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Youyunpu YP-EU Series UV-Vis Spectrophotometer

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Brand Youyunpu
Origin Shandong, China
Manufacturer Type Direct Manufacturer
Product Origin Domestic (China)
Model YP-EU Series
Spectral Range 190–1020 nm
Spectral Bandwidth 4 nm
Wavelength Accuracy ±1.0 nm
Wavelength Repeatability ≤0.5 nm
Photometric Accuracy ±0.002 A (0–0.5 A), ±0.004 A (0.5–1.0 A), ±0.3% T (0–100% T)
Baseline Drift ≤0.002 A/h (at 500 nm, 0 A)
Baseline Flatness ±0.003 A

Overview

The Youyunpu YP-EU Series UV-Vis Spectrophotometer is a precision optical instrument engineered for quantitative and qualitative analysis across the ultraviolet, visible, and near-infrared spectral regions (190–1020 nm). Based on the principle of Beer–Lambert law absorption spectroscopy, it employs a dual-beam optical architecture with real-time reference beam compensation to minimize drift and enhance measurement stability over extended operation periods. This design ensures high photometric reproducibility and robust performance in routine QC laboratories, academic research settings, and regulated environments where trace-level absorbance detection and spectral fidelity are critical. The instrument integrates a long-life, flange-mounted deuterium lamp for UV emission and a tungsten-halogen lamp for visible/NIR output—both pre-aligned to eliminate post-replacement optical recalibration.

Key Features

  • Fixed 4 nm spectral bandwidth optimized for pharmacopeial compliance (e.g., USP & EP monographs requiring ≤4 nm resolution for assay validation)
  • High-stability optical bench with imported precision optical components and temperature-compensated photodetector assembly
  • Push-pull sample compartment lid enabling single-hand operation while holding cuvettes or accessories
  • Spacious sample chamber accommodating standard 5 mm to 100 mm pathlength cuvettes—including microvolume, flow-cell, and solid-sample holders
  • Large dot-matrix LCD display with intuitive icon-driven interface; supports real-time spectral visualization and parameter adjustment
  • Integrated data retention: all spectra and measurement logs persist through power interruption and support timestamped export via USB or RS-232
  • Factory-calibrated wavelength and photometric scales traceable to NIST-certified standards

Sample Compatibility & Compliance

The YP-EU Series accommodates liquid, suspension, and thin-film samples using standardized quartz, glass, or plastic cuvettes. Its 190–1020 nm spectral coverage meets requirements for nucleic acid (A260/A280) and protein (A280) quantification per ISO 8655-6 and ASTM E275. The ±0.002 A photometric accuracy in the low-absorbance range satisfies ICH Q2(R2) linearity and accuracy criteria for analytical method validation. While not inherently 21 CFR Part 11 compliant out-of-the-box, audit trail functionality, electronic signature support, and user access control can be implemented via the optional PC software package—enabling alignment with GLP/GMP documentation practices in pharmaceutical and clinical testing labs.

Software & Data Management

The spectrophotometer ships with Windows-compatible scanning software supporting full instrument control, spectral acquisition, kinetic time-course analysis, multi-wavelength quantitation, and custom method development. Raw data files (.spc, .csv) include metadata such as date/time stamp, operator ID, lamp status, and environmental conditions (if external sensor integrated). Software features include baseline correction algorithms, peak integration, derivative spectroscopy, and report generation templates compliant with ISO/IEC 17025 documentation structure. Data export supports LIMS integration via delimited text or XML schema, facilitating automated QA/QC workflows.

Applications

  • Pharmaceutical quality control: assay of active pharmaceutical ingredients (APIs), dissolution testing, excipient compatibility studies
  • Biochemical analysis: DNA/RNA purity assessment, Bradford/Lowry/BCA protein assays, enzyme kinetics (e.g., NADH oxidation at 340 nm)
  • Environmental monitoring: nitrate/nitrite, phosphate, heavy metal (e.g., Cr(VI) at 540 nm) quantification in water per EPA Method 353.2
  • Materials science: bandgap estimation of semiconductors, dye concentration profiling, thin-film thickness modeling
  • Food & beverage: colorimetric determination of antioxidants (e.g., polyphenols), preservative content (e.g., sulfites), and adulterant screening

FAQ

What is the typical warm-up time required before achieving photometric stability?
The instrument achieves thermal and lamp stabilization within 20 minutes under ambient laboratory conditions (20–25°C, <60% RH). Baseline drift remains within specification (≤0.002 A/h) after this period.
Can the YP-EU Series perform stray light measurements according to ISO 9022-3?
Yes—using the supplied sodium nitrite solution (10 g/L, 220 nm cutoff), users can verify stray light levels below 0.1%T at 220 nm, satisfying ISO 9022-3 Class 3 requirements.
Is firmware upgrade supported in the field?
Yes—firmware updates are delivered via USB drive and installed through the instrument’s maintenance menu without requiring service engineer intervention.
Does the system support external triggering for synchronized kinetic assays?
Yes—the rear-panel TTL input enables hardware synchronization with external devices such as reaction injectors or temperature controllers.
What is the warranty coverage and service support structure?
Youyunpu provides a 24-month limited warranty covering parts and labor. Technical support includes remote diagnostics, application consulting, and certified calibration services through authorized regional service centers.

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