ZHWY ZWF-1112 Dual-Layer Reciprocating Large-Capacity Incubator Shaker

Overview

The ZHWY ZWF-1112 Dual-Layer Reciprocating Large-Capacity Incubator Shaker is an engineered platform for precise, scalable, and reproducible temperature-controlled agitation in life science and biopharmaceutical applications. Designed around a robust dual-layer vertical architecture, it integrates reciprocating linear orbital motion with forced-air convection and high-fidelity PID temperature regulation—enabling uniform thermal distribution and mechanical consistency across large-volume cultures. Its operational principle relies on track-driven oscillation that generates controlled shear and mixing profiles ideal for aerobic microbial growth, suspension cell culture, enzymatic assays, and fermentation process development. The system meets the spatial efficiency demands of modern core facilities while supporting GMP-aligned workflows through hardware-level safety interlocks and deterministic control logic.

Key Features

• Dual-layer vertical design maximizes usable chamber volume (543 L) within a compact footprint (1270 × 820 mm base), optimizing floor space in regulated laboratory environments.
• Reciprocating linear orbital motion (30–240 rpm, ±1 rpm accuracy) with continuously adjustable stroke amplitude (0–50 mm) ensures compatibility with diverse vessel geometries—from test tubes to 1 L baffled flasks—without vortexing or splashing.
• PID microprocessor controller with environmental scanning capability delivers exceptional thermal stability (≤ ±0.1 °C at 37 °C) and uniformity (≤ ±1.0 °C at 37 °C) across both shelves simultaneously.
• LCD backlit display presents real-time and setpoint values for temperature, speed, stroke, and remaining time; all parameters are password-locked to prevent unauthorized modification.
• Integrated safety architecture includes over-temperature cut-off, motor overheat protection, power-failure recovery, and soft-start acceleration to safeguard sensitive biological samples during startup and shutdown cycles.
• Electrostatically coated steel chamber with reinforced tempered glass viewing window supports long-term corrosion resistance and visual monitoring without compromising structural integrity or thermal insulation.

Sample Compatibility & Compliance

The ZWF-1112 accommodates standard laboratory vessels up to 1000 mL per position, supporting configurations such as 44 × 1000 mL flasks, 76 × 500 mL Erlenmeyer flasks, or 164 × 100 mL culture tubes—ideal for upstream bioprocess scale-up and QC batch testing. Its temperature range (ambient +5 to 60 °C) and stable operation within ISO Class 5–7 cleanroom ambient conditions (5–25 °C) align with common requirements for mammalian cell expansion, bacterial fermentation, and diagnostic reagent production. While not certified to specific regulatory standards out-of-the-box, the instrument’s deterministic behavior, parameter logging capability, and hardware-enforced access controls provide foundational support for validation under FDA 21 CFR Part 11, EU Annex 11, and GLP/GMP quality systems when integrated with compliant data management software.

Software & Data Management

The embedded controller stores up to 10 user-defined protocols with full recall functionality, eliminating repetitive manual entry during routine operations. Though standalone operation is fully functional, optional RS-485 or USB interfaces enable integration with third-party laboratory information management systems (LIMS) or SCADA platforms for centralized monitoring and audit-trail generation. All timer events—including end-of-run alarms with audible and visual alerts—are logged with timestamps. For regulated environments, external data acquisition modules may be deployed to capture continuous temperature and speed traces, satisfying traceability requirements under ISO/IEC 17025 or USP .

Applications

This shaker serves critical roles in academic research, clinical diagnostics, and industrial biomanufacturing. Typical use cases include aerobic cultivation of E. coli, S. cerevisiae, and Bacillus strains; suspension culture of CHO and HEK293 cells; enzyme kinetics studies requiring precise thermal and kinetic control; and stability testing of therapeutic proteins under accelerated conditions. Its dual-shelf configuration facilitates comparative experiments—e.g., parallel optimization of media formulations or oxygen transfer rates—while its large capacity enables pilot-scale fermentation runs compatible with downstream purification workflows.

FAQ

What is the maximum flask size supported on each shelf?
Each 940 × 580 mm shelf accommodates up to 44 × 1000 mL baffled flasks or equivalent volumetric configurations, verified under nominal load and 150 rpm operation.
Does the unit support refrigerated operation below ambient temperature?
No—the ZWF-1112 is a heating-only incubator shaker with a minimum operating temperature of ambient +5 °C; active cooling is not integrated.
Is calibration documentation provided with the instrument?
Factory calibration certificates for temperature and speed sensors are available upon request and may be supplemented with IQ/OQ documentation packages for GxP environments.
Can the shaker operate continuously for extended durations?
Yes—designed for unattended 24/7 operation, the brushless AC induction motor and thermal management system ensure reliability over 500-hour timed runs and beyond.
How is user access controlled to prevent parameter tampering?
All operational parameters are protected by a configurable numeric password; only authorized personnel can modify settings or clear alarm states.