INNOTEG SPME Fiber for Solid Phase Microextraction
| Brand | INNOTEG |
|---|---|
| Origin | Guangdong, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | SPME Fiber |
| Pricing | Upon Request |
Overview
The INNOTEG SPME Fiber is a precision-engineered solid-phase microextraction (SPME) consumable designed for integration with standard SPME holders and autosamplers compatible with gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), and liquid chromatography–mass spectrometry (LC–MS) platforms. SPME is a solvent-free, equilibrium-based extraction technique developed in 1990 by Prof. Janusz Pawliszyn at the University of Waterloo. It relies on the partitioning of analytes between a sample matrix (liquid, headspace, or solid) and a thin, coated fused-silica fiber—typically 1 cm or 2 cm in exposed length—immobilized within a stainless-steel needle housing. During operation, the fiber is extended from its protective sheath into the sample environment to adsorb or absorb target compounds via reversible interactions (e.g., van der Waals forces, hydrogen bonding, π–π stacking, or hydrophobic partitioning). After equilibration or timed exposure, the fiber is retracted and thermally desorbed directly in the GC inlet or solvent-eluted for LC analysis. This eliminates liquid handling, reduces contamination risk, and enables trace-level preconcentration with high reproducibility.
Key Features
- Solvent-free extraction methodology compliant with green chemistry principles (reduced waste, no organic solvent consumption)
- Fused-silica fiber core (1 cm or 2 cm active length) with precisely controlled coating thickness and uniformity
- Stainless-steel protective sheath enabling robust mechanical protection and precise fiber extension/retraction
- Interchangeable fiber coatings available across multiple chemistries (e.g., PDMS, CAR/PDMS, DVB/CAR/PDMS, PA, CW/TPR) — compatible with diverse analyte polarities and volatility ranges
- Designed for compatibility with commercial SPME manual holders and automated GC/LC autosamplers (e.g., CTC Analytics, Gerstel, Thermo Fisher, Agilent)
- Batch-tested for consistent coating integrity, thermal stability (up to 250–300 °C depending on phase), and low blank response
Sample Compatibility & Compliance
The INNOTEG SPME Fiber supports direct immersion (DI-SPME) and headspace (HS-SPME) sampling modes for aqueous, semi-solid, gaseous, and biological matrices—including wastewater, drinking water, food homogenates, blood plasma, tissue extracts, and forensic swabs. Its design aligns with widely adopted regulatory frameworks governing analytical method validation and sample preparation: it facilitates adherence to ISO 17025 requirements for traceability and uncertainty estimation; supports GLP-compliant workflows when integrated with audit-trail-enabled autosamplers; and enables method development consistent with USP , EPA Methods 8260/8270, and ASTM D7703 for volatile and semi-volatile organic compound analysis. All fibers undergo rigorous quality control per internal specifications aligned with ICH Q2(R2) guidance for analytical procedure validation.
Software & Data Management
While the SPME fiber itself is a passive consumable, its performance is fully traceable when used within validated instrument control environments. When deployed with GC or GC–MS systems equipped with 21 CFR Part 11–compliant software (e.g., Thermo Chromeleon, Agilent OpenLab CDS, Waters Empower), full electronic records—including fiber lot number, extraction time, temperature, agitation parameters, and desorption conditions—can be captured as metadata. INNOTEG provides batch-specific certificates of conformance and coating specification sheets, supporting laboratory documentation requirements under ISO/IEC 17025 and pharmaceutical quality systems (GMP Annex 11). Optional application notes and method transfer protocols are available upon request to support method validation and inter-laboratory comparability.
Applications
- Environmental monitoring: quantification of BTEX, PAHs, pesticides, and endocrine disruptors in surface water, groundwater, and soil leachates
- Food & flavor analysis: headspace profiling of volatiles in dairy, fruit, coffee, and fermented products
- Forensic toxicology: rapid screening of drugs of abuse (e.g., cannabinoids, opioids, amphetamines) in oral fluid and postmortem blood
- Clinical pharmacokinetics: unbound drug fraction measurement in plasma using equilibrium dialysis–SPME coupling
- Pharmaceutical development: residual solvent analysis in APIs and excipients per ICH Q3C guidelines
- Industrial hygiene: real-time workplace air monitoring for VOCs using portable SPME-GC field kits
FAQ
What fiber lengths are available?
Standard configurations include 1 cm and 2 cm exposed fiber lengths; custom lengths may be accommodated upon technical review.
Are coating specifications provided with each batch?
Yes—each shipment includes a certificate of conformance listing coating type, thickness range, thermal stability limits, and batch-specific QC test results.
Can these fibers be used with thermal desorption GC inlets?
Yes, all standard INNOTEG SPME fibers are rated for direct thermal desorption at temperatures up to 250 °C (PDMS) or 300 °C (DVB/CAR/PDMS), consistent with common GC inlet operating ranges.
Is method validation support available?
INNOTEG provides application notes, spike-recovery data templates, and collaborative method transfer assistance for laboratories implementing SPME in regulated environments.
How should SPME fibers be stored and maintained?
Fibers must be stored in their original sealed vials at room temperature, protected from dust and UV light. Conditioning prior to first use (per coating-specific protocols) and routine blank checks are recommended to ensure longevity and reproducibility.
