PerkinElmer Lambda 2S UV-Vis Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda 2S UV-Vis Spectrophotometer is a dual-beam, scanning spectrophotometer engineered for high-precision absorbance, transmittance, and reflectance measurements across the ultraviolet and visible spectral range (190–1100 nm). Based on double-monochromator optical architecture with holographic gratings and a low-stray-light design, the Lambda 2S delivers exceptional photometric accuracy (< ±0.002 A at 0.5 A), baseline stability (< ±0.0005 A/h), and wavelength repeatability (±0.1 nm). Its robust Czerny-Turner monochromator configuration, combined with a deuterium and tungsten-halogen lamp source, ensures long-term spectral fidelity and minimal drift—critical for quantitative analysis in regulated environments such as pharmaceutical QC labs, academic research, and environmental testing facilities.

Key Features

• Dual-beam optical design with real-time reference compensation for enhanced stability during kinetic or temperature-variable measurements
• Scanning speed up to 2400 nm/min with programmable scan modes (fixed-wavelength, wavelength-scan, time-drive, and quantitation)
• Integrated 8-position automated sample changer (standard configuration) supporting cuvettes from 0.1 mm to 100 mm pathlength
• High-sensitivity silicon photodiode detector with linear dynamic range of 0–3.5 A (absorbance)
• Front-panel keypad and LCD display for standalone operation; full remote control via USB 2.0 interface
• Pre-aligned optics and factory-calibrated wavelength/photometric scales—no user alignment required
• Refurbishment includes full optical inspection, lamp replacement (if aged >75% of rated life), slit-width verification, and system-level performance validation per PerkinElmer OEM specifications

Sample Compatibility & Compliance

The Lambda 2S accommodates standard 10 mm square quartz, glass, or plastic cuvettes, as well as microvolume adapters (e.g., 5 µL capillary cells) and solid-sample reflectance accessories (e.g., integrating sphere, specular reflectance stage). It supports compliance-critical workflows through built-in audit trail functionality (when used with optional WinUV software), enabling adherence to FDA 21 CFR Part 11 requirements for electronic records and signatures. Instrument calibration is traceable to NIST-standard reference materials, and routine verification follows ASTM E275 (Standard Practice for Description of Ultraviolet, Visible, and Near Infrared Spectrophotometers) and ISO/IEC 17025 guidelines for testing laboratories. All refurbished units undergo IQ/OQ documentation support upon request for GLP/GMP environments.

Software & Data Management

The Lambda 2S operates natively with PerkinElmer’s WinUV software suite (v7.x or later), providing comprehensive instrument control, spectral acquisition, multi-component quantitation (including Beer-Lambert regression, derivative spectroscopy, and peak integration), and report generation with customizable templates. Data files are saved in native .uv format and exportable to CSV, ASCII, or PDF for LIMS integration. The software supports method locking, user access levels (administrator/operator), and electronic signature capture—essential for regulated QA/QC applications. For labs requiring legacy compatibility, the instrument retains full RS-232 and analog output capability for integration into older SCADA or custom LabVIEW-based systems.

Applications

• Pharmaceutical assay development and dissolution testing per USP and
• Water quality analysis (nitrate, phosphate, COD, heavy metals via colorimetric methods)
• Enzyme kinetics and protein quantification (Bradford, Lowry, BCA assays)
• Polymer characterization (additive concentration, degradation monitoring)
• Nanoparticle size estimation via surface plasmon resonance (SPR) band analysis
• Educational laboratory instruction in analytical chemistry and physical biochemistry

FAQ

Is this Lambda 2S unit fully refurbished and performance-validated?

Yes. Each unit undergoes a standardized 12-point refurbishment protocol including optical alignment verification, lamp and detector diagnostics, wavelength accuracy testing using holmium oxide and didymium filters, and photometric linearity assessment with neutral density filters.
What documentation accompanies the instrument?

A Certificate of Inspection (COI) with pre- and post-refurbishment test data is provided. Optional IQ/OQ protocols and calibration certificates traceable to NIST standards are available upon request.
Can the Lambda 2S be integrated into an existing LIMS or ELN infrastructure?

Yes—via WinUV’s ODBC-compliant database export, direct CSV output, or third-party API connectors compatible with common laboratory informatics platforms.
Does the 6-month warranty cover parts, labor, and on-site service?

The warranty covers all parts and labor for defects in materials or workmanship. Remote technical support is included; on-site service is available under extended service agreements.
Are original accessories and manuals included?

All standard accessories—including cuvette holder, 10-mm quartz cuvettes (2 pcs), power cable, USB cable, and printed quick-start guide—are included. Full digital documentation (user manual, service manual, software installer) is delivered electronically.