Zhongwei ZWY-211E Large-Capacity Full-Temperature Incubator Shaker

Overview

The Zhongwei ZWY-211E Large-Capacity Full-Temperature Incubator Shaker is an engineered platform for precise, scalable, and reproducible cultivation of microbial, cellular, and enzymatic cultures under tightly controlled thermal and mechanical conditions. Designed for process development, pilot-scale fermentation, and high-throughput bioprocessing, it integrates a forced-convection temperature-controlled chamber with a robust orbital shaking mechanism—operating on the principle of uniform circular motion (Φ26 mm orbit) to ensure homogeneous suspension, oxygen transfer, and nutrient distribution across large-volume vessels. Its 256 L internal volume and reinforced load-bearing shelf (920 × 500 mm) accommodate up to six 5 L flasks or fifteen 1 L vessels simultaneously—making it suitable for applications ranging from recombinant protein expression to antibiotic production and cell line expansion. The unit maintains stable setpoints between 4 °C and 60 °C, with resolution of 0.1 °C and uniformity ≤±1 °C at 37 °C—meeting foundational requirements for GLP-compliant microbiological growth studies and ISO 13485-aligned biomanufacturing workflows.

Key Features

• Microprocessor-based PID temperature control with real-time feedback loop and environmental scanning logic for adaptive thermal regulation
• Large backlit LCD display showing setpoints and actual values for temperature, speed, and remaining time—enabling immediate operational verification
• Parameter lock function to prevent unauthorized modification of critical settings during unattended operation
• Non-volatile memory retains user-defined protocols—including speed, temperature, and timer—even after power interruption
• Gradual acceleration circuitry ensures smooth ramp-up to target rpm, minimizing shear stress on sensitive cells or fragile aggregates
• Integrated refrigeration system with manual cooling setpoint adjustment—capable of sub-ambient operation down to 4 °C without external chillers
• AC induction motor with brushless design, constant torque output, and wide-speed range (30–300 rpm, ±1 rpm accuracy)
• Acoustic and visual alarm system triggered by overtemperature, motor overheating, or system fault—automatically cutting power to affected subsystems
• Auto-restart functionality upon power recovery, resuming prior operation sequence without manual reconfiguration
• Electrostatically coated steel enclosure with tempered glass viewing window and ergonomic top-hinged door for safe, unobstructed access

Sample Compatibility & Compliance

The ZWY-211E supports standard laboratory glassware and bioreactor-compatible vessels including Erlenmeyer flasks (100–5000 mL), baffled culture bottles, and disposable bioreactor bags (with appropriate mounting fixtures). Its uniform airflow pattern and consistent orbital dynamics ensure minimal stratification and optimal mass transfer—critical for aerobic cultivations governed by ASTM D5210 (standard test method for determining aerobic biodegradation) and USP <1043> (cell culture performance qualification). The device complies with IEC 61010-1 safety standards for laboratory equipment and incorporates electrical isolation and thermal cutoffs aligned with EN 60335-1. While not inherently 21 CFR Part 11 compliant, its parameter logging capability (via optional RS485/USB interface) supports audit-ready data capture when integrated into validated LIMS or ELN environments under GxP frameworks.

Software & Data Management

Standard operation requires no external software—the onboard controller manages all core functions autonomously. For advanced integration, the ZWY-211E features an RS485 serial port (Modbus RTU protocol) and optional USB-to-serial adapter for connection to PC-based monitoring platforms. Third-party SCADA or LabVIEW applications can log temperature, speed, and runtime data at user-defined intervals. All alarm events—including overtemperature triggers and power loss—are timestamped in non-volatile memory and retrievable via serial query. When deployed in regulated environments, users may implement supplementary electronic signatures and change-control procedures using validated middleware to satisfy FDA 21 CFR Part 11 requirements for electronic records and signatures.

Applications

• High-density bacterial culture (e.g., E. coli, Bacillus subtilis) for plasmid amplification and recombinant enzyme production
• Mammalian and insect cell suspension culture in serum-free or chemically defined media
• Fungal and yeast fermentation for metabolite screening and secondary compound synthesis
• Enzyme kinetics assays requiring simultaneous thermal and agitation control
• Environmental microbiology studies—including bioremediation strain adaptation and wastewater treatment microcosms
• Pharmaceutical stability testing per ICH Q1A(R2) guidelines under controlled agitation and temperature profiles
• Preparation of competent cells, phage lysates, and antigenic preparations in diagnostic reagent manufacturing

FAQ

What is the minimum operating temperature, and does it support refrigerated incubation?
The ZWY-211E achieves a minimum setpoint of 4 °C using its built-in refrigeration module—enabling low-temperature culturing of psychrophilic organisms or cold-sensitive protein expression systems.
Can the shaker maintain temperature uniformity across all positions on the platform?
Yes—forced convection airflow and optimized baffle geometry ensure temperature uniformity ≤±1 °C across the full shelf area at 37 °C, verified per ISO 17025-accredited calibration protocols.
Is the orbital motion truly circular, and how is eccentricity controlled?
The drive mechanism employs a precision-machined cam-and-linkage assembly producing true circular motion with a fixed Φ26 mm orbit diameter—measured and certified during factory acceptance testing.
Does the unit include validation documentation for GMP environments?
Factory-installed IQ/OQ documentation is available upon request; PQ execution requires site-specific sensor mapping and protocol development per Annex 15 of EU GMP Guidelines.
What maintenance is required for long-term reliability?
The brushless AC motor and sealed refrigeration circuit require no scheduled maintenance; periodic cleaning of air intake filters and verification of door gasket integrity are recommended every six months.