INNOTEG EasySPE-Auto Fully Automated Solid Phase Extraction System

Overview

The INNOTEG EasySPE-Auto Fully Automated Solid Phase Extraction System is an engineered solution for high-throughput, reproducible sample preparation in analytical laboratories. Built upon classical solid-phase extraction (SPE) principles—comprising cartridge conditioning, sample loading, interferent washing, and analyte elution—the system replaces manual, labor-intensive workflows with a robust, programmable platform. It operates on positive-pressure liquid delivery via precision syringe pumps and multi-position solvent selection valves, ensuring consistent flow dynamics across all channels. Unlike vacuum manifold-based systems, the EasySPE-Auto maintains controlled, adjustable pressure during each step (conditioning, loading, washing, drying, elution), minimizing channel-to-channel variability and enhancing method transferability between labs. Its architecture supports large-volume water sample processing (up to 10 L per sample), making it suitable for trace organic analysis where pre-concentration and matrix removal are critical prior to GC, HPLC, or LC-MS/MS detection.

Key Features

• Multi-channel parallel processing: Simultaneous operation across 4–8 independent SPE channels, each with dedicated fluidic pathways, sealing caps, and pressure monitoring.
• Positive-pressure fluid control: High-accuracy syringe pumps deliver precise, pulse-free flow; integrated pressure sensors continuously monitor backpressure at each channel to detect blockages or cartridge clogging in real time.
• Low cross-contamination design: Individual needle-wash stations clean the dispensing needle after every aspiration and dispense step; each cartridge uses a dedicated, chemically inert sealing cap to prevent vapor-phase carryover and leakage.
• Optimized recovery performance: Programmable multi-step rinse cycles for sample tubes; extended solvent dwell time on cartridges during elution; optional post-elution nitrogen drying to remove residual solvents before collection.
• Minimal dead volume architecture: Direct-coupled syringe pump–valve manifold eliminates tubing dead volumes, reducing analyte loss and improving recovery for low-concentration targets.
• Intuitive touchscreen interface: Graphical workflow builder with drag-and-drop step sequencing; built-in method library with audit-trail logging compliant with GLP and 21 CFR Part 11 requirements (when paired with validated software configuration).

Sample Compatibility & Compliance

The EasySPE-Auto accommodates standard 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges—including silica-based, C18, HLB, SCX, SAX, and mixed-mode sorbents—from major vendors (e.g., Waters, Agilent, Thermo Fisher). It supports both aqueous and organic-rich matrices, including drinking water, wastewater, plasma, urine, milk, fruit juice, and soil extracts. The system complies with method requirements outlined in EPA Methods 508.1, 525.3, 549.2, and 8270; ISO 17993:2022 (water quality — determination of organic compounds); and USP chromatographic separations requiring rigorous sample cleanup. All hardware components contacting fluids are chemically resistant (PTFE, PEEK, stainless steel 316), supporting aggressive solvents such as dichloromethane, acetonitrile, and ethyl acetate without degradation.

Software & Data Management

The embedded control software provides full method development, execution, and review capabilities. Each run generates timestamped, user-attributed log files containing pump strokes, valve positions, pressure readings, and step durations. Audit trails record all parameter changes, user logins, and method modifications—enabling compliance with FDA 21 CFR Part 11 when deployed in regulated environments (e.g., clinical toxicology labs, environmental testing facilities accredited to ISO/IEC 17025). Raw data export is supported in CSV and XML formats for integration with LIMS platforms. Optional remote monitoring via Ethernet allows centralized instrument fleet management across multi-site laboratories.

Applications

• Residue analysis of pesticides, veterinary drugs, and mycotoxins in food commodities (e.g., fruits, cereals, meat, dairy)
• Trace-level quantification of pharmaceuticals, endocrine disruptors, and perfluoroalkyl substances (PFAS) in municipal and surface waters
• High-volume (1–10 L) enrichment of polar and semi-polar organics from drinking water per EPA Method 525.3
• Pre-concentration and cleanup of amphetamines, opioids, benzodiazepines, and synthetic cannabinoids in forensic toxicology specimens (urine, blood, oral fluid)
• Isolation of polyaromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and brominated flame retardants from sediment and sludge extracts

FAQ

What types of SPE cartridges are compatible with the EasySPE-Auto?
Standard commercially available 1 mL, 3 mL, 6 mL, and 12 mL cartridges from leading manufacturers—including reversed-phase (C18, C8), mixed-mode (HLB, MCX), ion-exchange (SCX, SAX), and specialized sorbents—are fully supported.
Can the system handle viscous or particulate-laden samples?
Yes—optional inline filtration modules and programmable flow-rate ramping allow gradual loading of turbid or high-viscosity matrices (e.g., plasma, homogenized tissue extracts) without column clogging.
Is method validation documentation available?
INNOTEG provides IQ/OQ protocols, performance qualification templates, and application notes aligned with ASTM D8255-20 and ISO/IEC 17025:2017 requirements for laboratory accreditation.
How is system maintenance tracked?
The software logs syringe actuation counts, valve cycle history, and seal wear indicators; automated alerts notify users when preventive maintenance intervals (e.g., syringe calibration, O-ring replacement) are due.
Does the system support unattended overnight operation?
Yes—fully autonomous batch runs with auto-shutdown, solvent level monitoring, and emergency pressure cutoff ensure safe extended operation without operator supervision.