ZOLIX CC Series Optical Cuvettes (Quartz & Glass)
| Brand | ZOLIX |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Direct Manufacturer |
| Origin Category | Domestic (China) |
| Models | CCQ1–CCQ100, CCQA, CCG1–CCG100 |
| Optical Material | Fused Silica (Quartz) or Optical Glass |
| Wavelength Range | Quartz: 185 nm – 2.5 µm |
| Glass | 360 nm – 800 nm |
| Pathlength Options | 1–100 mm |
| External Dimensions | 45 × 12.4 × (3.4–102.4) mm |
| Quartz Cuvette Transmission | ≥75% @ 200 nm, ≥80% @ 250 nm, ≥85% @ 300 nm |
| Matched Pair Tolerance | ≤0.5% ΔT at 200/250/300 nm |
| Glass Cuvette Transmission | ≥84% @ 420–700 nm |
| Matched Set Tolerance (4 pcs) | ≤0.5% ΔT |
| Fluorescence-Optimized Variant | CCQA (All-Path, Four-Sided Optical Access) |
Overview
ZOLIX CC Series optical cuvettes are precision-engineered sample containment cells designed for quantitative spectrophotometric, fluorometric, and kinetic measurements in UV-Vis-NIR spectroscopy systems. Constructed from high-purity fused silica (quartz) or optical-grade borosilicate glass, each cuvette is manufactured under strict dimensional and optical homogeneity controls to ensure minimal pathlength deviation, low wavefront distortion, and consistent beam transmission. The fundamental operating principle relies on Beer–Lambert law compliance: incident collimated light passes through a defined liquid or solution sample volume, with absorbance (A) calculated as A = ε·c·l, where ε is the molar absorptivity, c is concentration, and l is the precisely calibrated optical pathlength. Accurate pathlength definition—ranging from 1 mm to 100 mm—and material-specific transmission characteristics directly determine measurement sensitivity, dynamic range, and spectral fidelity across analytical workflows.
Key Features
- Precision-machined fused silica (quartz) cuvettes with certified transmission ≥75% at 200 nm, ≥80% at 250 nm, and ≥85% at 300 nm—enabling reliable UV-range quantitation down to deep-UV wavelengths.
- Optically matched quartz cuvette pairs (ΔT ≤ 0.5% at 200/250/300 nm) and sets (4-piece, ΔT ≤ 0.5% across visible range), essential for blank subtraction, kinetic monitoring, and multi-channel comparative assays.
- CCQA variant features four polished optical faces (all-path design), optimized for fluorescence emission collection at 90° geometry and enabling excitation/emission path flexibility in dual-monochromator fluorometers.
- Airtight quartz cuvettes available with O-ring sealed caps for handling volatile solvents, air-sensitive samples, or controlled-atmosphere experiments—compatible with standard spectrophotometer holders and temperature-controlled cuvette jackets.
- Consistent external dimensions (45 × 12.4 mm cross-section) across all pathlengths (1–100 mm) ensure mechanical interchangeability and reproducible positioning in automated sample changers and robotic platforms.
- Glass cuvettes (CCG series) meet ISO 3664 and ASTM E275 specifications for visible-range photometry: ≥84% transmission between 420–700 nm, with matched-set tolerance ≤0.5%—ideal for routine QC in pharmaceutical, food, and environmental labs.
Sample Compatibility & Compliance
ZOLIX cuvettes support aqueous, organic, and mildly corrosive solutions. Quartz variants resist hydrofluoric acid-free solvents, including acetonitrile, methanol, chloroform, and dilute mineral acids (HCl, HNO₃ < 1 M). Glass cuvettes are recommended for non-UV applications involving alkaline buffers or high-pH media where quartz may exhibit surface leaching. All cuvettes comply with GLP-aligned manufacturing documentation protocols, including individual lot traceability, raw material certification (ASTM F795 for fused silica), and optical homogeneity verification per ISO 10110-2. Sealed variants conform to ICH Q5C stability testing requirements for closed-system photostability assessment under ICH Q1B guidelines.
Software & Data Management
While cuvettes are passive optical components, their metrological integrity directly impacts data validity in regulated environments. ZOLIX provides calibration-ready documentation packages—including pathlength verification reports (certified via interferometric measurement), transmission spectra (200–1100 nm), and matched-pair transmission logs—supporting 21 CFR Part 11-compliant instrument qualification (IQ/OQ/PQ) and audit-ready record retention. Integration with LIMS and ELN systems is facilitated through standardized metadata tagging (e.g., lot number, pathlength, material, match ID) compatible with ASTM E1384 and ISO/IEC 17025 digital reporting frameworks.
Applications
- UV-Vis quantitative analysis of nucleic acids, proteins, and small molecules (e.g., A260/A280 ratio determination, enzyme kinetics)
- Fluorescence quantum yield measurements using CCQA four-face cuvettes with orthogonal excitation/detection alignment
- High-pathlength (50–100 mm) trace analyte detection in environmental water testing (e.g., nitrate, phosphate)
- Photostability studies under controlled irradiation (ICH Q1B) using sealed quartz cuvettes
- Reference standard preparation and calibration curve validation in ISO/IEC 17025-accredited laboratories
- Microvolume adaptation via 1–2 mm pathlength cuvettes coupled with beam-focusing optics in microplate-compatible spectrophotometers
FAQ
What wavelength range is supported by ZOLIX quartz cuvettes?
Fused silica cuvettes transmit effectively from 185 nm (deep UV) to 2.5 µm (mid-IR), with guaranteed minimum transmission of 75% at 200 nm, 80% at 250 nm, and 85% at 300 nm.
How are matched cuvette pairs verified?
Each matched pair undergoes spectrophotometric transmission profiling at 200 nm, 250 nm, and 300 nm; the absolute difference in %T at each wavelength must not exceed 0.5%, ensuring baseline consistency for differential measurements.
Can CCQA cuvettes be used for both absorption and fluorescence?
Yes—CCQA’s four polished faces allow simultaneous excitation and emission detection at right angles, while maintaining precise 10 mm pathlength for absorbance reference; it is widely deployed in steady-state and time-resolved fluorometry.
Are custom pathlengths or flow-through configurations available?
ZOLIX offers OEM customization including non-standard pathlengths (e.g., 0.5 mm, 15 mm), integrated PTFE septa, threaded inlet/outlet ports, and temperature-jacketed housings—subject to minimum order quantities and engineering review.
Do glass cuvettes meet international photometric standards?
Yes—CCG series complies with ASTM E275 and ISO 3664 for visible photometry, delivering ≥84% transmission across 420–700 nm with inter-cuvette variability ≤0.5% in matched sets of four.
