Rainin UVIS 205 UV-Vis Absorbance Detector
| Origin | Canada |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | UVIS 205 |
| Price Range | USD $1,400–7,200 (FOB) |
| Product Category | HPLC UV-Vis Detector |
| Operational Age | 4–5 years |
| Warranty | 6 months limited |
Overview
The Rainin UVIS 205 is a high-stability, dual-beam ultraviolet-visible absorbance detector engineered for integration into legacy and modern high-performance liquid chromatography (HPLC) systems. Based on photodiode array (PDA)–compatible optical architecture with fixed-wavelength detection at 205 nm, the UVIS 205 delivers consistent baseline stability and low-noise signal output for routine quantitation of analytes exhibiting strong UV absorption—particularly peptides, carbonyl-containing compounds, and unsaturated organic molecules. Unlike variable-wavelength or full-spectrum PDA detectors, the UVIS 205 employs a precisely aligned mercury vapor lamp and interference filter assembly optimized for 205 nm operation, minimizing spectral drift and photometric error over extended run times. Its compact form factor, analog voltage output (0–1 V), and standard 1/4″–28 threaded flow cell interface ensure seamless compatibility with solvent delivery modules from Rainin, PerkinElmer, Waters, and Shimadzu HPLC platforms.
Key Features
- Fixed-wavelength detection at 205 nm—optimized for peptide bond and α,β-unsaturated carbonyl absorbance
- Dual-beam optical design with real-time reference compensation to suppress lamp intensity fluctuations and thermal drift
- High-sensitivity flow cell (10 µL pathlength, 1 mm internal diameter) constructed from fused silica and stainless steel for chemical inertness and pressure tolerance up to 4000 psi
- Analog output (0–1 V DC) compatible with legacy integrators, chart recorders, and modern DAQ systems via optional ADC interface
- Stabilized mercury arc lamp with >2,000-hour nominal lifetime; lamp status monitored via internal photodiode feedback
- Front-panel LED indicators for lamp warm-up, ready state, and flow cell blockage warning
- Reconditioned unit includes full optical alignment verification, electronic calibration against NIST-traceable standards, and functional validation using caffeine and uracil test solutions
Sample Compatibility & Compliance
The UVIS 205 supports aqueous and organic mobile phases commonly used in reversed-phase, ion-pair, and size-exclusion chromatography—including acetonitrile/water, methanol/water, TFA-containing buffers, and low-concentration ammonium acetate systems. Its flow cell design complies with ASTM D5292-17 (Standard Practice for Calibration of HPLC Detectors) and meets baseline noise and drift specifications outlined in USP Chromatography for system suitability assessment. All reconditioned units undergo GLP-aligned testing protocols: linearity verification across 0.01–2.0 AU range, limit of detection (LOD) determination per ICH Q2(R2), and retention time reproducibility assessment using certified reference standards. Documentation includes Certificate of Reconditioning, traceable calibration report, and compliance summary for internal audit readiness.
Software & Data Management
While the UVIS 205 operates as a standalone analog detector without native digital control, it integrates robustly into regulated environments through third-party data acquisition systems supporting FDA 21 CFR Part 11 compliance—including Empower 3 (Waters), Chromeleon 7 (Thermo Fisher), and OpenLab CDS (Agilent). SpectraLab Scientific provides configuration templates for analog input mapping, baseline correction algorithms, and peak area integration parameters validated against EP 10.0 and JP 18 methods. Audit trails, electronic signatures, and instrument qualification (IQ/OQ) documentation packages are available upon request. Firmware-level diagnostics—including lamp hours counter, detector temperature log, and voltage stability history—are accessible via RS-232 serial interface using proprietary terminal software (included).
Applications
- Quality control of synthetic peptides and oligonucleotides in pharmaceutical manufacturing (per ICH Q5C)
- Stability-indicating assays for β-lactam antibiotics under acidic hydrolysis conditions
- Quantitative analysis of aldehydes and ketones in food-grade solvents per AOAC 995.12
- Method transfer support for legacy HPLC methods originally developed on Rainin DYNAMAX or Perseptive Biosystems platforms
- Academic teaching labs requiring cost-effective, maintenance-light UV detection for introductory chromatography courses
- Environmental testing of nitroaromatic compounds (e.g., TNT, DNT) in soil extracts using EPA Method 8330B
FAQ
Is the UVIS 205 compatible with modern UHPLC systems?
Yes—when paired with a pressure-compatible flow cell upgrade kit (optional, part #UVIS-FC-UHPLC), it supports operating pressures up to 15,000 psi and dwell volumes <15 µL.
Does the 6-month warranty cover lamp replacement?
Yes—the warranty includes one complimentary mercury lamp replacement if failure occurs within the coverage period, subject to proof of proper warm-up/cool-down cycling.
Can I obtain IQ/OQ documentation for GMP use?
Yes—SpectraLab Scientific supplies vendor-qualified IQ/OQ protocols aligned with Annex 15 and ASTM E2500-13, including installation verification checklists and operational performance tests.
What calibration standards are used during reconditioning?
NIST-traceable caffeine (272 nm equivalent absorbance at 205 nm) and potassium dichromate solutions, with absorbance verified using a calibrated spectrophotometer per ISO/IEC 17025-accredited lab.
Is remote diagnostic support available post-purchase?
Yes—SpectraLab offers secure remote access (via TeamViewer Enterprise) for firmware diagnostics, analog output troubleshooting, and integration assistance with CDS platforms.
