Yoke UV752 Ultraviolet-Visible Spectrophotometer
| Brand | Yoke |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Direct Manufacturer |
| Instrument Type | Domestic |
| Model | UV752 |
| Price Range | USD 700–1,400 (FOB) |
| Optical Design | Single-Beam |
| Detector | Photodiode Array |
| Wavelength Range | 195–1020 nm |
| Wavelength Selection | Manual |
| Spectral Bandwidth | 5 nm |
| Wavelength Accuracy | ±2 nm |
| Stray Light | ≤0.3% T |
Overview
The Yoke UV752 Ultraviolet-Visible Spectrophotometer is a precision single-beam optical instrument engineered for routine quantitative and qualitative analysis in academic teaching laboratories, quality control environments, and small-scale industrial R&D settings. It operates on the fundamental principle of Beer–Lambert law-based absorbance measurement, utilizing a Czerny-Turner monochromator equipped with a 1200 lines/mm holographic grating to resolve spectral features across the ultraviolet, visible, and near-infrared regions (195–1020 nm). Its single-beam architecture—paired with high-stability tungsten and deuterium lamps (imported), automatic lamp switching, and an integrated shutter mechanism—ensures consistent photon flux delivery while extending photodetector service life. Designed for operational simplicity and long-term reliability, the UV752 delivers traceable photometric performance without requiring complex alignment or real-time reference beam compensation.
Key Features
- Optimized optical path featuring an achromatic Czerny-Turner monochromator with 1200 L/mm grating for enhanced resolution and throughput across the full 195–1020 nm range
- High-stability dual-lamp system: imported deuterium lamp (UV) and tungsten-halogen lamp (VIS-NIR), with fully automated lamp selection based on wavelength position
- Photodiode array detector providing rapid signal acquisition and improved signal-to-noise ratio versus traditional PMT-based systems
- Manual wavelength drive with fine-tuning capability; calibrated digital readout ensures repeatable positioning within ±1 nm wavelength repeatability
- Integrated automatic shutter minimizes detector exposure during idle periods, reducing thermal drift and enhancing photodiode longevity
- Spacious sample compartment accommodating cuvettes from 5 mm to 100 mm pathlength, compatible with standard 10 mm square quartz, glass, or plastic cells
- Robust mechanical construction with 12 kg net weight, contributing to vibration resistance and baseline stability of ±0.004 A/h at 500 nm
Sample Compatibility & Compliance
The UV752 supports liquid-phase analysis using conventional transmission cuvettes and is routinely employed for concentration determination, kinetic assays, and spectral scanning of organic/inorganic compounds, biomolecules, and dyes. It complies with core photometric metrology practices aligned with ISO 6223:2020 (spectrophotometers — performance verification) and ASTM E275–22 (standard practices for describing and measuring performance of UV-Vis spectrophotometers). While not certified for GMP-regulated production environments, its documented photometric accuracy (±0.5% T), stray light performance (≤0.3% T at 220 nm), and baseline linearity meet requirements for GLP-compliant educational labs and internal QC workflows. All optical components—including grating, lamps, and detector—are sourced from internationally qualified suppliers to ensure batch-to-batch consistency.
Software & Data Management
The UV752 operates as a standalone instrument with embedded firmware controlling all core functions. Data output is facilitated via a parallel port interface supporting optional thermal printers (e.g., Epson-compatible models) for hard-copy reporting of absorbance spectra, calibration curves, or fixed-wavelength measurements. Though no PC-based software is bundled, ASCII-formatted data export enables integration into third-party analysis platforms (e.g., Excel, Origin, Python pandas) for post-acquisition processing. The instrument maintains audit-ready operational logs including lamp usage hours, shutter actuation count, and calibration timestamp—supporting basic traceability under institutional GLP frameworks. Firmware updates are available through authorized Yoke technical support channels upon request.
Applications
- Determination of analyte concentration in pharmaceutical excipients and active ingredients via standard calibration curves
- Verification of nucleic acid purity (A260/A280 ratios) and protein quantification using Bradford or BCA assays
- Monitoring chemical reaction kinetics through time-resolved absorbance at selected wavelengths
- Characterization of dye degradation pathways under UV irradiation or thermal stress
- Educational demonstration of electronic transitions in conjugated systems (e.g., π→π*, n→π*)
- Water quality assessment including nitrate, phosphate, and COD-related chromophore detection
FAQ
Is the UV752 compliant with FDA 21 CFR Part 11?
No—the UV752 does not include electronic signature capability, audit trail generation, or user access controls required for Part 11 compliance. It is intended for non-GxP environments.
Can I connect the UV752 to a computer for data capture?
The instrument lacks USB or RS-232 ports; data transfer is limited to parallel-printer output. External analog-to-digital conversion is possible but not supported by factory firmware.
What is the recommended maintenance schedule?
Lamp replacement is advised every 1,000–1,500 operating hours; optical alignment verification should occur annually or after physical relocation.
Does the UV752 support kinetic or multi-wavelength scanning modes?
No—it performs single-wavelength or manual step-scan measurements only. Continuous spectral scanning is not implemented.
Are quartz cuvettes required for UV measurements?
Yes—standard glass or plastic cuvettes absorb strongly below ~340 nm; fused silica quartz cells are mandatory for measurements between 195–340 nm.
