ZOLIX Holospec/EMvision High-Sensitivity Fiber-Coupled Raman Spectrometer
| Brand | ZOLIX |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | OEM Manufacturer |
| Instrument Type | Grating-Based Raman Spectrometer |
| Model | Holospec/EMvision |
| Price | USD 100,000 |
| Spectral Range | 532–1100 nm |
| Spectral Resolution | 6.5 cm⁻¹ |
| Minimum Wavenumber | 100 cm⁻¹ |
| Spectral Repeatability | ±0.1 cm⁻¹ |
Overview
The ZOLIX Holospec/EMvision is a high-sensitivity, fiber-coupled Raman spectrometer engineered for demanding analytical applications requiring low-light detection, spatially resolved spectroscopy, and real-time spectral acquisition. Built around a transmission-volume phase holographic grating (VPHG) optical architecture, the Holospec core spectrometer achieves an f/1.8 throughput—significantly higher than conventional Czerny-Turner or crossed-beam designs—enabling superior photon collection efficiency in the visible to near-infrared range (532–1100 nm). This design minimizes stray light and preserves etendue, making it especially suitable for coupling with external fiber-optic Raman probes where signal attenuation is critical. The system operates with a calibrated wavenumber range extending from 100 cm⁻¹, ensuring robust detection of low-frequency lattice modes and broad-band molecular vibrations. Its spectral repeatability of ±0.1 cm⁻¹ supports quantitative long-term monitoring under GLP-compliant laboratory conditions.
Key Features
- Transmission VPHG optical engine optimized for maximum étendue preservation and minimal polarization dependence
- f/1.8 input optics enabling >3× higher radiant throughput compared to standard f/4 spectrographs
- Integrated thermoelectric cooling (−15 °C typical operating temperature) for dark-current suppression and extended integration times
- Modular interface supporting SMA905, FC/PC, and custom fiber pigtails—compatible with OEM probe integration
- Real-time spectral acquisition at up to 30 spectra per second (full-frame, 2048-pixel CCD readout)
- Factory-calibrated wavelength and intensity response traceable to NIST-traceable standards
Sample Compatibility & Compliance
The Holospec/EMvision platform supports both free-space and fiber-coupled sampling configurations. When paired with the optional EMvision micro-endoscopic Raman probe—featuring sub-1.2 mm outer diameter, integrated white-light LED illumination, and coaxial CMOS imaging—the system enables in vivo tissue interrogation during clinical endoscopy procedures. The probe’s 20× microscope objective delivers diffraction-limited spatial resolution (~1.5 µm lateral, ~5 µm axial at 785 nm), while its dual-channel architecture permits simultaneous structural imaging and Raman spectral acquisition. All optical components comply with ISO 13485:2016 requirements for medical device manufacturing. Firmware and data handling protocols support audit trails, electronic signatures, and metadata embedding in accordance with FDA 21 CFR Part 11 and EU Annex 11 guidelines for regulated environments.
Software & Data Management
The system ships with ZOLIX SpectraSuite Pro—a modular, API-accessible software suite supporting spectral acquisition, multivariate analysis (PCA, PLS-DA), spectral library matching (NIST Raman Database, custom libraries), and real-time baseline correction (Asymmetric Least Squares). Raw data is stored in HDF5 format with embedded calibration metadata, instrument configuration logs, and user-defined annotations. Export options include CSV, JCAMP-DX, and mzML-compatible formats for interoperability with third-party chemometrics platforms (e.g., MATLAB, Python scikit-learn, Unscrambler X). Remote operation via TCP/IP and RESTful API enables integration into automated QA/QC workflows and centralized LIMS architectures.
Applications
- Pharmaceutical solid-state characterization (polymorph identification, API-excipient interaction mapping)
- Intraoperative tumor margin assessment during endoscopic resection (e.g., gastrointestinal, bronchial, urological cancers)
- Microplastic identification and polymer classification in environmental water and sediment samples
- Carbon nanomaterial quality control (G/D ratio quantification, defect density profiling)
- Reaction monitoring in catalytic flow reactors using immersion fiber probes
- Forensic trace evidence analysis (paint chips, textile fibers, illicit drug mixtures)
FAQ
What excitation wavelengths are supported by the Holospec/EMvision system?
The spectrometer is optimized for 532 nm, 660 nm, and 785 nm laser sources; optional versions accommodate 473 nm with UV-grade optics and anti-reflection coatings.
Can the system be used with non-ZOLIX fiber probes?
Yes—standard SMA905 or FC/PC connectors allow integration with third-party Raman probes, provided numerical aperture and core diameter fall within the specified input étendue envelope (NA ≤ 0.22, core ≥ 50 µm).
Is spectral calibration performed at the factory or required on-site?
Each unit undergoes full wavelength and intensity calibration at the Beijing production facility using certified reference standards; users may perform optional verification using built-in neon/argon lamp modules.
Does the EMvision endoscopic probe meet biocompatibility requirements for human use?
The probe housing materials (medical-grade stainless steel, silicone-coated fiber bundle) are USP Class VI certified; however, clinical deployment requires local IRB approval and adherence to IEC 60601-1 safety standards.
How is spectral stability maintained over time and temperature fluctuations?
The optical bench employs low-expansion Invar mounting and active thermal stabilization; spectral drift is <0.05 cm⁻¹ over 8 hours at ambient ±2 °C variation.
