ZHONGKEHUIFEN Series Autosamplers for Chromatography Systems

Overview

The ZHONGKEHUIFEN Series Autosamplers are precision-engineered liquid handling modules designed for seamless integration with gas chromatography (GC), liquid chromatography (LC), and high-performance liquid chromatography (HPLC) systems. These autosamplers operate on a programmable syringe-based sampling principle, enabling repeatable, low-volume injections with minimal carryover and high positional accuracy. Built to support routine analytical workflows in QC laboratories, environmental testing facilities, and pharmaceutical R&D environments, the series emphasizes reliability under continuous operation, compatibility with standard 2 mL vials and 96-well microplates, and compliance-ready operational logging. Unlike manual injection methods, these instruments reduce human variability, improve throughput consistency, and support method traceability requirements common in regulated industries.

Key Features

• Programmable injection sequence control via intuitive front-panel interface or remote command protocol (RS-232/USB)
• Adjustable injection volume range from 0.1 µL to 100 µL, with volumetric repeatability ≤ 0.5% RSD (n = 10, 10 µL standard)
• Thermally stabilized sample tray accommodating up to 120 × 2 mL vials or dual-position 96-well plate support
• Low-dead-volume fluid path constructed from chemically resistant PEEK and stainless steel components
• Auto-purge and needle wash cycles configurable per sample, supporting aqueous and organic solvent compatibility
• Integrated status monitoring with real-time error logging and hardware fault detection (e.g., syringe stall, vial misalignment)

Sample Compatibility & Compliance

The ZHONGKEHUIFEN Autosampler Series is validated for use with common chromatographic solvents including acetonitrile, methanol, water, and chloroform, and demonstrates stable performance across pH 2–12 sample matrices. It supports both volatile and non-volatile analytes without significant adsorption or degradation in the sampling loop. From a regulatory perspective, the system supports audit-trail-enabled operation when interfaced with compliant chromatography data systems (CDS), and its firmware architecture allows configuration of user access levels, electronic signatures, and instrument usage logs—features aligned with FDA 21 CFR Part 11 and ISO/IEC 17025 documentation expectations. While not certified as GMP-compliant out-of-the-box, the hardware design and software extensibility facilitate qualification protocols (IQ/OQ/PQ) required for GLP and pharmaceutical QC applications.

Software & Data Management

Autosampler operation is managed either through standalone control software (Windows-based, .NET framework) or native integration drivers for major CDS platforms including Thermo Chromeleon, Agilent OpenLab CDS, and Waters Empower. All injection parameters—including dwell time, rinse cycles, injection speed, and delay times—are stored in ASCII-readable method files, enabling version control and method transfer between instruments. Raw event logs (e.g., “vial #42 injected at 14:23:18”, “needle wash completed”) are timestamped and exportable in CSV format. Optional firmware upgrades provide enhanced scheduling logic (e.g., conditional injection based on prior peak area thresholds) and remote diagnostics via secure SSH tunneling.

Applications

• Routine quality control of raw materials and finished products in pharmaceutical manufacturing (e.g., residual solvent analysis per ICH Q3C guidelines)
• Environmental monitoring of VOCs and SVOCs in soil, water, and air samples per EPA Methods 502.2, 8260D, and 8270E
• Food safety testing for pesticide residues, mycotoxins, and preservatives using LC-MS/MS or GC-ECD workflows
• Academic and industrial research requiring unattended overnight sequencing of >100 samples with consistent injection precision
• Method development labs evaluating column robustness and detector linearity across variable injection volumes and matrix types

FAQ

Is this autosampler compatible with third-party GC and HPLC systems?
Yes—it supports standard TTL trigger signals and RS-232 command sets widely adopted across OEM platforms; custom driver development is available upon request.
What maintenance intervals are recommended for long-term reliability?
Syringe calibration verification every 2000 injections; needle and septum replacement every 5000 injections or quarterly—whichever occurs first.
Can the autosampler be operated in a nitrogen-purged environment?
Yes—the electronics housing meets IP20 rating and has no internal oxygen-sensitive components; optional inert-gas purge ports are available for glovebox integration.
Does the system support partial-loop injection mode?
Yes—both full-loop and partial-loop injection modes are supported, with programmable fill volume and injection volume independence.
Are method files backward-compatible across firmware versions?
Method files created in v2.x and later are fully backward-compatible; v1.x files require conversion via the included migration utility.