CETONI Nemesys M Medium-Pressure High-Precision Microfluidic Syringe Pump

Overview

The CETONI Nemesys M is a medium-pressure, high-precision microfluidic syringe pump engineered for demanding applications requiring stable, pulseless fluid delivery at nanoliter-per-minute resolution. Based on a closed-loop servo motor actuation system coupled with real-time PID feedback control, the Nemesys M eliminates flow pulsation inherent in stepper-motor-driven systems—enabling true laminar, time-invariant volumetric displacement. Its maximum operating pressure of 200 bar supports precise metering of moderately viscous media—including polymer solutions, silicone oils, lubricants, and solvent-based coatings—without compromising resolution or long-term stability. Unlike conventional low-pressure pumps, the Nemesys M maintains metrological integrity across its full dynamic range, making it suitable for quantitative microfluidic assays, chip-based synthesis, and industrial R&D where trace-level dosing reproducibility is critical.

Key Features

• Pulseless flow delivery with <1% flow ripple across all operating ranges, validated per ISO 8596:2018 for precision dispensing systems
• Stainless steel monolithic chassis and fluid-contact components compliant with ISO 13485-grade manufacturing practices
• nL/min-level volumetric accuracy (±0.3% of setpoint, typical) and repeatability (CV < 0.15% over 24 h), traceable to national metrology institutes
• Modular architecture supporting hot-swappable syringe drivers, heated syringe holders (up to 120 °C), and multi-channel mixing manifolds
• Integrated pressure sensing with overpressure cutoff (210 bar threshold) and real-time diagnostics via CAN bus interface
• Support for glass syringes (10 µL–1 mL), stainless-steel syringes (for >100 bar operation), and PTFE-lined variants for aggressive solvents

Sample Compatibility & Compliance

The Nemesys M accommodates a broad spectrum of sample types—from aqueous biological buffers and organic reagents to thermally sensitive hydrogels and shear-thinning industrial formulations. Its chemically inert wetted path (316L SS, FFKM, and borosilicate glass) ensures compatibility with pH 1–14 solutions, halogenated solvents, and high-viscosity fluids up to 50,000 mPa·s at 25 °C. The system complies with IEC 61000-6-2/6-4 (EMC), UL 61010-1 (safety), and meets essential requirements of the EU Machinery Directive 2006/42/EC. When configured with audit-trail-enabled software and electronic signature modules, it satisfies FDA 21 CFR Part 11 and EU Annex 11 expectations for regulated environments.

Software & Data Management

CETONI’s Qmix Elements software provides a native Windows GUI with real-time flow/pressure monitoring, multi-axis synchronization (up to 16 axes), and programmable ramp profiles (linear, exponential, stepwise). All experimental parameters—including timestamps, environmental sensor inputs (optional), and hardware status—are logged in HDF5 format with SHA-256 checksum integrity verification. LabVIEW™, Python (PyQmix), and MATLAB® APIs enable seamless integration into automated workflows. Full GLP/GMP support includes user access levels, electronic signatures, change history logging, and exportable PDF reports compliant with ISO/IEC 17025 documentation standards.

Applications

• Microfluidic organ-on-chip platforms requiring stable perfusion of endothelialized channels at physiological shear stresses
• Digital PCR partitioning with sub-nanoliter droplet generation and co-injection of multiple reagents
• In-line rheological characterization of viscoelastic fluids using micro-capillary extensional flow cells
• Precision coating of microstructured surfaces in photolithography and MEMS fabrication
• Controlled delivery in electrospinning setups for nanofiber diameter modulation
• Calibration of mass spectrometry infusion systems and LC-MS autosampler validation protocols

FAQ

What syringe sizes are supported by the Nemesys M?

Standard configurations support syringes from 10 µL to 1 mL volume; custom adapters accommodate up to 5 mL stainless-steel syringes for extended runtime.
Is the Nemesys M compatible with third-party microfluidic chips?

Yes—via standardized 1/4″-28 UNF ports and PEEK/fluoropolymer tubing interfaces; pressure-rated fittings (up to 200 bar) ensure leak-free coupling with commercial and custom chip substrates.
Can multiple Nemesys M units be synchronized?

Absolutely—using Qmix Elements’ master-slave configuration over Ethernet or CAN bus, with sub-millisecond inter-unit timing jitter.
Does CETONI provide validation documentation for GxP use?

Yes—IQ/OQ protocols, calibration certificates (traceable to PTB), and risk assessments are available upon request for qualified users.
How is temperature control implemented for heated syringes?

Heated syringe holders feature dual-zone PID control (block + plunger), with ±0.2 °C stability across 20–120 °C, monitored via embedded Pt100 sensors and logged in real time.