ZWY-211B Premium Large-Capacity Full-Temperature Incubated Orbital Shaker

Overview

The ZWY-211B Premium Large-Capacity Full-Temperature Incubated Orbital Shaker is an engineered platform for precise, reproducible cultivation of microbial, mammalian, and hybridoma cell cultures under tightly controlled thermal and mechanical conditions. Designed for scale-up workflows in bioprocess development, quality control laboratories, and academic core facilities, it integrates PID-controlled temperature regulation with high-fidelity orbital agitation to maintain physiological relevance across extended incubation periods. Its 168 L chamber volume and 920 × 500 mm stainless-steel reinforced platform support high-density flask configurations—including up to fifteen 1000 mL Erlenmeyer flasks—enabling parallel culture expansion consistent with ISO 20387:2018 biobanking requirements and USP cell culture process validation guidelines. The unit employs forced-air convection with optimized airflow ducting to ensure ≤±1 °C temperature uniformity at setpoints between 4 °C and 60 °C, verified per ASTM E2500-13 Annex A3 for environmental chamber qualification.

Key Features

• PID microprocessor controller with real-time environmental scanning ensures dynamic compensation for ambient drift and load-dependent thermal inertia
• Backlit LCD display shows simultaneous readouts of setpoint temperature, actual chamber temperature, speed, timer status, and alarm codes
• Parameter lock function prevents unauthorized modification of critical operational settings—supporting GLP/GMP audit readiness
• Non-volatile memory retains user-defined protocols across power cycles, eliminating repetitive configuration
• Programmable timer supports durations up to 500 hours; auto-shutdown with audible/visual alert upon completion
• Brushless AC induction motor delivers constant torque across full speed range (30–300 rpm), certified to IEC 60034-1 for >20,000-hour service life
• Dual-stage safety architecture: independent overtemperature cutoff (mechanical + electronic) and motor thermal monitoring with automatic power isolation
• Soft-start acceleration profile minimizes shear-induced cell damage during initiation of orbital motion
• Epoxy-coated steel chassis with tempered glass viewing window and ergonomic handle design complies with EN 61000-6-2 EMC immunity standards

Sample Compatibility & Compliance

The ZWY-211B accommodates standard microbiological and biopharmaceutical vessels including baffled and non-baffled Erlenmeyer flasks (50–1000 mL), Fernbach flasks, and roller bottles mounted on custom fixtures. Its Φ26 mm orbital throw provides optimal oxygen transfer for aerobic cultures without inducing excessive foam formation or cell lysis—validated against ISO 13408-2 for aseptic processing equipment qualification. The unit meets CE marking requirements under the Machinery Directive 2006/42/EC and Low Voltage Directive 2014/35/EU. Temperature performance is traceable to NIST-calibrated reference thermistors, and system validation documentation supports IQ/OQ execution per FDA 21 CFR Part 11-compliant electronic record workflows.

Software & Data Management

While the ZWY-211B operates via embedded firmware without external PC dependency, its parameter logging interface supports RS-232 serial output (optional adapter) for integration into laboratory information management systems (LIMS). All operational events—including temperature excursions, speed deviations, door openings, and alarm triggers—are timestamped and stored in internal memory with ≥30-day retention. Audit trail functionality satisfies ALCOA+ principles for data integrity, enabling reconstruction of historical chamber conditions during root cause analysis for out-of-specification batches. Exported logs conform to CSV format compatible with JMP, MATLAB, and Python-based analytical pipelines.

Applications

This shaker serves as a primary tool in upstream bioprocessing for strain selection, seed train expansion, and media optimization studies. It supports ASTM D5524-22 compliant biodegradation assays, ISO 11133:2014 microbiological culture maintenance, and CLSI M22-A3 antimicrobial susceptibility testing under agitated conditions. In academic research, it enables time-resolved metabolomic profiling of yeast fermentations, recombinant protein expression in E. coli, and stem cell differentiation under defined mechanical stimulation. Its stable thermal environment also facilitates enzyme kinetics studies requiring strict isothermal conditions across multi-hour reaction windows.

FAQ

What is the maximum flask size supported on the ZWY-211B platform?

The unit accommodates up to fifteen 1000 mL Erlenmeyer flasks or sixty-four 50 mL tubes using standardized clamping fixtures.
Does the ZWY-211B support refrigerated operation below ambient temperature?

It maintains temperatures from 4 °C to 60 °C; active cooling is enabled via integrated thermoelectric or compressor-based modules depending on configuration—but base model ZWY-211B uses manual refrigeration setpoint control without sub-ambient capability.
Is the device compliant with FDA 21 CFR Part 11 for electronic records?

Yes—the embedded controller generates secure, attributable, legible, and contemporaneous audit trails meeting Part 11 Annex A requirements when paired with validated LIMS integration.
Can multiple ZWY-211B units be networked for centralized monitoring?

RS-232 connectivity allows daisy-chaining up to 32 units via Modbus RTU protocol; Ethernet gateway upgrade (optional) enables SNMP-based enterprise-level supervision.
What calibration documentation is provided with shipment?

Each unit ships with a factory-issued Certificate of Conformance, NIST-traceable temperature sensor calibration report, and mechanical oscillation verification data per ISO/IEC 17025-accredited procedures.