meets IEC 61010-1 safety standards and incorporates GLP-ready features including operator ID logging, method version control, and audit-trail-capable data export (CSV/XLSX formats).
Software & Data Management
Instrument control and data processing are managed through YokeSoft v3.x—a Windows-compatible desktop application supporting method creation, spectral overlay, baseline correction, peak integration, and report generation (PDF/Excel). All raw and processed datasets retain embedded metadata: date/time stamp, operator ID, instrument serial number, and calibration history. Cloud-synced measurements are encrypted in transit (TLS 1.2+) and at rest (AES-256), with role-based access configurable per organizational policy. Data exports comply with FDA 21 CFR Part 11 requirements when used in conjunction with validated electronic signature workflows and external identity management systems.
Applications
- Routine concentration determination of dyes, pigments, and colored compounds in textile, food, and beverage quality control.
- Enzyme kinetics monitoring (e.g., NADH/NADPH oxidation at 340 nm) under controlled temperature conditions using external Peltier accessories.
- Water quality analysis: nitrate (at 220 nm, with UV extension), phosphate (via molybdenum blue method at 880 nm), and iron (1,10-phenanthroline complex at 510 nm).
- Educational use in undergraduate chemistry and biology labs for teaching spectroscopic principles, calibration curve construction, and error propagation analysis.
- Pharmaceutical excipient identification and purity screening per USP guidelines for colorimetric assays.
FAQ
Is the i5 capable of UV-range measurements below 360 nm?
The standard configuration covers 360–900 nm; however, optional firmware and hardware upgrades—including a deuterium lamp module and quartz optics—extend functionality to 190–1100 nm. Validation per ICH Q2(R2) is required for regulated UV applications.
Does the instrument support 21 CFR Part 11 compliance out-of-the-box?
Electronic signatures, audit trails, and user access controls require configuration within YokeSoft v3.x and integration with enterprise authentication services (e.g., LDAP/Active Directory). Full compliance depends on site-specific validation protocols.
Can kinetic data be exported in real time during acquisition?
Yes—via USB or Wi-Fi streaming to a connected PC running YokeSoft, with configurable sampling intervals from 0.1 s to 60 s and automatic CSV buffering during long-duration assays.
What is the maximum scan speed across the full wavelength range?
At 4-nm bandwidth and 1-nm step resolution, full-range scanning (360–900 nm) completes in approximately 12 seconds, optimized for reaction monitoring without spectral distortion.
Are third-party cuvettes compatible with the i5 sample compartment?
All standard 10-mm-pathlength square or rectangular cuvettes meeting ISO 10934-1 dimensional tolerances are supported; proprietary alignment features ensure repeatable positioning across brands.
