ZWY-C2102 Dual-Layer Programmable Temperature- and Humidity-Controlled Orbital Shaker

Overview

The ZWY-C2102 Dual-Layer Programmable Temperature- and Humidity-Controlled Orbital Shaker is an engineered platform for precise, reproducible cultivation of microbial, mammalian, and hybridoma cultures under dynamically controlled environmental conditions. Designed around a closed-loop PID control architecture with real-time environmental scanning microprocessing, the instrument maintains tight regulation of temperature (4–60 °C), relative humidity (45–90 %RH), and orbital speed (30–240 rpm) across two independently accessible chamber layers. Its forced convection airflow system ensures uniform thermal and hygric distribution—critical for avoiding localized desiccation or condensation in long-duration incubations. Unlike basic shakers, the ZWY-C2102 integrates active humidification via a patented internal micro-humidifier that delivers stable vapor dispersion without water pooling or aerosol carryover—enabling reliable maintenance of moisture-sensitive samples such as primary cell suspensions, tissue explants, and low-volume bioreactor cultures.

Key Features

• Dual-layer chamber architecture (380 L total volume) with two removable stainless-steel electro-polished shelves (734 × 458 mm each), supporting scalable parallel experiments without cross-contamination.
• 6-segment × 12-step programmable logic enabling complex multi-phase protocols—including temperature ramping, humidity stepping, speed profiling, and cyclic recovery phases—each segment configurable up to 100 hours.
• Integrated water-level monitoring with automatic refill function and dual-stage audible/visual low-water alarms, eliminating manual top-up during unattended overnight or multi-day runs.
• Multi-tiered safety architecture: independent adjustable and fixed overtemperature cutouts, refrigeration overload protection, ground-fault circuit interruption (GFCI), electronic overcurrent tripping, and door-interlocked operation.
• Brushless AC induction motor with constant-torque output across full speed range; zero-maintenance design with no carbon brushes or gear wear, ensuring long-term rotational stability and minimal vibration transmission.
• Backlit LCD interface displaying real-time setpoints and actual values for temperature, humidity, speed, elapsed time, and system status—including diagnostic codes for sensor validation and fault isolation.

Sample Compatibility & Compliance

The ZWY-C2102 accommodates standard culture vessels including Erlenmeyer flasks (up to 5 L), deep-well plates, roller bottles, and custom bioreactor cartridges—secured via spring-loaded clamps or optional detachable platforms. Its stainless-steel interior (electropolished grade 316L), non-porous glass viewport, and corrosion-resistant structural components meet ISO 14644-1 Class 8 cleanroom compatibility requirements for controlled environment labs. The device supports GLP-compliant workflows through parameter encryption, audit-trail-capable RS-232 data logging (with timestamped event records), and user-access-level configuration controls. While not certified to IEC 61000-4 electromagnetic immunity standards out-of-box, its grounded metal chassis and shielded internal cabling provide robust noise rejection in shared laboratory infrastructure.

Software & Data Management

Data acquisition and remote supervision are enabled via the built-in RS-232 serial interface, compatible with industry-standard terminal emulation software (e.g., Tera Term, PuTTY) and LabVIEW-compatible drivers. All operational parameters—including setpoints, real-time sensor outputs, alarm triggers, and power-event timestamps—are streamable in ASCII format at user-defined intervals. An embedded thermal printer provides hard-copy documentation of startup sequences, calibration logs, and end-of-run summaries—essential for FDA 21 CFR Part 11–aligned record retention where electronic signatures are not yet deployed. Parameter memory retains up to 100 user-defined programs with password-protected modification rights, and all settings persist through power cycling or scheduled shutdowns.

Applications

This shaker serves critical roles in upstream bioprocess development, including aerobic bacterial fermentation (e.g., E. coli, Bacillus subtilis), yeast propagation, insect cell line expansion (Sf9, High Five), and suspension-adapted CHO cultures. Its humidity control capability extends utility to plant tissue culture, fungal sporulation studies, and enzymatic hydrolysis assays requiring minimized evaporation loss. In pharmaceutical QC laboratories, it supports accelerated stability testing per ICH Q1A(R3) guidelines when configured with defined temperature/humidity stress profiles. Academic research applications span synthetic biology construct validation, microbiome community dynamics under controlled moisture gradients, and biomaterial scaffold seeding under physiologically relevant oscillatory shear.

FAQ

Does the ZWY-C2102 support CO₂ concentration control?
No—CO₂ regulation is exclusive to the ZWY-D series models. The C2102 is optimized for temperature and humidity precision only.
Can the unit operate continuously for >72 hours without intervention?
Yes—its auto-refill humidification system, refrigeration thermal management, and power-fail recovery ensure uninterrupted operation across multi-day protocols.
Is the internal chamber volume usable for stacked vessel configurations?
Yes—the 800 mm vertical clearance between shelves permits upright placement of 3 L flasks on both levels simultaneously.
What calibration documentation is provided with shipment?
Each unit ships with a factory-verified NIST-traceable temperature and humidity calibration certificate covering three points across the operational range.
Are third-party data acquisition systems supported beyond RS-232?
RS-485 and Ethernet-to-serial gateways may be integrated externally; however, native TCP/IP or USB connectivity is not available on this model.