SCIEX QTRAP 4000 Hybrid Triple Quadrupole/Linear Ion Trap Mass Spectrometer (Refurbished)

Overview

The SCIEX QTRAP 4000 is a hybrid mass spectrometer engineered for high-sensitivity quantitative and qualitative analysis in regulated and discovery-driven laboratories. It integrates a triple quadrupole mass filter with a linear ion trap (LIT) in a single collision cell architecture—enabling both high-duty-cycle MRM quantitation and information-dependent acquisition (IDA) for structural elucidation. Unlike conventional triple quadrupole instruments, the QTRAP 4000 leverages patented curved-linear collision cell design to eliminate low-mass cutoff, preserve ion transmission efficiency across the full m/z range (5–2000), and deliver high-fidelity MS/MS spectra suitable for spectral library matching (e.g., NIST, MassBank, METLIN). Its dual-mode operation supports simultaneous quantitative and qualitative workflows without hardware reconfiguration—making it especially valuable in ADME/Tox, clinical biomarker validation, forensic toxicology, environmental residue screening, and targeted metabolomics.

Key Features

• Hybrid scan functionality: seamless switching between MRM, enhanced product ion (EPI), precursor ion scan (PIS), neutral loss scan (NLS), and information-dependent acquisition (IDA)
• Linear ion trap with high ion storage capacity and rapid scan speed (≥10 Hz full-scan MS/MS)
• Triple quadrupole front-end optimized for ultra-low detection limits (sub-fg on-column sensitivity for peptides and small molecules under optimized LC conditions)
• Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources included; source geometry supports robust nanoflow to standard flow LC integration
• Collision-induced dissociation (CID) with adjustable collision energy for reproducible fragmentation patterns
• Integrated Turbo V™ ion source with heated nebulizer gas and curtain gas optimization for improved desolvation and signal stability

Sample Compatibility & Compliance

The QTRAP 4000 is routinely deployed in laboratories requiring compliance with international analytical standards including ASTM D7579 (petroleum biomarkers), ISO 17025 (testing laboratory competence), USP & EP monographs for impurity profiling, and FDA guidance for bioanalytical method validation (BMV). Its hardware architecture supports trace-level analysis of complex matrices—plasma, urine, tissue homogenates, soil extracts, food homogenates—without compromising specificity or dynamic range. All refurbished units undergo comprehensive functional verification per SCIEX-recommended protocols: mass accuracy calibration (≤0.2 Da RMS error), resolution verification (unit resolution at m/z 556), MRM precision testing (RSD ≤8% over 10 injections), and detector linearity assessment (≥4 orders of magnitude). Instruments are certified to meet original factory specifications for sensitivity, mass stability, and scan repeatability.

Software & Data Management

Controlled by Analyst® 1.7.1 (or later, upgradeable), the system provides validated tools for quantitative method development, peak integration, calibration curve fitting (linear/log-linear with weighting), and automated report generation. Analyst supports 21 CFR Part 11–compliant configurations when deployed with networked authentication, electronic signatures, and audit trail modules. Raw data files (.wiff) are natively compatible with third-party processing platforms including PeakView®, MasterView™, and open-source alternatives (XCMS, MZmine). Spectral libraries (e.g., NIST MS Search, METLIN) can be directly imported for automated compound identification during IDA acquisitions. All refurbished systems include licensed software media, activation keys, and documentation packages compliant with ISO/IEC 17025 record retention requirements.

Applications

• Pharmaceutical R&D: PK/PD studies, metabolite identification, impurity quantification per ICH Q2(R2)
• Clinical research: Biomarker verification, therapeutic drug monitoring (TDM), newborn screening assays
• Food safety: Pesticide multi-residue analysis, mycotoxin screening, veterinary drug residue confirmation
• Environmental monitoring: PFAS quantitation, pharmaceuticals in wastewater, dioxin congener profiling
• Forensic toxicology: Rapid screening and confirmation of novel psychoactive substances (NPS), opioids, and synthetic cannabinoids
• Academic proteomics: Targeted peptide quantitation (SRM/MRM), post-translational modification mapping

FAQ

Is this instrument fully refurbished and performance-verified?
Yes. Each QTRAP 4000 undergoes full electrical, vacuum, and ion-optical recalibration. Performance verification includes mass calibration, sensitivity validation using reserpine and angiotensin, and system suitability testing against SCIEX-defined acceptance criteria.
What level of technical support is provided post-purchase?
We provide remote troubleshooting, method transfer assistance, and on-site service coordination via authorized SCIEX field engineers. Extended service contracts (1–3 years) are available upon request.
Can the instrument interface with non-SCIEX liquid chromatography systems?
Yes. The QTRAP 4000 features standardized analog/digital I/O and API control interfaces compatible with Agilent 1290/1260, Shimadzu Nexera/XR, Waters Acquity/UPLC, and Thermo Vanquish systems via standard LC-MS trigger protocols.
Are consumables and spare parts available?
All major consumables—including ESI/APCI probes, turbo pump oil, collision cell components, and detector assemblies—are stocked and available with lead times under 5 business days.
Does the system include original documentation and certificates?
Yes. Each unit ships with original user manuals, maintenance logs, factory calibration reports, and a Certificate of Refurbishment signed by our ISO 9001-certified service team.