ZWY-C2112D Dual-Layer Programmable Temperature- and Humidity-Controlled Orbital Shaker

Overview

The ZWY-C2112D Dual-Layer Programmable Temperature- and Humidity-Controlled Orbital Shaker is an engineered platform for precision-controlled microbial cultivation, enzymatic assays, cell-based bioprocessing, and long-term biochemical incubation under dynamically regulated environmental conditions. Designed around a dual-chamber orbital architecture, it integrates P.I.D.-based microprocessor control with real-time environmental scanning to maintain stable, repeatable setpoints across temperature (4–60 °C), relative humidity (45–90 %RH), and rotational speed (30–300 rpm). Its forced-air convection system ensures uniform thermal distribution (≤±1 °C at 37 °C), while the patented integrated ultrasonic humidifier—coupled with automatic water-level sensing and refill—prevents sample desiccation during extended runs. The shaker operates on a robust AC induction motor drive, delivering constant torque and speed stability without carbon brushes or routine maintenance. Its dual-layer configuration (two independently accessible shelves, each 970 × 560 mm) supports high-throughput workflows in academic labs, QC facilities, and GMP-aligned biomanufacturing environments.

Key Features

• Dual-layer orbital design with two electro-polished stainless steel shelves (970 × 560 mm each) and full-height tempered glass viewport for non-invasive process monitoring
• Advanced 6-segment × 12-step programmable logic enabling complex protocols: temperature/humidity ramping, stepwise oscillation profiles, cyclic incubation, and hold-and-recover sequences
• Integrated RS-232 interface compliant with ASTM E2500-13 and ISO/IEC 17025 data traceability requirements; supports external logging and remote parameter validation
• Triple-tier safety architecture: hardware-independent overtemperature cutoff (adjustable), independent over-speed limiter, and redundant leak/overcurrent protection meeting IEC 61010-1 Class II safety standards
• Frost-free, R134a-based refrigeration system with adaptive power modulation—ensures consistent cooling performance across ambient conditions (5–35 °C)
• Self-diagnostic microcontroller with audible/visual alarms for sensor failure, low water level, power interruption, and deviation from setpoint thresholds
• Auto-recovery function preserves experiment integrity after grid outage—resumes operation at original parameters including elapsed time and phase position

Sample Compatibility & Compliance

The ZWY-C2112D accommodates standard microbiological vessels (Erlenmeyer flasks up to 5 L, culture bottles, multi-well plates) via interchangeable, detachable tray configurations—including spring-loaded clamps, universal bottle holders, and flat-platform inserts. Its chamber volume (624 L) and internal dimensions (1106 × 665 × 850 mm) allow simultaneous processing of heterogeneous sample sets under identical environmental constraints. The unit conforms to key regulatory expectations for laboratory equipment used in GLP and GMP settings: temperature and humidity sensors are calibrated per ISO/IEC 17025 procedures; all firmware logs include timestamped audit trails supporting FDA 21 CFR Part 11 compliance when paired with validated third-party data acquisition software. Humidity control meets USP criteria for moisture-sensitive biological formulations, and thermal uniformity adheres to ISO 15189 Annex A.3.2 for clinical laboratory instrumentation.

Software & Data Management

While the ZWY-C2112D operates autonomously via its embedded LCD interface (backlit, 128 × 64 pixel resolution), its RS-232 port enables bidirectional communication with host PCs running Windows-based configuration utilities. These tools permit offline protocol development, batch parameter upload/download, and export of time-stamped CSV logs containing temperature, humidity, speed, and alarm status. The built-in micro printer provides immediate hard-copy output of start/end timestamps, current setpoints, and deviation alerts—serving as primary evidence for SOP adherence during internal audits. All user-modifiable parameters (e.g., PID coefficients, alarm thresholds, calibration offsets) are password-protected and retained in non-volatile memory, ensuring data integrity across power cycles and firmware updates.

Applications

This shaker serves critical roles in applied life science research and quality control laboratories. It supports aerobic bacterial growth studies (e.g., Escherichia coli, Bacillus subtilis) requiring precise O₂ transfer and thermal consistency; yeast fermentation optimization under controlled hygrometric stress; hybridoma cell line expansion for monoclonal antibody production; and enzyme kinetic assays where hydration state directly influences catalytic efficiency. In pharmaceutical development, it facilitates stability testing of lyophilized proteins under ICH Q1A(R3) accelerated conditions (40 °C / 75 %RH). Environmental labs use it for soil microbiome enrichment under simulated field moisture regimes, while food safety units deploy it for pathogen enrichment per AOAC 999.06 and ISO 6579 protocols.

FAQ

Does the ZWY-C2112D support CO₂ control?
No—this model (C-series) lacks integrated CO₂ regulation. For CO₂-enabled applications, refer to the ZWY-D2112D variant.
Is calibration documentation provided with shipment?
Yes—each unit ships with a factory calibration certificate covering temperature, humidity, and speed sensors, traceable to NIM (National Institute of Metrology, China) standards.
Can the humidifier operate continuously for 72+ hours without manual intervention?
Yes—when connected to a continuous water supply via optional external reservoir kit (part #ZWY-HR2), the auto-refill system sustains uninterrupted operation.
What is the maximum flask size supported on each shelf?
Standard configuration accommodates up to four 5 L Erlenmeyer flasks per shelf using reinforced dual-arm clamps.
Is the RS-232 interface compatible with LabVIEW and Python-based control systems?
Yes—the ASCII command protocol is fully documented in the technical manual and supports SCPI-style syntax for integration into custom automation frameworks.