ZWY-C2112B Dual-Layer Programmable Temperature- and Humidity-Controlled Orbital Shaker

Overview

The ZWY-C2112B Dual-Layer Programmable Temperature- and Humidity-Controlled Orbital Shaker is an advanced incubation shaker engineered for precision-controlled microbial cultivation, enzymatic assays, cell suspension culture, and biochemical reaction kinetics under dynamically regulated environmental conditions. It employs PID-controlled microprocessor-based environmental scanning technology to simultaneously regulate temperature (4–60 °C), relative humidity (45–90 %RH), and orbital shaking frequency (30–300 rpm) across two independently accessible stainless-steel shelves. Its forced-air convection system ensures uniform thermal distribution (±1 °C at 37 °C), while the patented internal humidification module—combined with automatic water-level monitoring and replenishment—prevents sample desiccation during extended incubation cycles. Designed for GLP-compliant laboratories, the unit integrates fail-safe thermal, mechanical, and electrical safeguards aligned with IEC 61010-1 safety standards for laboratory equipment.

Key Features

• Dual-layer architecture with two removable 970 × 560 mm electropolished stainless-steel trays—each supporting up to 12 × 1 L Erlenmeyer flasks or equivalent vessel configurations
• 6-segment × 12-step programmable control logic enabling complex multi-stage protocols: temperature ramping, humidity stepping, speed profiling, cyclic repetition, and hold-to-target modes
• High-resolution LCD interface with backlight display showing real-time setpoints and measured values for temperature, humidity, rpm, and elapsed time
• Intelligent water management system featuring auto-fill capability and dual-stage low-water alarm (audible + visual)
• Triple-layer safety architecture: personnel protection (leakage current cutoff, overcurrent trip), sample integrity (independent overtemperature limiter, adjustable upper/lower thermal thresholds), and equipment durability (compressor overload protection, frost-free refrigeration using R134a)
• Brushless AC induction motor delivering consistent torque across full speed range (30–300 rpm ±1 rpm), with soft-start acceleration to minimize shear stress on sensitive cultures
• RS-232 serial interface for external data logging and remote parameter synchronization; embedded thermal printer supports immediate hardcopy of runtime logs and alarm events

Sample Compatibility & Compliance

The ZWY-C2112B accommodates standard microbiological and bioprocess vessels—including baffled flasks (50–2000 mL), culture tubes, microplates (with optional adapter trays), and custom bioreactor sleeves—within its 624 L total chamber volume (interior: 1106 × 665 × 850 mm). Its stainless-steel interior (electropolished grade 316), tempered glass observation window, and static-dissipative powder-coated exterior meet ISO 14644-1 Class 8 cleanroom compatibility requirements for non-sterile but controlled environments. All operational parameters—including temperature calibration offsets, humidity correction factors, and user-access levels—are password-protected and retain settings after power interruption. The system supports audit-trail-ready operation when interfaced with compliant LIMS or ELN platforms, fulfilling foundational requirements for FDA 21 CFR Part 11 adherence in regulated QC/QA workflows.

Software & Data Management

While the ZWY-C2112B operates autonomously via its onboard microcontroller, its RS-232 port enables integration with third-party laboratory software suites for centralized scheduling, historical trend analysis, and electronic batch record generation. The embedded printer outputs timestamped records including start/stop times, alarm triggers (e.g., “Low Water Level – 2024-06-12 14:22:07”), and final condition snapshots (T = 36.8 °C, RH = 72.3 %, rpm = 150). Parameter encryption prevents unauthorized modification, and all configuration changes are logged with operator ID and timestamp. Memory retention preserves up to 100 user-defined programs, each supporting up to 100 hours per segment—sufficient for uninterrupted 4-day fermentation runs or circadian rhythm studies.

Applications

This shaker serves critical roles in pharmaceutical stability testing (ICH Q1A–Q5C), recombinant protein expression (E. coli, Pichia pastoris), mammalian cell line expansion (CHO, HEK293), yeast two-hybrid screening, antibiotic susceptibility profiling, and environmental microbiology (e.g., BOD incubation per ASTM D5210). Its precise humidity control makes it suitable for hygroscopic-sensitive assays such as lyophilized enzyme reconstitution studies and fungal sporulation experiments. In academic research, it supports synthetic biology workflows requiring synchronized oscillatory inputs alongside thermal/hygrometric modulation—enabling investigation of gene expression dynamics under physiologically relevant microenvironments.

FAQ

Does the ZWY-C2112B support CO₂ control?
No—CO₂ regulation is exclusive to the ZWY-D series models. This unit is optimized for ambient-air applications requiring only temperature and humidity modulation.
What is the maximum flask size supported per shelf?
Each shelf accommodates twelve 1 L baffled flasks (or equivalent volume distribution) without compromising airflow uniformity or mechanical stability.
Is calibration traceable to national standards?
Yes—temperature and humidity sensors are factory-calibrated against NIST-traceable references; field recalibration procedures are documented in the technical manual and support ISO/IEC 17025-compliant verification protocols.
Can the unit operate continuously for 7+ days?
Yes—designed for unattended long-term operation with redundant safety systems, auto-recovery from power loss, and continuous compressor duty cycling validated per IEC 60068-2-2.
Are replacement parts and service documentation available internationally?
Authorized distributors provide spare humidifier cartridges, water-level sensors, and PID controller modules; full service manuals, wiring diagrams, and firmware update tools are supplied upon registration with the manufacturer’s global support portal.