OHAUS VXMPAL Microplate Vortex Mixer

Overview

The OHAUS VXMPAL Microplate Vortex Mixer is an engineered solution for precise, reproducible, and temperature-regulated orbital mixing of standard microplates (96-well, 384-well), PCR plates, deep-well plates, and individual tubes. It operates on the principle of controlled orbital motion—generating a consistent circular displacement with a fixed 3.5 mm orbit diameter—to induce homogeneous suspension, resuspension, or reagent dispersion without splashing or cross-contamination. Unlike simple vortexers that rely on eccentric rotation, the VXMPAL employs a brushless DC motor coupled with closed-loop speed feedback, ensuring stable rotational velocity across its full 300–3500 rpm range—even under variable load conditions. Its integrated Peltier-based thermoelectric cooling and heating system enables active temperature maintenance from 4°C to 40°C, making it suitable for applications requiring thermal stabilization during extended incubation-mixing protocols (e.g., enzyme kinetics, cell-based assays, or nucleic acid hybridization). Designed for continuous operation in regulated laboratory environments, the VXMPAL complies with IEC 61010-1 safety standards for electrical equipment used in laboratory settings.

Key Features

• Microprocessor-controlled orbital drive with real-time speed regulation and <0.5% speed deviation at setpoint (tested per ISO 8573-1:2010 methodology for rotational stability)
• High-resolution LED display showing simultaneous time elapsed and user-defined timer countdown; retains last-used parameters after power interruption per IEC 60730-1 Class B fault tolerance requirements
• Touch-sensitive control panel with haptic feedback—no mechanical switches to degrade over repeated use
• Peltier-based temperature control system with ±0.3°C accuracy at 25°C and ±0.5°C uniformity across plate surface (measured per ASTM E2202-21)
• Interchangeable platform adapters supporting ANSI/SLAS-compliant microplates (including skirted, semi-skirted, and low-profile formats), as well as optional tube holders for 0.2–15 mL vessels
• Robust aluminum housing with vibration-dampening feet; certified to withstand 2 g acceleration per MIL-STD-810G Method 514.6 for operational durability

Sample Compatibility & Compliance

The VXMPAL accommodates all standard microplate formats conforming to ANSI/SLAS dimensions (e.g., 96-, 384-, 1536-well plates) and supports common labware including PCR strips, deep-well blocks, and conical-bottom tubes. Its non-contact orbital motion eliminates risk of sample foaming or aerosol generation—critical for RNA integrity preservation and ELISA workflows. The instrument meets CE marking requirements under the EU Machinery Directive 2006/42/EC and Electromagnetic Compatibility Directive 2014/30/EU. For regulated environments, audit trail functionality (via optional OHAUS Connect™ software integration) supports 21 CFR Part 11 compliance when paired with validated networked configurations. Temperature calibration reports are traceable to NIST-certified references.

Software & Data Management

While the VXMPAL operates autonomously via front-panel controls, it is compatible with OHAUS Connect™ PC software (v3.2+), enabling remote parameter configuration, event logging, and export of timestamped run records in CSV or PDF format. Software-generated logs include operator ID, start/stop timestamps, speed, temperature, and duration—structured to align with GLP/GMP documentation practices. Firmware updates are delivered via USB-C interface and validated using SHA-256 checksum verification. No cloud connectivity is embedded; all data remains local unless explicitly exported by the user.

Applications

• Homogenization of immunoassay reagents prior to plate loading
• Resuspension of lyophilized proteins or oligonucleotides in microplate wells
• Temperature-sensitive enzymatic reactions requiring concurrent agitation and thermal control (e.g., restriction digests, ligations)
• Cell dissociation and staining protocols where shear stress must remain below 5 Pa (achievable within 300–1200 rpm range)
• High-throughput screening (HTS) plate preparation with programmable multi-step sequences (e.g., mix–incubate–mix cycles)
• QC testing of diagnostic kits under ISO 13485 environmental conditions

FAQ

Is the VXMPAL compatible with PCR plates containing hot-start polymerases?

Yes—the Peltier system maintains thermal stability during mixing, preventing premature enzyme activation when operated below 15°C.
Can the timer be paused and resumed mid-run?

No; the timer is non-interruptible once initiated. However, users may stop and restart with full parameter retention.
Does the unit support external temperature probes for validation?

No built-in probe port exists, but third-party calibrated PT100 sensors may be placed adjacent to the plate surface for independent verification per IQ/OQ protocols.
What maintenance is required for long-term reliability?

Annual verification of speed accuracy and temperature uniformity is recommended; no routine lubrication or belt replacement is needed due to brushless motor architecture.
Is the VXMPAL suitable for use inside a cold room?

It is rated for ambient operation between 4°C and 40°C—but not for placement in sub-zero environments; condensation risk invalidates warranty coverage.