ZWF-3312B Dual-Layer Reciprocating Orbital Shaker

Overview

The ZWF-3312B Dual-Layer Reciprocating Orbital Shaker is an industrial-grade laboratory shaker engineered for high-throughput cell culture, microbial fermentation, and biochemical reaction support under controlled ambient conditions. Designed for integration into centralized incubation environments—such as walk-in climate chambers or temperature-stabilized production rooms—it delivers synchronized, low-shear orbital motion across two independently usable platforms. Unlike rotary or vortex shakers, this model employs a cam-driven reciprocating track mechanism to generate precise, repeatable horizontal orbital displacement (0–50 mm adjustable amplitude) at speeds ranging from 30 to 300 rpm. Its PID-controlled microprocessor continuously monitors and compensates for load-induced torque fluctuations, ensuring stable rotational velocity and minimal mechanical drift over extended operation cycles (up to 500 hours). The unit operates via natural convection cooling and requires no forced-air jacketing, making it compatible with standard ISO Class 5–7 cleanroom-compatible enclosures and modular bioprocess suites.

Key Features

• Two-tier stainless-steel-reinforced aluminum alloy trays (800 × 600 mm each), precision-machined in one-piece casting for long-term dimensional stability and vibration damping
• Brushless AC induction motor with constant-torque, wide-range speed regulation—no carbon brushes, zero routine maintenance, rated for >20,000 hours MTBF
• Soft-start acceleration profile prevents sample splashing and ensures gentle ramp-up of orbital motion, critical for suspension cultures and fragile recombinant protein expression systems
• Backlit LCD interface displays real-time speed, elapsed time, remaining timer, and setpoint parameters; supports multi-user access control via encrypted parameter locking
• Power failure recovery function automatically resumes pre-interruption settings—including speed, orbit amplitude, and remaining timer—upon grid restoration, compliant with GLP audit trail requirements
• Electrostatically sprayed powder-coated steel chassis (RAL 7035 finish) provides corrosion resistance and structural rigidity; footprint optimized for benchtop-to-floor transition in GMP-compliant facilities

Sample Compatibility & Compliance

The ZWF-3312B accommodates standard Erlenmeyer flasks (up to 5 L), Fernbach bottles, roller bottles, and custom bioreactor vessels secured via universal clamping kits or silicone matting. Its dual-tray configuration enables parallel processing of heterogeneous sample sets—for instance, comparative fermentation trials across strain variants or media formulations—without cross-contamination risk. The device meets IEC 61000-6-2 (EMC immunity) and IEC 61000-6-4 (EMC emission) standards. While not intrinsically rated for hazardous locations, its electrical design conforms to IEC 61010-1 for laboratory equipment safety. When operated within validated environmental chambers, the system supports protocols aligned with USP , ISO 13408-1 (aseptic processing), and ASTM E2500-13 (verification of laboratory equipment).

Software & Data Management

This shaker operates as a standalone instrument with embedded firmware—no external PC dependency required for basic operation. All operational parameters are stored in non-volatile memory with timestamped change logs accessible via service mode. Optional RS-485 Modbus RTU interface (available upon request) enables integration into SCADA or MES platforms for remote monitoring, batch record generation, and electronic signature-enabled deviation reporting per FDA 21 CFR Part 11 Annex 11 requirements. Audit trails include operator ID (if configured), parameter modification events, runtime anomalies, and power cycle history—exportable in CSV format for quality review.

Applications

The ZWF-3312B serves as a core platform in upstream bioprocessing workflows, including aerobic bacterial cultivation (e.g., E. coli, Bacillus subtilis), yeast propagation (S. cerevisiae, P. pastoris), mammalian suspension culture (CHO, HEK293), and enzymatic hydrolysis kinetics studies. It is routinely deployed in QC/QA labs for media performance qualification, sterility test incubation support, and reference standard preparation per EP 2.6.27 and JP XVII guidelines. Additional use cases include environmental microbiology (water/biofilm sampling analysis), food safety validation (pathogen enrichment), and academic research in synthetic biology and metabolic engineering where reproducible aeration and mixing efficiency directly impact titer consistency.

FAQ

What is the maximum flask capacity supported per tray?
Each 800 × 600 mm tray accommodates up to twelve 2 L Erlenmeyer flasks (with proper balancing) or eight 3 L vessels using optional heavy-duty clamps.
Is the orbit diameter calibrated and traceable to national standards?
Orbit amplitude is factory-set using laser-interferometric verification; calibration certificates (NIST-traceable) are available as an add-on service.
Can the shaker be installed inside a CO₂ incubator?
No—this model lacks sealed electronics or humidity-resistant components; it is intended for ambient-temperature, low-humidity environments only.
Does the unit support continuous operation beyond 500 hours?
Yes—timer auto-reset functionality allows indefinite cycling; the 500 h limit applies only to single-programmed intervals.
Are replacement trays and drive belts available as spare parts?
All mechanical subassemblies—including cam followers, timing belts, and tray mounting brackets—are stocked globally and supplied with full OEM documentation and RoHS compliance statements.